狮头鹅群体遗传多样性和体重体尺全基因组关联分析

doi:10.11843/j.issn.0366-6964.2024.09.016

Abstract

Abstract:

The aim of this study was to investigate the genetic diversity of Lion-head geese and to perform the genome-wide association study of body weight and size traits. In this study, the 111 lion-head geese (20 males and 91 females) were randomly selected. They are two years old and off-season. The phenotypes detection of body weight and size traits and the second-generation genome sequencing (5×) for these geese were performed. SNPs from the sequencing data was called, the genetic diversity indexes was calculated and the association between SNPs and body weight and size traits was performed using the single SNP regression mixed model. The Bonferroni correction was used, i.e., the genome-wide threshold (0.05/a total of SNPs) and the chromosome-wide threshold (0.05/number of SNPs on each chromosome) to define the significant SNPs. The gene annotation was performed based on the 50 kb upstream and downstream of chromosome-wide significant SNPs. By the quality control, a total of 7 577 552 SNPs was obtained for the further analysis. The analysis of genetic diversity found that effective population size, minor allele frequency, polymorphic information content, observed heterozygosity, expected heterozygosity and inbreeding coefficient were 234, 0.25, 0.34, 0.26, 0.34 and 0.22, respectively. A total of 6 164 runs of homozygosity was detected, the high proportion (74.4%) of which ranged from 1.0 Mb to 2.0 Mb. We found 38 chromosome-wide significant SNPs by the association between SNPs and body weight and size traits, and these significant SNPs were involved in 90 genes. Among these genes, D2HDH, THAP4, ESPNL, EPT1, TNPO3, MCF2L and AIP1 were previously reported to affect the growth and development.In this study, by using the second generation genome sequencing data, we found a certain degree of inbreeding phenomenon for the Lion-head geese population and detected 7 candidate genes affecting the body weight and size traits, which can provide important references for the subsequent conservation of the Lion-head geese, the study of gene function and the application of molecular breeding.

Key words: Lion-head goose, population genetic diversity, body weight and size traits, genome-wide association study

CLC Number:

S835.2

Hongyan HUANG, Liyun ZHANG, Zhirong HUANG, Zhongping WU, Xumeng ZHANG, Hongjia OUYANG, Junpeng CHEN, Zhenping LIN, Yunbo TIAN, Xiujin LI, Yunmao HUANG. The Study on Population Genetic Diversity and Genome-wide Association Study of Body Weight and Size Traits for Lion-head Geese[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3914-3924.

Figures/Tables 8

Table 1

Fig. 1

Fig. 2

Table 2

Table 3

Fig. 3

Table 4

Significant SNPs loci"

性状 Trait	染色体 Chromosome	SNPs位置 Position	功能注释 Function annotation	候选基因 Gene
体重 Body weight	5	5:2300617	内含子	ACKA
	8	8:30133648	基因间	/
	9	9:11552267	基因上游	T4S1、NEUR4、ESPNL、G3ST2、THAP4、KTHY、ATG4B、D2HDH
	20	20:10823559	内含子	CABP8
胸宽 Chest width	8	8:6837235	内含子	TT39A、EPS15
胸宽 Chest width	14	14:17266712	基因间	/
胸深 Chest depth	1	1:147040959	内含子	PCID2、LAMP1、CUL4A、MCF2L、FA10
	2	2:47791095	基因间	/
	3	3:14051391	基因间	/
	4	4:59812177	基因间	/
	5	5:17426205	基因上游	RTJK、KS6A5、TTC7B
	20	20:8724007	基因间	RFC2、NTAL、CLIP2
骨盆宽 Pelvic width	14	14:1516505	基因间	RNF14、GNPI1
	18	18:3587225	内含子	CCG4、CCG1
	30	30:1992836	基因间	OPSB
体斜长Body-slant length	2	2:44820521	基因下游	EPT1、MARE2
龙骨长 Keel length	1	1:1229901	内含子	TNPO3、SND1
	1	1:48378687	内含子	RTJK、LKHA4、HUTI、HUTH
	18	18:7043981	基因下游	OTOP3、USH1G、OTOP2、HID1、FADS6、CDR2L
颈长 Neck length	10	10:15832904	内含子	MAGI1
	10	10:20923440	基因间	/
	14	14:10667386	基因间	DUS1
	14	14:10717636	内含子	RTJK
颈围 Neck circumference	2	2:8295313	基因间	SIA4A
	2	2:72691717	内含子	AIP1、RTJK
	21	21:7325312	内含子	TPRGL、WRP73、MEGF6
	22	22:424407	内含子	CLD22、ZBT16、5HT3A、UBP28
	29	29:3363012	基因上游	RPOB、POL、FV1
	30	30:1318061	基因下游	CP2G1、CP2C2、POL、ZC11A、O14J1
胫长 Tibia length	8	8:11781946	基因间	CA210、TIE1、ELOV1、CDC20、PIF1、EBP2、CFA57
	8	8:11929974	基因间	SIA7C
	8	8:14984264	内含子	DNS2B、VIT2、RPF1、URIC
	8	8:15008813	内含子	VIT2、RPF1
	10	10:8281363	内含子	ZMY10、TM115、C56D2、NPRL2、CA2D2
	16	16:1652251	内含子	ZBT46、TPD54、DNJC5
胫围 Tibia circumference	17	17:12971390	基因上游	VP37B、APC7、HIP1R、CCD62、AT2A2、GPN3
	18	18:11564724	基因间	FHAB、SHSA6
	30	30:1234187	内含子	CP2G1、CP2C2、DCAF8、CCD81

Table 4

Fig. 4

References 49

1	郭精奇. 饶平狮头鹅美誉传四方[J]. 源流, 2022, (8): 40- 41.
	GUO J Q . Raoping lion-head geese's good reputation spreads across the world[J]. Origins, 2022, (8): 40- 41.
2	刘思扬, 刘秋翔. 饶平狮头鹅[J]. 广东畜牧兽医科技, 2019, 44 (6): 10- 13. doi: 10.3969/j.issn.1005-8567.2019.06.004
	LIU S Y , LIU Q X . Lion-head goose from Raoping[J]. Guangdong Journal of Animal and Veterinary Science, 2019, 44 (6): 10- 13. doi: 10.3969/j.issn.1005-8567.2019.06.004
3	李国治, 邓卫东. 基因组测序技术及其应用研究进展[J]. 安徽农业科学, 2018, 46 (22): 20-22, 25. doi: 10.3969/j.issn.0517-6611.2018.22.006
	LI G Z , DENG W D . Research progress and application of genome sequencing technology[J]. Journal of Anhui Agricultural Sciences, 2018, 46 (22): 20-22, 25. doi: 10.3969/j.issn.0517-6611.2018.22.006
4	范广轩, 王洪亮, 邢秀梅. SNP标记的研究进展及其应用[J/OL]. 特产研究, 2023, doi: 10.16720/j.cnki.tcyj.2023.209.
	FAN G X, WANG H L, XING X M. Advances in SNP marker research and its applications[J/OL]. Special Wild Economic Animal and Plant Research, 2023, doi: 10.16720/j.cnki.tcyj.2023.209.(inChinese)
5	张顺进, 张花菊, 王红利, 等. 郏县红牛全基因组测序分析及关键基因SNP分子标记在育种的应用研究[J]. 中国牛业科学, 2021, 47 (5): 5- 8.
	ZHANG S J , ZHANG H J , WANG H L , et al. Whole genome sequencing analysis and application of key gene SNP molecular markers in Jiaxian red cattle breeding[J]. China Cattle Science, 2021, 47 (5): 5- 8.
6	齐丽娜, 陆雪林, 杨凯旋, 等. 基于SNP芯片分析新浦东鸡的遗传多样性和遗传结构[J]. 畜牧兽医学报, 2023, 54 (12): 4962- 4971. doi: 10.11843/j.issn.0366-6964.2023.12.008
	QI L N , LU X L , YANG K X , et al. Analysis of genetic diversity and genetic structure of New Pudong chicken based on SNP chips[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54 (12): 4962- 4971. doi: 10.11843/j.issn.0366-6964.2023.12.008
7	马钧, 樊安平, 王武生, 等. 全基因组重测序解析秦川牛保种群遗传多样性和遗传结构[J]. 遗传, 2023, 45 (7): 602- 616.
	MA J , FAN A P , WANG W S , et al. Analysis of genetic diversity and genetic structure of Qinchuan cattle conservation population using whole-genome resequencing[J]. Hereditas (Beijing), 2023, 45 (7): 602- 616.
8	曹宇浩. 湖羊体重性状GWAS分析与相关SNPs验证及快速检测方法的研究[D]. 南京: 南京农业大学, 2020.
	CAO Y H. Genome-wide association study of body weights in Hu sheep and related SNPs validation and rapid detection methods[D]. Nanjing: Nanjing Agricultural University, 2020. (in Chinese)
9	吕世杰, 陈付英, 张子敬, 等. 南阳牛生长性状相关基因组区域全基因组关联分析[J]. 中国畜牧兽医, 2020, 47 (1): 74- 82.
	LV S J , CHEN F Y , ZHANG Z J , et al. Genomic regions associated with growth traits in Nanyang cattle using genome-wide association analysis[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47 (1): 74- 82.
10	姜宏正, 荀文娟, 侯冠彧, 等. 家禽重要性状全基因组关联分析研究进展[J]. 黑龙江畜牧兽医, 2022, (11): 32- 38.
	JIANG H Z , XUN W J , HOU G Y , et al. Research progress of genome-wide association analysis of important traits in poultry[J]. Heilongjiang Animal Science and Veterinary Medicine, 2022, (11): 32- 38.
11	黎旺长, 刘玮玮, 龙佳佳, 等. 基于基因组重测序的广西地方猪种遗传多样性和选择信号分析[J]. 南方农业学报, 2023, 54 (8): 2415- 2422. doi: 10.3969/j.issn.2095-1191.2023.08.023
	LI W C , LIU W W , LONG J J , et al. Genetic diversity and selection signals analysis of Guangxi local pig breeds based on whole-genome resequencing[J]. Journal of Southern Agriculture, 2023, 54 (8): 2415- 2422. doi: 10.3969/j.issn.2095-1191.2023.08.023
12	田帅帅, 钟梓奇, 倪世恒, 等. 基于全基因组重测序数据对文昌鸡不同保种群保种现状的分析[J]. 黑龙江畜牧兽医, 2023, (13): 51-54, 62, 134.
	TIAN S S , ZHONG Z Q , NI S H , et al. Analysis of the conservation status of different conservancies of Wenchang chickens based on whole genome resequencing data[J]. Heilongjiang Animal Science and Veterinary Medicine, 2023, (13): 51-54, 62, 134.
13	蒋烈戈, 彭健, 代蓉, 等. 基于基因组SNP信息分析新疆夏洛莱牛群体的遗传结构和遗传背景初报[J]. 草食家畜, 2023, (1): 9- 15.
	JIANG L G , PENG J , DAI R , et al. Analysis of genetic structure and background of Xinjiang Charolais based on genomic SNP information[J]. Grass-Feeding Livestock, 2023, (1): 9- 15.
14	高超群, 曹然然, 杜文苹, 等. 基于全基因组SNP标记分析中国地方鸡品种的遗传多样性和种群结构[J]. 畜牧兽医学报, 2023, 54 (2): 554- 562. doi: 10.11843/j.issn.0366-6964.2023.02.013
	GAO C Q , CAO R R , DU W P , et al. Genetic diversity and population structure analysis of Chinese native chicken breeds using genome-wide SNPs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54 (2): 554- 562. doi: 10.11843/j.issn.0366-6964.2023.02.013
15	中华人民共和国农业农村部. NY/T 823—2020家禽生产性能名词术语和度量计算方法[S]. 北京: 中国农业出版社, 2020.
	Ministry of Agriculture and Rural Affairs of the People 's Republic of China. NY/T 823—2020 Performance terminology and measurements for poultry[S]. Beijing: China Agriculture Press, 2020. (in Chinese)
16	SONG H , CHU J Y , LI W J , et al. A novel approach utilizing domain adversarial neural networks for the detection and classification of selective sweeps[J]. Adv Sci (Weinh), 2024, 11 (14): 2304842. doi: 10.1002/advs.202304842
17	ZHANG K L , LIANG J T , FU Y H , et al. AGIDB: a versatile database for genotype imputation and variant decoding across species[J]. Nucleic Acids Res, 2024, 52 (D1): D835- D849. doi: 10.1093/nar/gkad913
18	DÍAZ-MATUS DE LA PARRA M , INOSTROZA K , ALCALDE J A , et al. Characterization of the genetic diversity, structure, and admixture of 7 Chilean chicken breeds[J]. Poult Sci, 2024, 103 (2): 103238. doi: 10.1016/j.psj.2023.103238
19	VISSCHER P M , WRAY N R , ZHANG Q , et al. 10 Years of GWAS discovery: biology, function, and translation[J]. Am J Hum Genet, 2017, 101 (1): 5- 22. doi: 10.1016/j.ajhg.2017.06.005
20	VANRADEN P M . Efficient methods to compute genomic predictions[J]. J Dairy Sci, 2008, 91 (11): 4414- 4423. doi: 10.3168/jds.2007-0980
21	ZHAO Q Q , LIN Z P , CHEN J P , et al. Chromosome-level genome assembly of goose provides insight into the adaptation and growth of local goose breeds[J]. GigaScience, 2022, 12, giad003. doi: 10.1093/gigascience/giad003
22	王海龙, 王巧, 邢思远, 等. 基于表型和基因组信息评价北京油鸡保种群保种情况[J]. 畜牧兽医学报, 2021, 52 (9): 2406- 2415. doi: 10.11843/j.issn.0366-6964.2021.09.004
	WANG H L , WANG Q , XING S Y , et al. Evaluating Beijing you chickens conservation status by phenotype and genome information[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52 (9): 2406- 2415. doi: 10.11843/j.issn.0366-6964.2021.09.004
23	季华员, 黄江南, 李海琴, 等. 兴国灰鹅微卫星标记的遗传多样性分析[J]. 畜牧与兽医, 2017, 49 (8): 6- 9.
	JI H Y , HUANG J N , LI H Q , et al. Genetic diversity analysis of Xingguo gray goose based on microsatellite markers[J]. Animal Husbandry & Veterinary Medicine, 2017, 49 (8): 6- 9.
24	郭徵力, 赵中龙, 杨红, 等. 织金白鹅微卫星标记遗传多样性及其与体尺指标的关联分析[J]. 农业生物技术学报, 2023, 31 (11): 2341- 2357. doi: 10.3969/j.issn.1674-7968.2023.11.012
	GUO Z L , ZHAO Z L , YANG H , et al. Genetic diversity of microsatellite markers in Zhijin white geese (Anser cygnoides orientalis) and its association analysis with body size indexes[J]. Journal of Agricultural Biotechnology, 2023, 31 (11): 2341- 2357. doi: 10.3969/j.issn.1674-7968.2023.11.012
25	刘继强, 郝晓东, 武丽娜, 等. 全基因组SNP分型技术在畜禽遗传育种研究中的应用[J]. 畜牧兽医学报, 2022, 53 (12): 4123- 4137. doi: 10.11843/j.issn.0366-6964.2022.12.001
	LIU J Q , HAO X D , WU L N , et al. Application of whole genome SNP genotyping technology in livestock and poultry genetics and breeding[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53 (12): 4123- 4137. doi: 10.11843/j.issn.0366-6964.2022.12.001
26	宋玉朴, 孙永峰, 冯自强, 等. SNP分型检测技术及其在畜禽遗传和育种中的应用研究进展[J]. 中国畜牧杂志, 2021, 57 (7): 37- 42.
	SONG Y P , SUN Y F , FENG Z Q , et al. Advances in SNP typing techniques and their application in genetic and breeding of livestock and poultry[J]. Chinese Journal of Animal Science, 2021, 57 (7): 37- 42.
27	胡紫平, 王立刚, 宗文成, 等. 基于基因组SNP和ROH的剑白香猪群体遗传结构解析[J]. 畜牧兽医学报, 2023, 54 (10): 4117- 4125. doi: 10.11843/j.issn.0366-6964.2023.10.011
	HU Z P , WANG L G , ZONG W C , et al. Genetic structure analysis of Jianbai Xiang pig population based on genomic SNP and ROH[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54 (10): 4117- 4125. doi: 10.11843/j.issn.0366-6964.2023.10.011
28	PHILLIPS E , SASARMAN F , SINASAC D S , et al. D-2-hydroxyglutaric aciduria in a patient with speech delay due to a novel homozygous deletion in the D2HGDH gene[J]. Mol Genet Metab Rep, 2019, 20, 100482. doi: 10.1016/j.ymgmr.2019.100482
29	YANG J , ZHU H W , ZHANG T L , et al. Structure, substrate specificity, and catalytic mechanism of human D-2-HGDH and insights into pathogenicity of disease-associated mutations[J]. Cell Discov, 2021, 7 (1): 3. doi: 10.1038/s41421-020-00227-0
30	DE GOEDE K E , HARBER K J , GORKI F S , et al. D-2-Hydroxyglutarate is an anti-inflammatory immunometabolite that accumulates in macrophages after TLR4 activation[J]. Biochim Biophys Acta Mol Basis Dis, 2022, 1868 (9): 166427. doi: 10.1016/j.bbadis.2022.166427
31	GERVAIS V , CAMPAGNE S , DURAND J , et al. NMR studies of a new family of DNA binding proteins: the THAP proteins[J]. J Biomol NMR, 2013, 56 (1): 3- 15. doi: 10.1007/s10858-012-9699-1
32	RICHTER A , HOLLSTEIN R , HEBERT E , et al. In-depth characterization of the homodimerization domain of the transcription factor THAP1 and dystonia-causing mutations therein[J]. J Mol Neurosci, 2017, 62 (1): 11- 16. doi: 10.1007/s12031-017-0904-2
33	SANGHAVI H M , MALLAJOSYULA S S , MAJUMDAR S . Classification of the human THAP protein family identifies an evolutionarily conserved coiled coil region[J]. BMC Struct Biol, 2019, 19 (1): 4. doi: 10.1186/s12900-019-0102-2
34	SALLES F T , MERRITT R C JR , MANOR U , et al. Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments[J]. Nat Cell Biol, 2009, 11 (4): 443- 450. doi: 10.1038/ncb1851
35	MERRITT R C , MANOR U , SALLES F T , et al. Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions[J]. Curr Biol, 2012, 22 (4): 320- 325. doi: 10.1016/j.cub.2011.12.053
36	EBRAHIM S , AVENARIUS M R , GRATI M , et al. Stereocilia-staircase spacing is influenced by myosin Ⅲ motors and their cargos espin-1 and espin-like[J]. Nat Commun, 2016, 7, 10833. doi: 10.1038/ncomms10833
37	KURZ S G , HANSEN K K , MCLAUGHLIN M T , et al. Tissue-specific actions of the Ept1, Ept2, Ept6, and Ept9 genetic determinants of responsiveness to estrogens in the female rat[J]. Endocrinology, 2008, 149 (8): 3850- 3859. doi: 10.1210/en.2008-0173
38	AHMED M Y , AL-KHAYAT A , AL-MURSHEDI F , et al. A mutation of EPT1 (SELENOI) underlies a new disorder of Kennedy pathway phospholipid biosynthesis[J]. Brain, 2017, 140 (3): 547- 554.
39	CHEN Y L , JIANG H G , ZHAN Z K , et al. Restoration of lipid homeostasis between TG and PE by the LXRα-ATGL/EPT1 axis ameliorates hepatosteatosis[J]. Cell Death Dis, 2023, 14 (2): 85. doi: 10.1038/s41419-023-05613-6
40	高广亮, 张克山, 赵献芝, 等. 全基因组关联分析筛选鹅蛋品质相关分子标记[J]. 中国农业科学, 2023, 56 (19): 3894- 3904. doi: 10.3864/j.issn.0578-1752.2023.19.015
	GAO G L , ZHANG K S , ZHAO X Z , et al. Identification of molecular markers associated with goose egg quality through genome-wide association analysis[J]. Scientia Agricultura Sinica, 2023, 56 (19): 3894- 3904. doi: 10.3864/j.issn.0578-1752.2023.19.015
41	BAYER M , BOLLER S , RAMAMOOTHY S , et al. Tnpo3 enables EBF1 function in conditions of antagonistic Notch signaling[J]. Genes Dev, 2022, 36 (15-16): 901- 915. doi: 10.1101/gad.349696.122
42	COSTA R , RODIA M T , PACILIO S , et al. LGMD D2 TNPO3-related: from clinical spectrum to pathogenetic mechanism[J]. Front Neurol, 2022, 13, 840683. doi: 10.3389/fneur.2022.840683
43	POYATOS-GARCÍA J , BLÁZQUEZ-BERNAL Á , SELVA-GIMÉNEZ M , et al. CRISPR-Cas9 editing of a TNPO3 mutation in a muscle cell model of limb-girdle muscular dystrophy type D2[J]. Mol Ther Nucleic Acids, 2023, 31, 324- 338. doi: 10.1016/j.omtn.2023.01.004
44	COSTA R , RODIA M T , ZINI N , et al. Morphological study of TNPO3 and SRSF1 interaction during myogenesis by combining confocal, structured illumination and electron microscopy analysis[J]. Mol Cell Biochem, 2021, 476 (4): 1797- 1811. doi: 10.1007/s11010-020-04023-y
45	DAY-WILLIAMS A G , SOUTHAM L , PANOUTSOPOULOU K , et al. A variant in MCF2L is associated with osteoarthritis[J]. Am J Hum Genet, 2011, 89 (3): 446- 450. doi: 10.1016/j.ajhg.2011.08.001
46	许永权, 张金山, 施纯南, 等. 骨性关节炎相关基因MCF2L可增加滑膜成纤维细胞凋亡及诱导炎症反应[J]. 基因组学与应用生物学, 2017, 36 (6): 2137- 2142.
	XU Y Q , ZHANG J S , SHI C N , et al. Osteoarthritis related genes MCF2L could increase apoptosis of synovial fibroblastsand induce inflammatory response[J]. Genomics and Applied Biology, 2017, 36 (6): 2137- 2142.
47	YANG L , LI Y Y , LING X X , et al. A common genetic variant (97906C>A) of DAB2IP/AIP1 is associated with an increased risk and early onset of lung cancer in Chinese males[J]. PLoS One, 2011, 6 (10): e26944. doi: 10.1371/journal.pone.0026944
48	LI Z , LI L , ZHANG H F , et al. Short AIP1 (ASK1-interacting protein-1) isoform localizes to the mitochondria and promotes vascular dysfunction[J]. Arterioscler Thromb Vasc Biol, 2020, 40 (1): 112- 127. doi: 10.1161/ATVBAHA.119.312976
49	LI Q Y , HUA X , LI L P , et al. AIP1 suppresses neovascularization by inhibiting the NOX4-induced NLRP3/NLRP6 imbalance in a murine corneal alkali burn model[J]. Cell Commun Signal, 2022, 20 (1): 59. doi: 10.1186/s12964-022-00877-5

测定指标 Trait	样本数量/只 Number	平均值 Mean	标准差 SD	最大值 Max	最小值 Min	变异系数/% C.V
体重/kg Body weight	111	6.11	0.94	8.63	3.98	15.45
胸宽/cm Chest width	111	116.99	5.35	138.61	104.03	4.58
胸深/cm Chest depth	111	117.39	5.44	134.60	106.36	4.63
胫长/cm Tibia length	111	102.69	4.10	113.94	92.10	3.99
骨盆宽/cm Pelvic width	111	101.36	4.07	107.65	69.93	4.02
颈围/cm Neck circumference	111	12.52	1.03	15.60	11.50	8.23
颈长/cm Neck length	111	35.30	2.35	42.30	32.50	6.66
体斜长/cm Body-slant length	111	36.95	1.74	41.80	33.80	4.72
龙骨长/cm Keel length	111	18.94	1.15	22.50	16.10	6.06
胫围/cm Tibia circumference	111	6.39	0.35	7.73	5.67	5.54

遗传多样性指标 The indexes of genetic diversity	平均值±标准差 Mean±SD
有效群体含量 Effective population size(Ne)	234(13世代以前)
最小等位基因频率 Minor allele frequency(MAF)	0.25±0.13
多态信息含量 Polymorphism information content(PIC)	0.34±0.13
观察杂合度 Observed heterozygosity(Ho)	0.26±0.11
期望杂合度 Expected heterozygosity(He)	0.34±0.13
近交系数 Inbreeding coefficient(F)	0.22±0.10

ROHs片段范围/Mb The length of ROHs	ROHs片段个数 Number of ROHs	所占比例% Proportion
1.0~2.0	4 583	74.4
2.0~3.0	1 016	16.5
3.0~6.0	477	7.7
>6.0	88	1.4

[1]	Ruiqi ZHANG, Yanqin PANG, Zaishan LI, Xiuguo SHANG, Ganqiu LAN, Jinbiao GUO, Yunxiang ZHAO. Research on Feeding Capacity Selection of Lactating Sows Based on Intelligent Precision Feeding [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 2890-2900.
[2]	CUI Shengdi, WANG Kai, ZHAO Zhenjian, CHEN Dong, SHEN Qi, YU Yang, WANG Junge, CHEN Ziyang, YU Shixin, CHEN Jiamiao, WANG Xiangfeng, TANG Guoqing. Identification of Candidate Genes for Pork Texture Traits Using GWAS Combined with Co-localisation of DNA Methylation [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1945-1957.
[3]	LI Keanning, DU Lili, AN Bingxing, DENG Tianyu, LIANG Mang, CAO Sheng, DU Yueying, XU Lingyang, GAO Xue, ZHANG Lupei, LI Junya, GAO Huijiang. Genetic Parameter Estimation and Genome-Wide Association Study for Carcass Traits and Primal Cuts Weight Traits in Huaxi Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3664-3676.
[4]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[5]	LI Hongwei, XU Lingyang, WANG Zezhao, CAI Wentao, ZHU Bo, CHEN Yan, GAO Xue, ZHANG Lupei, GAO Huijiang, LI Junya. Genome-wide Association Study of Slaughter Traits Based on Haplotype in Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4232-4243.
[6]	YANG Xinting, ZHENG Maiqing, TAN Xiaodong, ZHAO Guiping, HUANG Chao, LI Sen, LI Wei, WEN Jie, LIU Ranran. Genetic Parameters Estimation and Key Genes Identification for Meat Quality Traits of Fast-growing Yellow-feather Meat-type Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2416-2428.
[7]	OUYANG Fengzheng, WANG Ligang, YUE Jingwei, YAN Hua, ZHANG Longchao, HOU Xinhua, LIU Xin, WANG Lixian. Genome-wide Association Study of Copy Number Variations and Quantitative Trait Loci Mapping to Identify Candidate Genes for Body Height Trait in Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1515-1524.
[8]	LIU Xiaojing, LIU Lu, WANG Jie, CUI Huanxian, ZHAO Guiping, WEN Jie. Genome-wide Association Study of Chicken Blood Glucose Traits Using Whole Genome Resequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1187-1195.
[9]	WU Qun-qing, ZHANG Long-chao, HUANG Sheng-qiang, WANG Li-xian. A Genome-wide Association Study of Different Size Populations Based on Tail Analysis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1181-1190.
[10]	XU Pan，ZHANG Zhen，CUI Lei-lei，YANG Bin，DUAN Yan-yu. A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F₂ Resource Population [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 232-240.
[11]	HAO Xing-jie，HU Lin，ZHANG Shu-jun. Progresses in Research of Genome-wide Association Study Methods [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 213-217.
[12]	YANG Hui，FANG Shao-ming，HUANG Xiao-chang，CHEN Cong-ying，ZHANG Zhi-yan. Genome-Wide Association Study on Maternal Infanticide Behavior in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 241-248.
[13]	LAN Rong，ZHU Lan，YAO Xin-rong，WANG Peng，SHAO Qing-yong，HONG Qiong-hua. Genome-wide Association Study of Lambing Number in Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 549-554.
[14]	RUAN Guo-rong，LONG Yi，SU Ying，ZHANG Zhi-yan，XIAO Shi-jun，LIAO Xin-jun，AI Hua-shui，YANG Bin，DENG Zheng，XIN Wen-shui，TANG Jian-hong，REN Jun，DING Neng-shui，HUANG Lu-sheng. Genome-wide Association Study on Identifying Susceptibility Loci for Pig Inguinal/scrotal Hernia [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1775-1783.

The Study on Population Genetic Diversity and Genome-wide Association Study of Body Weight and Size Traits for Lion-head Geese

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 49

Related Articles 14

Recommended Articles

Metrics

Comments