猪瘟病毒化学发光抗体检测方法的建立与应用

doi:10.11843/j.issn.0366-6964.2021.06.029

Abstract

Abstract: The aim of this study was to establish a chemiluminescence detection method for antibody against classical swine fever virus (CSFV), CSFV E2 protein was employed as the antigen, which was probed and bound by goat anti-pig IgG-HRP antibody. The signal was quantified by adding Luminol as the substrate solution. Compared to the commercial CSFV antibody detection kit, this method is characterized by fast detection (20 min at room temperature), equivalent sensitivity and great specificity, as evidenced by no cross-reaction with positive serum samples of A and O serotype of foot-and-mouth disease virus, porcine reproductive and respiratory syndrome virus, senecavirus A and African swine fever virus. Meanwhile, this method demonstrated high repeatability with low intraassay coefficient of variation (CV) (1.80%-6.88%) and interassay CV (1.11%-9.18%). The Kappa value of 152 pig serum samples collected from the field was 0.929, which was highly consistent with the results of the commercial CSFV antibody detection kit. In conclusion, the CSFV chemiluminescence antibody detection method established in this study displayed strong specificity, high sensitivity and repeatability, which can be applied to the clinical detection of CSFV serum antibody.

Key words: classical swine fever virus, chemiluminescence, antibody detection method

CLC Number:

S852.651

MA Yuan, SHI Zhengwang, LUO Juncong, YANG Bo, WANG Lijuan, WAN Ying, SONG Rui, CAO Liyan, ZHOU Gaijing, TIAN Hong, ZHENG Haixue, CHEN Yixia. Establishment and Application of Chemiluminescence Detection Method for Antibody against Classical Swine Fever Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1744-1752.

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Establishment and Application of Chemiluminescence Detection Method for Antibody against Classical Swine Fever Virus

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