大豆球蛋白对仔猪小肠上皮细胞Occludin mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2013.08.012

Abstract

Abstract:

This experiment was conducted to evaluate the effects of purified Glycinin on the permeability and the expression of tight junction protein Occludin in piglet intestinal epithelial cells (IPEC). IPEC were cultured with mediums added with Glycinin by different concentrations (0-5.0 mg·mL^-1) for 24 h. Measuring the mRNA expression of Occludin by real-time quantitative FQ-PCR and the transendothelial electrical resistant (TEER). The results showed that, compared with the control group, Glycinin could decrease the TEER and the relative expression of Occludin (P<0.001) mRNA in piglet, but it had no significant effect at the concentration of 0.5 mg·mL^-1. These results indicate that Glycinin can increase the permeability of the epithelial cells, and decrease the Occludin mRNA expression. Furthermore, this study clarify that the Glycinin directly induces intestinal damage by depressing intestinal epithelial cells growth and damaging the integrity of intestinal cells.

CLC Number:

S828
S811.2

HAN Rui, ZHAO Yuan, PAN Li, WANG Yu-qiong, QIN Gui-xin. Effects of Glycinin on Occludin mRNA Expression in Piglet Intestinal Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1258-1262.

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Effects of Glycinin on Occludin mRNA Expression in Piglet Intestinal Epithelial Cells

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