柚皮苷可通过多种途径修复猪胸膜肺炎放线杆菌诱导的小鼠肺上皮屏障损伤

doi:10.11843/j.issn.0366-6964.2024.11.045

Abstract

Abstract:

The effect of naringin (NAR) on the prevention and treatment of lung epithelial barrier damage induced by Actinobacillus pleuropneumoniae (APP) infection in mice was investigated. Forty male Kunming mice between 6 and 8 weeks of age were randomly divided into five groups, namely, the control group, the model group, the NAR low-dose group, the NAR medium-dose group and the NAR high-dose group. The drug was administered by gavage continuously for 8 d. The blank and model groups were given saline. On d 7, mice were infected with APP (1×10⁸ CFU·mL^-1, 100 μL per mouse) by tracheal intubation, and the blank group was infused with an equal volume of sterile saline, and the mice in each group were executed at 48 h after modelling, sampled and evaluated for the preventive effect of NAR. X-ray images showed that NAR reduced the APP load in the alveolar lavage fluid of mice and alleviated the pathological lung injury; moreover, NAR intervention reversed the APP-induced decrease in the expression of ZO-1, Occludin-1, and Claudin-1 tight junction proteins in the lung tissues of mice, thus promoting the repair of the lung epithelial barrier. To further explore the mechanism of NAR promoting lung epithelial barrier repair, it was found that NAR not only reduced the secretion of pro-inflammatory factors IL-6 and TNF-α, but also increased the expression of anti-inflammatory factor IL-10, and increased the expression of antioxidant enzymes SOD2 and Hmox1 in mouse lung tissues, suggesting that NAR enhances the anti-inflammatory and antioxidant capacity of the organism. In addition, NAR can inhibit the TGF-β1/Smad2/3 pathway, reduce APP-induced proliferation of lung fibrotic tissue; analysis of key proteins related to apoptosis and Tunel fluorescent labelling revealed that NAR could reduce apoptosis in a dose-dependent manner. In conclusion, NAR can repair the lung epithelial barrier damage in mice through anti-inflammatory, antioxidant, anti-fibrotic and anti-apoptotic pathways, thus effectively reversing APP-induced inflammatory lung injury and exerting the efficacy of preventing and controlling APP.

Key words: natural medicine, naringin, Actinobacillus pleuropneumoniae, pig, lung epithelial barrier injury

CLC Number:

S853.74

Qilin HUANG, Chen LIU, Xiaohan JING, Lina HUANG, Xiangyi PAN, Xiongwei SHI, Zhengying QIU, Ruihua XIN. Naringin Repairs Actinobacillus pleuropneumoniae-induced Lung Epithelial Barrier Damage in Mice via Multiple Pathways[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5299-5309.

Figures/Tables 6

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引物名称 Primer name	上游引物(5′→3′) Forward sequence	下游引物(5′→3′) Reverse sequence	登录号 Accession No.
闭锁小带蛋白1 ZO-1	GCCGCTAAGAGCACAGCAA	TCCCCACTCTGAAAATGAGGA	NM_001163574
闭锁蛋白1 Occludin-1	TTGAAAGTCCACCTCCTTACAGA	CCGGATAAAAAGAGTACGCTGG	NM_008756
紧密连接蛋白1抗体(C端) Claudin-1	GGGGACAACATCGTGACCG	AGGAGTCGAAGACTTTGCACT	NM_01667
超氧化物歧化酶2 SOD2	TGCACTGAAATTCAATGGT	GTTTCTCCTTGAAGTTTGCG	NM_204211.1
血红素加氧酶1 Hmox1	GCTGAAGAAAATCGCCCAA	ATCTCAAGGGCATTCATTCGG	NM_205344.1
β-肌动蛋白 β-actin	GGTCACCAGGGCTGCTTT	ACTGTGCCGTTGACCTTGC	OY781925.1

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Naringin Repairs Actinobacillus pleuropneumoniae-induced Lung Epithelial Barrier Damage in Mice via Multiple Pathways

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