Abstract:

To enhance immune response induced by DNA vaccines, porcine reproductive and respiratory syndrome (PRRS) DNA vaccines pCI-ORF5 was adsorbed on the surface of poly(lactide-co-glycolide) (PLGA) microparticles. The microparticles were prepared using a solvent evaporation method and the adsorption amount, in vitro release profiles and in vivo immunogenicity were evaluated. The results indicated that the DNA adsorbed on PLGA microparticles was about 0.9％ in 6 hours, and the release behaviors were influenced by some parameters such as CTAB content, PLGA molecular weight, PLGA concentration, internal aqueous phase volume. The PLGA microparticles with adsorbed DNA induced significantly enhanced humoral and cellular response in comparison to naked DNA in mice. It suggests that PLGA microparticles could be a promising vector for the delivery of DNA vaccines.