猪瘟病毒中和抗体与E2蛋白抗体相关性分析

doi:10.11843/j.issn.0366-6964.2024.10.030

Abstract

Abstract:

In order to explore the correlation between neutralizing antibody and E2 antibody against classical swine fever virus (CSFV), 289 swine sera were detected using both neutralization assay and ELISA among which neutralizing antibody titers of 15 sera were further tested by neutralization assay using different CSFV strains. The results showed that E2 antibody level against CSFV was positive correlated with neutralizing antibody titer. When the individual percentage inhibition of E2 antibody was over 60%, the neutralizing antibody titer of more than 70% vaccinated pigs in the herd meet the protection standard. These results could help the local veterinaries to evaluate the efficacy of CSFV vaccine better in the field and reduce the risk of CSF outbreak.

Key words: classical swine fever virus, neutralizing antibody, E2 antibody, correlation

CLC Number:

Yingju XIA, Yan LI, Jun LIU, Yuan XU, Fangtao LI, Xingqi ZOU, Qi LI, Jiaxin LI, Junjie ZHAO, Qianyi ZHANG, Yebing LIU, Lu XU. Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4590-4596.

Figures/Tables 6

Table 1

Table 2

Fig. 1

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Table 3

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References 24

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		E2抗体E2 antibody
		阳性 Positive	阴性 Negative	可疑 Suspicious	合计 Total	比率 Rate
中和抗体 Neutralizing antibody	阳性	201	12	10	223	0.772
	Positive	(a)	(b)	(e)	(a+b)	[r1=(a+b)/n]
	阴性	12	47	7	66	0.228
	Negative	(c)	(d)	(f)	(c+d)	[r2=(c+d)/n]
	合计	213	59	17	289
	total	(a+c)	(b+d)		(n=a+b+c+d+e+f)
	比率	0.737	0.204
	Rate	[r3=(a+c)/n]	[r4=(b+d)/n]

		E2抗体(阻断率) E2 antibody (PI)	中和效价对数 lgND₅₀
E2抗体(阻断率)	相关系数Pearson correlation	1	0.759^**
E2 antibody (PI)	显著性Sig. (2-tailed)		0.000
	N	289	289
中和效价对数	相关系数Pearson correlation	0.759^**	1
lgND₅₀	显著性Sig. (2-tailed)	0.000
	N	289	289

阻断率范围 Range of PI	中和效价对数lgND₅₀				免疫保护比例/% Percentage of protection
阻断率范围 Range of PI	0~1	1~1.5	≥1.5	合计Total	免疫保护比例/% Percentage of protection
0%~40%	54	7	15	76	19.7
40%~50%	4	11	11	26	42.3
50%~60%	5	6	24	35	68.6
60%~70%	2	5	18	25	72.0
70%~80%	1	5	35	41	85.4
80%~90%	0	1	55	56	98.2
90%~100%	0	0	30	30	100.0

血清编号 No. of serum	中和效价(ND₅₀)Neutralization titer			E2抗体E2 antibody
血清编号 No. of serum	SM (1.1亚型)	HuB (2.1亚型)	HeB (2.2亚型)	阻断率/% PI	判定 Results
1	1 280	240	640	91.00	阳性Positive
2	960	160	240	90.09	阳性Positive
3	1280	240	480	89.88	阳性Positive
4	60	60	40	69.45	阳性Positive
5	10	10	20	59.36	阳性Positive
6	20	40	60	56.97	阳性Positive
7	20	15	20	41.11	阳性Positive
8	N	40	20	39.82	可疑Doubtful
9	N	10	20	35.86	可疑Doubtful
10	10	N	N	35.32	可疑Doubtful
11	10	20	30	34.59	可疑Doubtful
12	10	10	N	27.87	阴性Negative
13	N	N	N	14.19	阴性Negative
14	N	15	N	10.62	阴性Negative
15	N	N	N	9.64	阴性Negative

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Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus

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