HDLBP通过调控氧化应激水平和炎性因子表达参与鹅肥肝的形成

doi:10.11843/j.issn.0366-6964.2024.09.015

Abstract

Abstract:

The aim of this study was to investigate the subcellular localization and function of high density lipoprotein binding protein (HDLBP), and its relationship with the formation of goose fatty liver using in vivo and cellular models. Fourteen 70-day-old healthy Landes male geese were selected and raised in single cages. They were randomly divided into the control group (average weight 3.72 kg, ad libitum feeding) and the treating group (average weight 3.71 kg, overfed for 20 d) for in vivo experiment. Hepatocytes were isolated from 23-day-old Landes goose embryos and overexpressed with HDLBP gene for cellular experiment. Firstly, the subcellular localization of HDLBP protein in goose primary hepatocytes was determined by immunoblotting and immunofluorescence analyses. Secondly, the protein abundance of total HDLBP (wHDLBP) and mitochondrial HDLBP (mHDLBP) in the liver of the overfed geese and the control geese was determined by immunoblotting analysis. Then, HDLBP was overexpressed in goose primary hepatocytes, which was followed by determining the effects of HDLBP overexpression on the protein abundance of mHDLBP, the content of malondialdehyde (MDA), the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), the level of reactive oxygen species (ROS), and mitochondrial membrane potential. Finally, the differentially expressed genes (DEGs) and related signaling pathways affected by HDLBP overexpression were identified by transcriptome sequencing analysis, and some DEGs were verified in the in vivo model by quantitative polymerase chain reaction (PCR). The results showed that HDLBP could bind to mitochondria; the protein abundance of wHDLBP and mHDLBP in the overfeeding group was significantly lower than that in the control group (P < 0.01); overexpression of HDLBP in goose primary hepatocytes significantly increased the protein abundance of mHDLBP (P < 0.05), increased the levels of MDA (P < 0.01) and ROS (P < 0.05), and decreased the mitochondrial membrane potential (P < 0.05) and the activities of T-SOD (P < 0.05) and GSH-PX (P < 0.05). The up-regulated DEGs affected by HDLBP overexpression were mainly enriched in immune/inflammation-related pathways. In addition, compared with the control group, the expression of inflammation-related genes including IL1R1, TNFSF10, LTC4S, NCF1, SFTPA1, and KDR in the overfeeding group might be significantly reduced via HDLBP regulation (P < 0.05, 0.01 or 0.001). HDLBP can bind to mitochondria, and overfeeding significantly reduced the protein levels of wHDLBP and mHDLBP in goose liver. Overexpression of HDLBP leads to mitochondrial dysfunction, oxidative stress and increased expression of inflammatory factors. Therefore, HDLBP may provide protection for goose fatty liver by affecting mitochondrial function, regulating oxidative stress and inflammatory response.

Key words: goose, fatty liver, HDLBP, mitochondria, oxidative stress, inflammation

CLC Number:

S835.2

Zijin YUAN, Wanxin WANG, Ya XING, Jiahui LI, Ying XUE, Jing GE, Minmeng ZHAO, Long LIU, Daoqing GONG, Tuoyu GENG. HDLBP Is Involved in Goose Fatty Liver Formation by Regulating the Level of Oxidative Stress and the Expression of Inflammatory Factors[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3897-3913.

Figures/Tables 13

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Table 2

Fig. 7

Fig. 8

Fig. 9

Fig. 10

Fig. 11

References 38

1	刘同君, 赵盼, 赵敏孟, 等. 内质网应激标记基因Grp78参与鹅肥肝免疫/炎症状态的调控[J]. 畜牧兽医学报, 2019, 50 (4): 727- 737. doi: 10.11843/j.issn.0366-6964.2019.04.006
	LIU T Q , ZHANG P , ZHAO M M , et al. Endoplasmic reticulum stress marker gene Grp78 Is involved in regulation of immune/inflammatory state of goose fatty liver[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50 (4): 727- 737. doi: 10.11843/j.issn.0366-6964.2019.04.006
2	WEI R X , NING R , HAN C C , et al. Lipidomics analysis reveals new insights into the goose fatty liver formation[J]. Poult Sci, 2023, 102 (3): 102428. doi: 10.1016/j.psj.2022.102428
3	LIU L , WANG Q , WANG Q Q , et al. Role of miR29c in goose fatty liver is mediated by its target genes that are involved in energy homeostasis and cell growth[J]. BMC Vet Res, 2018, 14 (1): 325. doi: 10.1186/s12917-018-1653-3
4	柳序, 刘耀文, 匡佑华, 等. 鹅肥肝的形成及主要影响因素的研究进展[J]. 经济动物学报, 2019, 23 (4): 234- 239.
	LIU X , LIU Y W , KUANG Y H , et al. Advances on formation and main influencing factors of foie Gras[J]. Journal of Economic Animal, 2019, 23 (4): 234- 239.
5	GENG T Y , YANG B , LI F Y , et al. Identification of protective components that prevent the exacerbation of goose fatty liver: Characterization, expression and regulation of adiponectin receptors[J]. Comp Biochem Physiol B Biochem Mol Biol, 2016, 194-195, 32- 38. doi: 10.1016/j.cbpb.2016.01.006
6	XU A M , WANG Y , KESHAW H , et al. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice[J]. J Clin Invest, 2003, 112 (1): 91- 100. doi: 10.1172/JCI200317797
7	KASER S , MOSCHEN A , CAYON A , et al. Adiponectin and its receptors in non-alcoholic steatohepatitis[J]. Gut, 2005, 54 (1): 117- 121. doi: 10.1136/gut.2003.037010
8	STOWE D F , CAMARA A K S . Mitochondrial reactive oxygen species production in excitable cells: modulators of mitochondrial and cell function[J]. Antioxid Redox Signal, 2009, 11 (6): 1373- 1414. doi: 10.1089/ars.2008.2331
9	陈翠英, 邵明亮, 杨莉, 等. 乌丹降脂方对脂肪肝大鼠脂肪肝细胞氧化损伤调节作用研究[J]. 现代中西医结合杂志, 2016, 25 (20): 2180-2182, 2198. doi: 10.3969/j.issn.1008-8849.2016.20.005
	CHEN C Y , SHAO M L , YANG L , et al. Study on the regulation of Wudan Jiangzhi decoction on peroxidation damage in liver cells of the rats with liver fat[J]. Modern Journal of Integrated Traditional Chinese and Western Medicine, 2016, 25 (20): 2180-2182, 2198. doi: 10.3969/j.issn.1008-8849.2016.20.005
10	PENG K Y , WATT M J , RENSEN S , et al. Mitochondrial dysfunction-related lipid changes occur in nonalcoholic fatty liver disease progression[J]. J Lipid Res, 2018, 59 (10): 1977- 1986. doi: 10.1194/jlr.M085613
11	沈文婷, 陈明, 许诣, 等. PDTC调节线粒体功能障碍与Klotho蛋白表达对大鼠脓毒症合并急性肾损伤的影响[J]. 广西医科大学学报, 2021, 38 (11): 2097- 2103.
	SHEN W T , CHEN M , XU Y , et al. The effects of PDTC regulating mitochondrial dysfunction and Klotho protein expression on acute kidney injury in rat with sepsis[J]. Journal of Guangxi Medical University, 2021, 38 (11): 2097- 2103.
12	郭燕, 徐爽, 王亭. PKD1抑制剂CID755673通过诱导线粒体功能障碍加重急性肾损伤[J]. 临床与实验病理学杂志, 2023, 39 (2): 206-211, 215.
	GUO Y , XU S , WANG T . PKD1 inhibitor CID755673 aggravates acute kidney injury by inducing mitochondrial dysfunction[J]. Chinese Journal of Clinical and Experimental Pathology, 2023, 39 (2): 206-211, 215.
13	朱潇旭, 段小花, 李瑞霞, 等. 线粒体功能障碍与非酒精性脂肪肝发病关系的研究进展[J]. 山东医药, 2018, 58 (29): 108- 111.
	ZHU X X , DUAN X H , LI R X , et al. Research progress on the relationship between mitochondrial dysfunction and nonalcoholic fatty liver disease[J]. Shandong Medicine, 2018, 58 (29): 108- 111.
14	SUN Q Y , DAI E P , CHEN M , et al. Glucose-induced enhanced anti-oxidant activity inhibits apoptosis in goose fatty liver[J]. J Anim Sci, 2023, 101, skad059. doi: 10.1093/jas/skad059
15	FEICHT J , JANSEN R P . The high-density lipoprotein binding protein HDLBP is an unusual RNA-binding protein with multiple roles in cancer and disease[J]. RNA Biol, 2024, 21 (1): 1- 10.
16	CHENG M H K , JANSEN R P . A jack of all trades: the RNA-binding protein vigilin[J]. Wiley Interdiscip Rev RNA, 2017, 8 (6): e1448. doi: 10.1002/wrna.1448
17	DOMÍNGUEZ-PÉREZ M , SIMONI-NIEVES A , ROSALES P , et al. Cholesterol burden in the liver induces mitochondrial dynamic changes and resistance to apoptosis[J]. J Cell Physiol, 2019, 234 (5): 7213- 7223. doi: 10.1002/jcp.27474
18	ELUSTONDO P , MARTIN L A , KARTEN B . Mitochondrial cholesterol import[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 2017, 1862 (1): 90- 101.
19	DUAN Y J , GONG K , XU S W , et al. Regulation of cholesterol homeostasis in health and diseases: from mechanisms to targeted therapeutics[J]. Signal Transduct Target Ther, 2022, 7 (1): 265. doi: 10.1038/s41392-022-01125-5
20	洪胜辉, 张军, 张蕊, 等. 鹅原代肝细胞的简易、高纯分离及培养[J]. 江苏农业科学, 2012, 40 (4): 56- 58.
	HONG S H , ZHANG J , ZHANG R , et al. Simple, high-purity isolation and culture of goose primary hepatocytes[J]. Jiangsu Agricultural Sciences, 2012, 40 (4): 56- 58.
21	ZENG S , WU F , CHEN M Y , et al. Inhibition of fatty acid translocase (FAT/CD36) palmitoylation enhances hepatic fatty acid β-oxidation by increasing its localization to mitochondria and interaction with long-chain acyl-CoA synthetase 1[J]. Antioxid Redox Signal, 2022, 36 (16-18): 1081- 1100. doi: 10.1089/ars.2021.0157
22	MOBIN M B , GERSTBERGER S , TEUPSER D , et al. The RNA-binding protein vigilin regulates VLDL secretion through modulation of ApoB mRNA translation[J]. Nat Commun, 2016, 7 (1): 12848. doi: 10.1038/ncomms12848
23	GUO X Y , YIN X Z , LIU Z J , et al. Non-alcoholic fatty liver disease (NAFLD) pathogenesis and natural products for prevention and treatment[J]. Int J Mol Sci, 2022, 23 (24): 15489. doi: 10.3390/ijms232415489
24	周小艺, 邢娅, 龚道清, 等. 线粒体和鹅肥肝形成的关系[J]. 动物营养学报, 2023, 35 (2): 699- 707.
	ZHOU X Y , XING Y , GONG D Q , et al. Relationship between mitochondria and goose fatty liver formation[J]. Chinese Journal of Animal Nutrition, 2023, 35 (2): 699- 707.
25	DRAKE J C , WILSON R J , LAKER R C , et al. Mitochondria-localized AMPK responds to local energetics and contributes to exercise and energetic stress-induced mitophagy[J]. Proc Natl Acad Sci U S A, 2021, 118 (37): e2025932118.
26	ZHANG Q , DIDONATO J A , KARIN M , et al. BCL3 encodes a nuclear protein which can alter the subcellular location of NF-κB proteins[J]. Mol Cell Biol, 1994, 14 (6): 3915- 3926.
27	谢业成, 郭仪琳, 李雪露, 等. BCL3转录共激活因子的亚细胞定位研究[J]. 中国细胞生物学学报, 2021, 43 (8): 1574- 1580.
	XIE Y C , GUO Y L , LI X L , et al. Investigation on subcellular localization of BCL3 transcription coactivator[J]. Chinese Journal of Cell Biology, 2021, 43 (8): 1574- 1580.
28	VON ECKARDSTEIN A , NOFER J R , ASSMANN G . High density lipoproteins and arteriosclerosis.Role of cholesterol efflux and reverse cholesterol transport[J]. Arterioscler Thromb Vasc Biol, 2001, 21 (1): 13- 27.
29	郑吉春, 王韫芳, 裴雪涛. 胆汁酸代谢对肝细胞功能的影响[J]. 肝脏, 2007, 12 (4): 308- 310.
	ZHENG J C , WANG Y F , PEI X T . Effects of bile acids metabolism on hepatocytes function[J]. Chinese Hepatology, 2007, 12 (4): 308- 310.
30	MARTIN L A , KENNEDY B E , KARTEN B . Mitochondrial cholesterol: mechanisms of import and effects on mitochondrial function[J]. J Bioenerg Biomembr, 2016, 48 (2): 137- 151.
31	GOICOECHEA L , DE LA ROSA L C , TORRES S , et al. Mitochondrial cholesterol: Metabolism and impact on redox biology and disease[J]. Redox Biol, 2023, 61, 102643.
32	AHMAD S , YTTERBERG A J , THULASINGAM M , et al. Phosphorylation of leukotriene C₄ synthase at serine 36 impairs catalytic activity[J]. J Biol Chem, 2016, 291 (35): 18410- 18418.
33	WANG Y S , WANG J , ZHENG W J , et al. Identification of an IL-1 receptor mutation driving autoinflammation directs IL-1-targeted drug design[J]. Immunity, 2023, 56 (7): 1485- 1501.e7.
34	TANG W H , WANG W M , ZHANG Y X , et al. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced chemokine release in both TRAIL-resistant and TRAIL-sensitive cells via nuclear factor kappa B[J]. FEBS J, 2009, 276 (2): 581- 593.
35	HOLMDAHL R , SAREILA O , OLSSON L M , et al. Ncf1 polymorphism reveals oxidative regulation of autoimmune chronic inflammation[J]. Immunol Rev, 2016, 269 (1): 228- 247.
36	TOUAT-HAMICI Z , WEIDMANN H , BLUM Y , et al. Role of lipid phosphate phosphatase 3 in human aortic endothelial cell function[J]. Cardiovasc Res, 2016, 112 (3): 702- 713.
37	CUI Y N , ZHANG P P , LIANG X , et al. Association of KDR mutation with better clinical outcomes in pan-cancer for immune checkpoint inhibitors[J]. Am J Cancer Res, 2022, 12 (4): 1766- 1783.
38	THORENOOR N , ZHANG X S , UMSTEAD T M , et al. Differential effects of innate immune variants of surfactant protein-A1 (SFTPA1) and SP-A2 (SFTPA2) in airway function after Klebsiella pneumoniae infection and sex differences[J]. Respir Res, 2018, 19 (1): 23.

基因 Gene	引物序列(5′→3′) Primers sequence	产物大小/bp Product size
HDLBP	F: CCGTGGAGGTGAAGAAGTCC; R: CCTCGCAGTATCACCGTCTC	139
UBC	F: AGGGTGGATTCTTTCTGG; R: ACTGAGTTTGGAGGGAGC	243
GAPDH	F: CTGATGCTCCCATGTTCGT; R: CCACGATGCCAAAGTTGTCA	138
CHP1	F: TCCAGAAGGAGAGGACCAAGT; R: GGAGCTTGTTACTTCGGCTG	134
PCAN1	F: TTGCCTGTGTGGCAGATAGT; R: TGTGCAACTGGACTCTAGCAT	177
LOC106049357	F: TGGCAGATGCAGACACAACC; R: CACCCTGGCCTCTCGTAAAT	140
HRG	F: ACAAGGGATTCTGCAAGGCAC; R: TCTATGTCCATGGTGATGCTGCC	107
ELOVL6	F: GGTGGTCGGCACCTAATGAA； R: TCTGGTCACACACTGACTGC	169
GMPR	F: CTGCCAATCACCCAGAATGC; R: TCAGAGTAGCCGTTTGCCAC	142
ALDH1A1	F: GGCGACAAGGCAGATGTAGA; R: CAAGAGCCTTCCTCGCTCTG	105
MAT1A	F: TGCGCGTTCATACCATTGTG; R: GACCAGTAACACCAGCGTCA	198
PSAT1	F: AGAATGTTGGCTGTGCTGGA; R: AAAGAGCCATTCACCGCTTG	118
LOC106034448	F: ATCTGCTTGCTCCCTGGATT; R: TGTCCCTTTTCCATCTGCCA	187
SLC2A9	F: GCAGGGAAGGGCAATTGGT; R: GACCAGAGGAGAGTCAGGGT	194
IL1R1	F: GAACGCCCAGCTCAGAACAT; R: AAAGGATGGCACGAGTTCCG	140
LTC4S	F: TTCGAGCACAGGTGAACTCC; R: GAGGACGATGCCCTGTATCC	157
TNFSF10	F: TGTCCACAGGATAGCAGCAC; R: GGTATCACCAGCTCGCCATT	180
NCF1	F: GACACCTTCATCCGGCACA; R: TTTCGGTGAGGTCGTTCCAC	106
SFTPA1	F: GGCACACCTCTGAATTACACC; R: TGTGAGGCGATACAGGTTGC	126
KDR	F: ACCTGACGATGAACCCACAC; R: CCACATTCAGCTCCGTCCTT	90
PPAP2B	F: TGCTATCATCACGGGAGAGC; R: TGTGAAGGACTGGCTGATGG	142
SIGLEC1	F: CTTCAGCACCCAAAAGACCG; R: CAGGACATCCGAAACGCTCA	229
CCL20	F: TGAACAGCTCTCCAGTGAAGTC; R: TCCTTAGGGTTTACGCAGGC	87

	基因 Gene	变化倍数的对数 log₂(fold change)	P_adj
上调 Up-regulated	CP	2.97	2.38×10^-168
	TNIP3	4.39	1.00×10^-140
	LOC106046725	2.52	7.01×10^-134
	LOC106046905	2.30	2.85×10^-121
	NOXO1	5.09	3.23×10^-109
	SLCO4C1	4.76	8.57×10^-6
	STEAP4	5.20	3.21×10^-98
	CHN2	2.24	1.44×10^-96
	SLC13A5	9.04	3.98×10^-90
下调 Down-regulated	DPYS	-2.84	3.24×10^-153
	MAP3K7CL	-2.62	6.42×10^-132
	LOC106030908	-3.52	6.02×10^-116
	PCK1	-1.65	4.90×10^-69
	HRG	-1.51	1.52×10^-65
	TPH2	-2.96	2.26×10^-46
	LOC106034448	-1.52	6.91×10^-43
	GPR1	-1.39	1.94×10^-39
	LOC106048076	-2.67	4.63×10^-35
	CRACR2A	-1.43	4.83×10^-34

[1]	Shuo YANG, Min HUO, Zixuan SU, Yuxiang SHI. Research Progress on the Impact of Mitochondrial Quality Control on Oxidative Stress in Livestock and Poultry [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3769-3776.
[2]	HUANG Hongyan, ZHANG Liyun, HUANG Zhirong, WU Zhongping, ZHANG Xumeng, OUYANG Hongjia, CHEN Junpeng, LIN Zhenping, TIAN Yunbo, LI Xiujin, HUANG Yunmao. The Study on Population Genetic Diversity and Genome-wide Association Study of Body Weight and Size Traits for Lion-head Geese [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3914-3924.
[3]	WANG Yi, GONG Jianfei, HENG Nuo, HU Yingfan, WANG Rui, WANG Huan, ZHU Ni, HE Wei, HU Zhihui, HAO Haisheng, ZHU Huabin, ZHAO Shanjiang. Melatonin Alleviates Palmitic Acid-induced Damage in Bovine Endometrial Epithelial Cells by Improving Mitochondrial Dynamics [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3978-3987.
[4]	LIU Xinman, ZHOU Hongyuan, SANG Rui, GE Bingjie, YAN Kexin, WANG Wei, YU Minghong, LIU Xiaotong, QIU Qian, ZHANG Xuemei. Effect of Taraxasterol on Oxidative Stress in Liver Tissue of Broilers with AFB₁ Induced Liver Injury [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4141-4152.
[5]	Jingxuan WANG, Lizhi DAI, Zhenyu WANG, Ying LIU, Tong YU, Min YAN, Ruilong WANG, Jianhua XIAO. Study on the Characteristics of Liver Energy Metabolism during the Induction of Insulin Resistance by High Fat Diet [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4172-4185.
[6]	Yan WANG, Yadong GAO, Chenghui JIANG, Qiaoying ZENG. Isolation and Pathogenicity of a Goose Derived Fowl Adenovirus Type 4 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4232-4240.
[7]	Ya’nan LI, Tianwen MA, Yuhui MA, Chengwei WEI. Bilobalide Regulates Mitochondrial Biogenesis Mediated by AMPK-SIRT3 Positive Feedback Loop and Improves Inflammatory Damage of ATDC5 Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3714-3724.
[8]	Yaxuan MENG, Yan LIU, Jing WANG, Guoshun CHEN, Tao FENG. Research Progress in the Effect of Oxidative Stress on Ovarian Function in Female Livestock [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 2825-2835.
[9]	Yuanyuan LI, Tianyu WANG, Meng LI, Wenhui ZHANG, Yinghui WANG, Tianrui ZHAO, Haojie LI, Yangfei ZHAO, Jinming WANG. Selenomethionine, through PINK1/Parkin-mediated Mitochondrial Autophagy, Alleviates Fluoride-induced Depressive-like Behavior [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3213-3224.
[10]	Qianling CHEN, Yuzhu SHA, Xiu LIU, Pengyang SHAO, Fanxiong WANG, Xiaowei CHEN, Wenxin YANG, Zhuanhui XIE, Min GAO, Wei HUANG. Research Progress on the Interaction between Gut Microbiota and Mitochondria Regulating Animal Fat Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2293-2303.
[11]	CHEN Zhe, QU Xiaolu, GUO Binbin, SUN Xuefeng, YAN Leyan. Study on Candidate Genes for Green Light Affecting Early Development of Goose Embryo Heart Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1978-1988.
[12]	LI Feifei, ZHANG Chenmiao, TONG Jinjin, JIANG Linshu. Research Progress on the Mechanism of Mitochondrial Autophagy Regulating the Activity of NLRP3 Inflammatory Corpuscles to Improve Animal Health [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1446-1455.
[13]	ZHANG Xinting, QIU Wenyue, PANG Xiaoyue, SU Yiman, YE Jiali, HUANG Jianjia, ZHOU Shuilian, TANG Zhaoxin, WANG Rongmei, SU Rongsheng. Effect of Asiatic Acid Alleviating Myocardial Injury Caused by Lipopolysaccharide through Inhibiting Oxidative Stress and Ferroptosis in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1787-1799.
[14]	JIANG Lijun, ZONG Yunhe, LI Yunlei, CHEN Jilan, GENG Zhaoyu, SUN Yanyan, JIN Sihua. Research Progress of Antioxidant Application in Poultry Semen Storage [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 913-923.
[15]	SHEN Wenjuan, YANG Zhuo, ZHANG Xinrui, FU Yu, TAO Jinzhong. Research Progress of Microorganisms and Reproductive and Related Diseases in Dairy Cows Reproductive Tract [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 924-932.

HDLBP Is Involved in Goose Fatty Liver Formation by Regulating the Level of Oxidative Stress and the Expression of Inflammatory Factors

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 13

References 38

Related Articles 15

Recommended Articles

Metrics

Comments