白屈菜红碱抗耐甲氧西林金黄色葡萄球菌作用机制研究

doi:10.11843/j.issn.0366-6964.2024.10.038

Abstract

Abstract:

The objective of this study was to investigate the antibacterial effect of chelerythrine (CHE) against methicillin-resistant Staphylococcus aureus (MRSA) and approach its mechanism. The antibacterial activity of CHE against MRSA was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). After treatment with CHE, the cell membrane and permeability of MRSA were assessed, and the morphology and ultrastructure of MRSA cells were observed by electron microscopy to preliminarily analyze the antibacterial mechanism of CHE. Additionally, the effect of CHE on reactive oxygen species (ROS) levels and ROS-related factors in MRSA strains was detected, combined with fluorescence quantification and transcriptomics to further explore the antibacterial mechanism of CHE. The results showed that the MIC of CHE against MRSA was 6.25 μg·mL^-1, MBC is 12.5 μg·mL^-1, and CHE can destroy cell membrane integrity and permeability to achieve antibacterial effect. CHE may block the electronic transmission of respiratory chain and promote ROS production by inhibiting the expression of gene SdhABC and other genes to exert activity against MRSA. In conclusion, this study explored the antibacterial mechanism of CHE against MRSA, which provided a new reference for clinical prevention of MRSA infection.

Key words: MRSA, chelerythrine, antibacterial effect, oxidative stress

CLC Number:

S853.74

Qingxin YUAN, Kuo LIU, Xuhua BAO, Dongyang GAO, He LI, Jun SONG, Zhixin ZHOU. The Mechanism of Chelerythrine against Methicillin-resistant Staphylococcus aureus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4670-4678.

Figures/Tables 8

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Table 2

Table 3

The top 20 annotated results of differentially expressed genes in KEGG database"

通路Pathway	一级分类Level 1	上调Up	下调Down
核糖体 Ribosome	遗传信息处理 Genetic information processing	0	49
光合作用Photosynthesis	新陈代谢Metabolism	0	8
精氨酸生物合成Arginine biosynthesis	新陈代谢Metabolism	6	8
半乳糖代谢Galactose metabolism	新陈代谢Metabolism	14	0
RNA聚合酶 RNA polymerase	遗传信息处理 Genetic information processing	0	5
细菌分泌系统 Bacterial secretion system	环境信息处理 Environmental information processing	4	6
双组分系统 Two-component system	环境信息处理 Environmental information processing	21	22
阳离子抗菌肽(CAMP)耐药性 Cationic antimicrobial peptide (CAMP) resistance	人类疾病 Human diseases	3	6
生物素代谢Biotin metabolism	新陈代谢Metabolism	5	3
O-抗原核苷酸糖生物合成 O-antigen nucleotide sugar biosynthesis	新陈代谢 Metabolism	7	2
阿特拉津降解Atrazine degradation	新陈代谢Metabolism	3	0
各种次生代谢产物的生物合成—第3部分 Biosynthesis of various secondary metabolites-part 3	新陈代谢 Metabolism	2	1
C5支链二元酸代谢 C5-branched dibasic acid metabolism	新陈代谢 Metabolism	0	7
ABC转运蛋白 ABC transporters	环境信息处理 Environmental information processing	28	16
蛋白质外排 Protein export	遗传信息处理 Genetic information processing	4	6
氧化磷酸化Oxidative phosphorylation	新陈代谢Metabolism	0	13
β内酰胺耐药性β-Lactam resistance	人类疾病Human diseases	0	6
幽门螺杆菌感染中的上皮细胞信号传导 Epithelial cell signaling in helicobacter pylori infection	人类疾病 Human diseases	1	1
植物-病原体相互作用Plant-pathogen interaction	生物系统Organismal systems	0	2
群体感应Quorum sensing	细胞过程Cellular processes	22	11

Table 3

Fig. 5

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基因Gene	序列(5'→3') Sequences	产物长度/bp Product
gene_01103	F: ACGGCAAAGAAGTGAATTACGA R: GCAAAGTGGAACACAACAGC	103
gene_01104	F: ACCGCTTAGGTGCCAATTCA R: GCATTTTCTGTACCGCGCAT	190

基因ID Gene ID	产物名称Production name	差异表达倍数Fold change
gene_01103	琥珀酸脱氢酶细胞色素b-558亚基(SdhC)	-1.32
	Succinate dehydrogenase cytochrome b-558 subunit
gene_01104	琥珀酸脱氢酶黄蛋白亚单位(SdhA)	-1.73
	Succinate dehydrogenase flavoprotein subunit

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The Mechanism of Chelerythrine against Methicillin-resistant Staphylococcus aureus

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