Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (6): 2652-2661.doi: 10.11843/j.issn.0366-6964.2024.06.036
• Basic Veterinary Medicine • Previous Articles Next Articles
Dongliang LI1,2(), Guanmin ZHENG3, Shuai LI1, Hongsen ZHU3, Chao WU4,*(
)
Received:
2023-06-19
Online:
2024-06-23
Published:
2024-06-28
Contact:
Chao WU
E-mail:497222038@sian.com;wuchao_517@126.com
CLC Number:
Dongliang LI, Guanmin ZHENG, Shuai LI, Hongsen ZHU, Chao WU. Differential Expression of Transcriptome in Jejunal of Piglets Infected with Porcine Epidemic Diarrhea Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2652-2661.
Table 1
The primers used for RT-qPCR"
基因名称 Gene name | 上游引物(5′→3′) Forward primer | 下游引物(5′→3′) Reverse primer |
IL-8 | ACTGGCTGTTGCCTTCTTGG | CTGGCATCGAAGTTCTGCAC |
CIAP | GTTCAGTTAACATTTCCGGAGCC | GGTCAATGGCCACATCTGGAA |
NFKBIA | TCACCTACCAGGGCTACTCC | CCTCTGTGAACTCTGACTCCG |
SYK | GGACAGAAGCCATATCGGGG | AGCTCCACCGCTACAAATCC |
P13CABD | ACTGTGAACGAGCCTATGCC | CCGTTTATGGCTGTTGGGTTC |
IL-6 | GGTGATGGCTACTGCCTTCC | TGCAGAGATTTTGCCGAGGA |
RIGI | TTTCCACGAGGACGAAAGGG | GTGTCAGACGTTGCTCCAGA |
BAFF | AAAGCCCCTCTGTCCGCATC | CTGGTGGTGCAAAGATCCCTT |
IL-1β | AAGATAACACGCCCACCCTG | GAGTTTCCCAGGAAGACGGG |
CCL2 | GCGGCTGATGAGCTACAGAAG | CCGCGATGGTCTTGAAGATC |
CCL20 | GGTGCTGCTGCTCTACCTCT | GCTGTGTGAAGCCCATGATA |
CXCL2 | CCGTGCAAGGAATTCACCTC | TGCGGGGTTGAGACAAACTT |
CCL25 | GCCTACCACAGCCACATTAAG | GCTTCCCGCACACCATCTT |
CCL5 | ATCAGCCTCCCCATATGCCT | CCGCACCCATTTCTTCTCTG |
CCR7 | CAATTTCAACATCACCAGCGG | GAACTTGACGCCGATGAAGG |
CCL19 | GTTCGAGCCTTTCGCTACCT | GTGGTGCTTGCTCTTAGCAG |
CCL3L1 | CTTCCTCGCAAATTCGTAGC | GCATTCAGCTCCAGGTCAG |
CCL22 | TTACAGCTGCTCCCTGACAC | TGGATTGGAAGGGTAAGAGG |
GADPH | TCATCATCTCTGCCCCTTCT | GTCATGAGTCCCTCCACGAT |
Fig. 2
Identification of differentially expressed genes A. Volcano plots of differentially expressed genes between the mock-infected and PEDV-infected groups. The red, blue and gray dots represent up regulated, down regulated and nosing genes, respectively, in the PEDV-infected group compared with the mock infected group. B. Heatmap showing the expression levels of the differentially expressed genes. Columns represent individual samples (1, 2 and 3 were infected group; 4, 5 and 6 were control group) and rows indicate genes with significant expression differences between the two groups"
Table 2
Validation of jejunum differentially expressed genes by RT-qPCR"
基因 Gene | 基因ID GenBank ID | 转录基因log2FC RNA-seq log2FC | 荧光定量log2FC RT-qPCR log2FC |
IL-8 | NM_213867.1 | 2.08 | 1.34 |
CIAP | XM_021062707.1 | 2.38 | 0.90 |
NFKBIA | NM_001005150.1 | 1.50 | 0.94 |
SKY | NM_001104952.1 | 1.10 | 0.68 |
P13CABD | XM_021095292.1 | 1.79 | 2.54 |
IL-6 | NM_214399.1 | 4.76 | 4.23 |
RIGI | XM_021064058.1 | 1.47 | 1.57 |
BAFF | NM_001097498.1 | 1.61 | 1.46 |
IL-1β | XM_021085847.1 | 2.49 | 1.44 |
Table 3
Validation of chemotactic factor differentially expressed genes by RT-qPCR"
基因 Gene | 基因ID GenBank ID | 转录基因log2FC RNA-seq log2FC | 荧光定量log2FC RT-qPCR log2FC |
CCL2 | NM_214214.1 | 2.55 | 1.79 |
CCL20 | NM_001024589.1 | 2.21 | 1.17 |
CXCL2 | NM_001001861.2 | 2.56 | 2.50 |
CCL25 | XM_005661258.3 | -3.51 | -4.32 |
CCL5 | NM_001129946.1 | 0.38 | 0.63 |
CCR7 | NM_001001532.3 | 1.49 | 2.35 |
CCL19 | NM_001170516.1 | 2.39 | 1.81 |
CCL3L1 | NM_001009579.1 | 3.28 | 2.53 |
CCL22 | NM_001256776.2 | -1.02 | -0.45 |
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