基于网络药理学分析饲粮添加蒲公英调节鹅免疫因子水平的作用机制

doi:10.11843/j.issn.0366-6964.2025.09.046

Abstract

Abstract:

The study aimed to investigate the mechanism of action by which the addition of dandelion to diets can enhance immune function in geese, using a network pharmacology approach. The Symmap database was used to collect the active ingredients and targets of dandelion. The Gene Cards database was used to search for immunosuppressive targets. The intersecting targets were subjected to protein-protein interaction (PPI) network analysis, Gene Ontology (GO) functional enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and molecular docking was performed between the core targets and the active components of dandelion. Subsequently, the outcomes of these analyses were used to conduct animal experiments to test the growth performance of the geese, immune-related indices, and relative expression of the core target mRNAs. The results showed that 190 key targets of dandelion to improve the immune function of geese were screened out through the database, and core targets, such as CASP3, IL-6, ACTB, and STAT3, were obtained by protein interaction analysis. GO and KEGG enrichment analyses showed that dandelion might be involved in the positive regulation of gene expression, response to hypoxia, negative regulation of apoptotic process, and carbohydrate metabolic process, and then exert immune regulatory effects through multiple signaling pathways such as FoxO signaling pathway, apoptosis, Toll-like receptor signaling pathway, MAPK signaling pathway, p53 signaling pathway, and NOD-like receptor signaling pathway. The results of molecular docking showed that the main active ingredients of dandelion had good docking activities with IL-6 and STAT3. The results of animal experiments showed that the addition of dandelion to the diet significantly increased growth performance and serum IgA and IgG levels, reduced IL-6 content, reduced the mRNA expression of IL-6 and STAT3, and increased the expression of FoxO1 and FoxO3 in the jejunal tissues of geese. This study demonstrated that adding dandelion to the diet could enhance the immune function of the organisms through the FoxO pathway, which provides a theoretical reference for the further development of dandelion as a feed supplement for geese.

Key words: dandelion, immunity, network pharmacology, molecular docking

CLC Number:

S853.74

ZHAO Yujie, WAN Baoxia, WANG Jiaqi, SUN Siyu, LENG Xinyang, CUI Yizhe. Mechanism of Action of Dandelion Addition to Ration to Enhance the Immune Performance of Geese Based on Network Pharmacological Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4698-4707.

Figures/Tables 13

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References 33

1	段惠春,蒲华云.蒲公英赛醇保护胃上皮细胞及抗炎作用的研究进展[J].中国当代医药,2022,29(20):30-34.
	DUANH C,PUH Y.Research progress of Taraxerol in protecting gastric epithelial cells and anti-inflammatory effect[J].China Modern Medicine,2022,29(20):30-34.
2	刘亦菲,刘兆薇,任一冉,等.蒲公英化学成分、药理作用研究进展及质量标志物预测分析[J].中华中医药学刊,2024,42(8):132-141, 291.
	LIUY F,LIUZ W,RENY R,et al.Research progress in chemical composition and pharmacological effects of Pugongying (Taraxacum officinale) and predictive analysis of quality markers[J].Chinese Archives of Traditional Chinese Medicine,2024,42(8):132-141, 291.
3	任汉书,朱文卿,郑媛媛,等.蒲公英的功能性成分及生物活性研究进展[J].食品与药品,2022,24(2):193-201.
	RENH S,ZHUW C,ZHENGY Y,et al.Research progress on functional components and biological activities of Taraxaci Herba[J].Food and Drug,2022,24(2):193-201.
4	付晨青,何立威,王秀萍,等.药食同源蒲公英的开发应用研究现状与展望[J].陕西农业科学,2021,67(5):86-88.
	FUC Q,HEL W,WANGX P,et al.Research status and prospect of development and application of medicinal and edible dandelion[J].Shaanxi Journal of Agricultural Sciences,2021,67(5):86-88.
5	程丽英,沈纪健,胡西阳,等.蒲公英的营养保健价值及其在食品中开发利用现状[J].食品工业,2022,43(8):331-334.
	CHENGL Y,SHENJ J,HUX Y,et al.Nutritional and health value of dandelion and its development and utilization in food[J].The Food Industry,2022,43(8):331-334.
6	郭梦宇. 丹参提取物、蒲公英提取物对肉鸡生产性能、肉品质、免疫和抗氧化机能的影响[D]. 保定: 河北农业大学, 2023.
	GUO M Y. Effects of Salvia miltiorrhiza extract and dandelion extract on production performance, meat quality, immunity and antioxidant function of broilers[D]. Baoding: Hebei Agricultural University, 2023. (in Chinese)
7	王留,刘秀玲.蒲公英水提物对肉仔鸡免疫功能和血清抗氧化功能的影响[J].饲料研究,2018(3):20-23.
	WANGL,LIUX L.Effects of dandelion water extract on immune function and serum antioxidant capacity of broiler chickens[J].Feed Research,2018(3):20-23.
8	邓海涛.日粮中添加蒲公英对肉鸡生长性能和抗病力的影响[J].国外畜牧学(猪与禽),2021,41(4):102-103.
	DENGH T.Effects of adding dandelion to feed on growth performance and disease resistance of broilers[J].Animal Science Abroad-Pigs and Poultry,2021,41(4):102-103.
9	唐诗博,高琳琳,吕树臣.蒲公英对贵妃鸡体重、免疫器官和经济效益的影响[J].黑龙江畜牧兽医,2018(22):65-67.
	TANGS B,GAOL L,LVS C.The efects of dandelion on the body weight, immune organs and economic benefits of the empress chicken[J].Heilongjiang Animal Science and Veterinary Medicine,2018(22):65-67.
10	世界中医药学会联合会.网络药理学评价方法指南[J].世界中医药,2021,16(4):527-532.
	SOCIETIES W F O C M.Network pharmacology evaluation methodology guidance[J].World Chinese Medicine,2021,16(4):527-532.
11	吕学泽,王梁,杨卫芳,等.当前我国畜牧业抗菌药应用与耐药性应对趋势[J].生物资源,2019,41(3):210-214.
	LVX Z,WANGL,YANGW F,et al.Current trend of antimicrobial drug application and drug resistance in animal husbandry in China[J].Biotic Resources,2019,41(3):210-214.
12	高中良,张丽英,李梦青,等.牵牛子中咖啡酸和咖啡酸乙酯的提取研究[J].安徽农业科学,2009,37(7):3049-3050.
	GAOZ L,ZHANGL Y,LIM Q,et al.Study on the extraction of caffeic acid and ethyl caffeate from semen pharbtidis[J].Journal of Anhui Agricultural Sciences,2009,37(7):3049-3050.
13	万春平, 祁燕, 郑喜, 等. 基于MAPK信号通路研究地胆草成分咖啡酸乙酯抑制T细胞活化的分子机制[C]//全国第十二届中西医结合风湿病学术会议论文汇编, F, 2014.
	WAN C P, QI Y, ZHENG X, et al. Research on the molecular mechanism of caffeic acid ethyl ester from Dachengcao suppressing T cell activation based on the MAPK signaling pathway[C]//Proceedings of the 12th National Conference on Integrated Traditional Chinese and Western Medicine for Rheumatism, F, 2014. (in Chinese)
14	KIMK B,NAMY A,KIMH S,et al.α-Linolenic acid: nutraceutical, pharmacological and toxicological evaluation[J].Food Chem Toxicol,2014,70,163-178. doi: 10.1016/j.fct.2014.05.009
15	杨家新. 槲皮素对AA肉鸡免疫功能的作用及机制[D]. 哈尔滨: 东北农业大学, 2018.
	YANG J X. The effects and mechanism of Quercetin on the immune function in AA broilers[D]. Harbin: Northeast Agricultural University, 2018. (in Chinese)
16	LUOC T,LIM O.Foxo transcription factors in T cell biology and tumor immunity[J].Semin Cancer Biol,2018,50,13-20. doi: 10.1016/j.semcancer.2018.04.006
17	QIW,QIW,XIONGD,et al.Quercetin: Its antioxidant mechanism, antibacterial properties and potential application in prevention and control of toxipathy[J].Molecules,2022,27(19):6545. doi: 10.3390/molecules27196545
18	董晓蒙. 槲皮素对脂多糖诱导肠炎抗炎效果研究[D]. 沈阳: 沈阳农业大学, 2022.
	DONG X M. Study on the Anti-inflammatory Effect of Quercetin on Lipopolysaccharide-Induced Enteritis[D]. Shenyang: Shenyang Agricultural University, 2022. (in Chinese)
19	KRAG,DADDAMJ R,MOALLEMU,et al.Alpha-linolenic acid modulates systemic and adipose tissue-specific insulin sensitivity, inflammation, and the endocannabinoid system in dairy cows[J].Sci Rep,2023,13(1):5280. doi: 10.1038/s41598-023-32433-7
20	KIMJ,AHNM,CHOIY,et al.Alpha-linolenic acid alleviates dextran sulfate sodium-induced ulcerative colitis in mice[J].Inflammation,2020,43(5):1876-1883. doi: 10.1007/s10753-020-01260-7
21	杨晰,张瑞.IL-6/STAT3通路与炎症相关结肠癌关系的研究进展[J].癌症进展,2018,16(9):1068-1070, 1139.
	YANGX,ZHANGR.Research progress on the relationship between IL-6/STAT3 pathway and inflammatory-related colorectal cancer[J].Oncology Progress,2018,16(9):1068-1070, 1139.
22	张锦鹏,王颢典,任华建,等.IL-6在相关疾病中作用机制的研究进展[J].医学研究与战创伤救治,2023,36(2):196-201.
	ZHANGJ P,WANGH D,RENH J,et al.Research progress on the mechanism of IL-6 in related diseases[J].Journal of Medical Research & Combat Trauma Care,2023,36(2):196-201.
23	毛凯荣,田岳凤,支博远,等.JAK2/STAT3信号通路调节机体免疫功能及针灸干预效应分析[J].中医药学报,2022,50(3):80-85.
	MAOK R,TIANY F,ZHIB Y,et al.Analysis of JAK2/STAT3 Signaling pathway regulating immune function and acupuncture intervention effect[J].Acta Chinese Medicine and Pharmacology,2022,50(3):80-85.
24	张天涵,沈洪.基于IL-6/JAK2/STAT3信号通路中药抗溃疡性结肠炎研究进展[J].环球中医药,2019,12(10):1600-1605.
	ZHANGT H,SHENH.Research progress of Chinese medicines for ulcerative colitis based on IL-6/JAK2/STAT3 signaling pathway[J].Global Traditional Chinese Medicine,2019,12(10):1600-1605.
25	LOH W,HSUS C,ALI-SEYEDM,et al.Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway[J].Cancer cell,2005,7(6):575-589. doi: 10.1016/j.ccr.2005.05.007
26	韩超,王丽娟,王鹏源,等.IL-6/JAK/STAT3信号通路在纤维化疾病中作用的研究进展[J].中国病理生理杂志,2022,38(12):2285-2290.
	HANC,WANGL J,WANGP Y,et al.Progress in role of IL-6/JAK/STAT3 signaling pathway in fibrotic diseases[J].Chinese Journal of Pathophysiology,2022,38(12):2285-2290.
27	OHH M,YUC R,DAMBUZAI,et al.STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4⁺ T cells[J].J Biol Chem,2012,287(36):30436-43. doi: 10.1074/jbc.M112.359661
28	LUNDELLL S,MASSARTJ,ALTINTAŞA,et al.Regulation of glucose uptake and inflammation markers by FOXO1 and FOXO3 in skeletal muscle[J].Mol Metab,2019,20,79-88. doi: 10.1016/j.molmet.2018.09.011
29	LINKW.Introduction to FOXO Biology[J].Methods Mol Biol,2019,1890,1-9.
30	CAOG,LINM,GUW,et al.The rules and regulatory mechanisms of FOXO3 on inflammation, metabolism, cell death and aging in hosts[J].Life Sci,2023,328,121877. doi: 10.1016/j.lfs.2023.121877
31	张娜,张紫燕,赵凌霞,等.FoxO1转录因子在巨噬细胞中的表达与炎症的关系[J].系统医学,2016,1(9):1-5, 46.
	ZHANGN,ZHANGZ Y,ZHAOL X,et al.The content change of FoxO1 transcription factor in macrophages and the relationship with inflammation[J].Systems Medicine,2016,1(9):1-5, 46.
32	KRAFCZYKN,KLOTZL O.FOXO transcription factors in antioxidant defense[J].IUBMB life,2022,74(1):53-61. doi: 10.1002/iub.2542
33	ZHANGB,HONGL,KEJ,et al.Polysaccharide of Atractylodes macrocephala Koidz alleviate lipopolysaccharide-induced liver injury in goslings via the p53 and FOXO pathways[J].Poult Sci,2023,102(3):102480. doi: 10.1016/j.psj.2023.102480

日粮组成 Ration composition	含量/% Content	营养成分 Nutrient composition	含量/% Content
玉米 Corn	61	代谢能/(MJ·kg^-1)^② Metabolic energy	11.04
豆粕 Soybean meal	21	粗蛋白质 Crude protein	16.14
稻壳 Rice husk	10	粗纤维 Crude fiber	3.04
植物油 Vegetable oil	0.5	中性洗涤纤维 Neutral detergent fiber	24.86
石粉 Stone powder	3	酸性洗涤纤维 Acid detergent fiber	14.35
预混料^① Premix	4	钙 Calcium	0.8
DL-蛋氨酸 DL-Methionine	0.27	磷 Phosphorus	0.5
磷酸氢钙 Calcium hydrogen phosphate	0.23	赖氨酸 Lysine	0.8
合计 Total	100	蛋氨酸 Methionine	0.45

名称 Name	序列 Sequence
IL-6	F: TGGCAACGACGATAAGGCAG
	R: TCGGAGGATGAGGTGTGTGG
STAT3	F: GGAGGAGGCGTTCGGGAAG
	R: GATGGTGTTGCTGAAGGAGGTG
FoxO1	F: TTCAATCCGTCACAACTTGTCTCTG
	R: GATGCCGCTCGCCTCCTC
FoxO3	F: GGACAACAGCAACAAGTACACC
	R: TGCTTGCCAAAATCGGTGAC

成分ID Ingredient ID	成分名称 Ingredient name	英文名称 English name	OB/%	编号 Number
SMIT05063	咖啡酸乙酯	Ethyl Caffeate	103.851	TM01
SMIT04878	毛茛黄素	Flavoxanthin	60.412 9	TM02
SMIT00013	槲皮素	Quercetin	46.433 3	TM03
SMIT00044	亚麻酸	Linolenic Acid	45.009 1	TM04
SMIT00042	甜菜碱	Betaine	40.922 3	TM05
SMIT01860	阿魏酸	Ferulic Acid	40.434 3	TM06
SMIT12514	果胶	Pectin	40.389 6	TM07
SMIT06410	菊黄质	Chrysanthemaxanthin	38.724 0	TM08
SMIT08158	蒲公英赛醇	Taraxerol	38.402 5	TM09
SMIT00034	β-谷甾醇	Beta-Sitosterol	36.913 9	TM10
SMIT00002	木犀草素	Luteolin	36.162 6	TM11
SMIT07872	咖啡酸甲酯	Methyl Caffeate	30.683 9	TM12

名称 Name	中介中心性 Betweenness	接近中心性 Closeness	度中心性 Degree
CASP3	2 016.759	0.003 135	55
IL6	1 775.099	0.003 058	52
ACTB	1 873.244	0.003 077	50
STAT3	1 154.264	0.003 067	50
CCND1	1 829.511	0.002 994	47
BCL2	905.7922	0.003 040	46
INS	3 610.713	0.002 985	44
GAPDH	1 694.433	0.003 003	43
CTNNB1	1 262.989	0.002 778	40
ESR1	1 830.851	0.002 933	39

项目 Item	蒲公英添加组 TM	对照组 CON	P值 P-value
初始体重/g Initial body weight	1 540.00±40.00	1 530.00±60.00	0.87
末体重/g Final body weight	3 571.00±67.00	2 925.00±30.00	＜0.01
平均日增重/g Average daily weight gain	72.62±3.31	50.00±2.50	＜0.01
平均日采食量/g Average daily feed intake	435.4±3.96	363.5±6.73	＜0.01
耗料增重比 Feed consumption to weight gain ratio	6.13±0.27	7.51±0.45	0.02

Mechanism of Action of Dandelion Addition to Ration to Enhance the Immune Performance of Geese Based on Network Pharmacological Analysis

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项目 Item	蒲公英添加组 TM	对照组 CON	P值 P-value
IgA	981.78±94.96	735.92±30.02	0.03
IgM	1 885.20±395.47	1 634.60±198.04	0.58
IgG	6 885.56±423.64	5 337.36±83.08	0.02
IL-6	35.53±2.16	43.04±1.17	0.02
C3	453.69±85.55	315.15±17.49	0.17
C4	653.18±51.89	523.70±34.81	0.07