雄激素受体基因在绵羊公羔不同组织中的表达特性及性腺中的定位

doi:10.11843/j.issn.0366-6964.2013.08.011

Abstract

Abstract:

The objectives of this study were to evaluate the expression of Androgen receptors (AR) in various tissues and the cellular localization of gonad of the male sheep lambs. In this experiment, mRNA expression was detected by real-time fluorescent quantitative PCR. Cellular localization of AR in testis and epididymidis were examined by immunohistochemistry. Qualitative investigation of AR protein was expressed by Western blotting. The result showed that: (1) Sheep AR mRNA was expressed in all the tissues, and abundantly expressed in testis and heart. (2) Weak positive signals were detected in pseudo stratified ciliated columnar epithelium and interstitial tissue of EpH, EpB and EpT. Strong positive signals were detected in interstitial tissue, spermatogenous cells and myoid cells of sheep testis. Sheep AR was widely expressed in all the tissues. AR plays an important role in gonadal developing and sperm maturation processing and heart protecting, the mechanisms need further research.

CLC Number:

S826
Q786

LI Zhen, REN You-she, JIAO Guang-yue, BAI Yuan-sheng, QIN Xiao-wei, ZHANG Chun-xiang. The Expression of Androgen Receptor Gene in Different Tissues and Cellular Localization in Gonad of Male Sheep Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1251-1257.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.08.011

https://www.xmsyxb.com/EN/Y2013/V44/I8/1251

References

［1］ZHANG Q X, ZHANG X Y, ZHANG Z M, et al. Identification of testosterone-/androgen receptor-regulated genes in mouse Sertoli cells ［J］. Asian J Androl, 2012, 14: 294-300.

［2］MIYAMOTO J, MATSUMOTO T, SHIINA H, et al. The pituitary function of androgen receptor constitutes a glucocorticoid production circuit ［J］. Mol Cell Biol, 2007, 27: 4807-4814.

［3］KIMURA N, MIZOKAMI A, OONUMA T, et al. Immunocytochemical localization of androgen receptor with polyclonal antibody in paraffin-embedded human tissues ［J］. J Histochem Cytochem, 1993, 41: 671-678.

［4］SUAREZ-QUIAN C A, OKE B O, VORNBERGER W. Localisation du recepteur des andrognes dans le testicule ［J］. Andrology, 1997, 7: 293-304.

［5］BREMNER W J, MILLAR M R, SHARPE R M, et al. Immunohistochemical localization of androgen receptors in the rat testis: evidence for stage-dependent expression and regulation by androgens ［J］. Endocrinology, 1994, 135: 1227-1234.

［6］RAMESH K C, CRAIG M L, JOACHIM W, et al. Changes in endocrine profile and reproductive organs during puberty in the male marmoset monkey (Callithrix jacchus) ［J］. Reproduction, 2006, 132: 355-363.

［7］BERENSZTEIN E B, BAQUEDANO M S, GONZALEZ C R, et al. Expression of aromatase, estrogen receptor alpha and beta, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis ［J］. Pediatr Res, 2006, 60: 740-744.

［8］PEARL C A, MASON H, ROSER J F. Immunolocalization of estrogen receptor alpha, estrogen receptor beta and androgen receptor the pre-, peri- and post-pubertal stallion testis ［J］. Anim Reprod Sci, 2011, 125(1-4): 103-111.

［9］BOUKENAOUI N, MOUDILOU E, CHEVALIER C, et al. Postnatal changes in testicular development and androgen receptors immunolocalization in D’Man ram lambs ［J］. Folia Histochem Cytobiol, 2012, 50(1): 38-45.

［10］MATEESCU R G, THONNEY M L. Effect of testosterone on insulin-like growth factor-I, androgen receptor, and myostatin gene expression in splenius and semitendinosus muscles in sheep ［J］. J Anim Sci, 2005, 83(4): 803-812.

［11］S?OMCZY?SKA M, SZO?TYS M, DUDA M, et al. Androgen receptor in the reproductive systems of pregnant pigs and rats ［J］. Reprod Biol, 2006, 1: 113-121.

［12］TAKEDA H, CHODAK G, MUTCHNIK S, et al. Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor ［J］. J Endocrinol, 1990, 126: 17-25.

［13］ERIK H, ANDERS W, JOHNNY K, et al. Androgen receptor-beta mRNA levels in different tissues in breeding and post-breeding male and female sticklebacks, Gasterosteus aculeatus ［J］. Reprod Biol Endocrinol, 2012, 10: 23.

［14］LIU A Y, GAO L P, KANG S, et al. Testosterone enhances estradiols cardioprotection in ovariectomized rats ［J］. J Endocrinol, 2012, 212(1):61-69.

［15］MARSH J D, LEHMANN M H, RITCHIE R H, et al. Androgen receptors mediate hypertrophy in cardiac myocytes ［J］. Circulation, l998, 98: 256-261.

［16］KRIEG M, SMITH K, BARTSCH W. Demonstration of a specific androgen receptor in ratheart muscle: relationship between binding, metabolism, and tissue levels of androgens ［J］. Endocrinology, 1978, 103(5): 1686-1694.

［17］SHAN L X, BARDIN C W, HARDY M P. Immunohistochemical analy sis of androgen effects on androgen receptor expression in development Leydig and Sertoli cells ［J］. Endocrinology, 1997,138(3): 1259-1266.

［18］NAMIKI M, YOKOKAWA K, OKUYAMA A, et al. Evidence for the presence of androgen receptors in human Leydig cells ［J］. J Steroid Biochem Mol Biol, 1991, 8(1): 79-82.

［19］KIMURA N, MIZOKAMI A, OOUMA T, et al. Immunocytochemical localization of androgen receptor with polyclonal antibody in paraffin-embedded human tissues ［J］. J Histochem Cytochem, 1993, 41(5): 671-678.

［20］VORNBERGER W, PRINS G, MUSTO N A, et al. Androgen receptor distribution in rat testis: New implications for androgen regulation of spermatogenesis ［J］. Endocrinology, 1994, 134(5): 2307-2316.

［21］PELLETIER G, LABRIE C, LABRIE F. Localization of estrogen receptor alpha, estrogen receptor beta and androgen receptors in the rat reproductive organs ［J］. J Endocrinol, 2000, 165: 359-370.

［22］SAR M, LUBAN D B, FRENCH F S, et al. Immunohistochemical localization of the androgen receptor in rat and human tissues ［J］. Endocrinology, 1990, 127: 3180-3186.

［23］GOYAL H O, BARTOL F F, WILEY A A. Immunolocalization of androgen receptor and estrogen receptor in the developing testis and excurrent ducts of goats ［J］. Anat Rec, 1997, 249(1): 54-62.

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[3]	SONG Pengyan, WANG Siwei, YUE Qiaoxian, ZHANG Yinliang, CHEN Xiaoyong, ZHOU Rongyan. Identification of oar-miR-200b Promoter and Effects on Mitochondrial Function in Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5066-5076.
[4]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[5]	CHEN Conghui, DUAN Chunhui, YANG Xinyu, XIA Cui, GUO Yunxia, JI Shoukun, YAN Hui, LIU Yueqin, ZHANG Yingjie. Effects of Different Treatments of Transportation Stress on Energy Metabolism of Lambs Muscle after Slaughter [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5101-5111.
[6]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[7]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[8]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[9]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[10]	HE Mingyang, MA Yujing, WANG Yong, YANG Ruochen, LIU Yueqin, ZHANG Yingjie, DUAN Chunhui. Effects of Melatonin on Proliferation, Apoptosis of Ovarian Granulosa Cells, and Its Secretion of Steroid Hormones of Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3313-3324.
[11]	SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.
[12]	LI Yuexin, LIU Aiju, MA Xiaofei, ZHENG Zhong, HU Boxin, ZHI Yunxia, TIAN Shujun. Effect of TGFβR1 on the Function of Sheep Granulosa Cells Mediated by TGF-β/Smad Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3335-3347.
[13]	MA Youji, CHEN Pengfei, MA Qing, WU Yi. Effects of Different Exercise Amounts on Rumen Microflora Diversity of Tan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3393-3405.
[14]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[15]	XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.

The Expression of Androgen Receptor Gene in Different Tissues and Cellular Localization in Gonad of Male Sheep Lambs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments