靶向cofilin2基因RNAi对鸭病毒性肠炎病毒增殖的影响

doi:10.11843/j.issn.0366-6964.2022.03.032

Abstract

Abstract: This study aimed to investigate the effect of cofilin2 gene expression on the proliferation of duck enteritis virus (DEV). The complete gene sequence of cofilin2 from duck (GenBank:MF434775),four pairs of shRNA sequences were designed,inserted into vectors and transfected into duck embryo fibroblasts. shRNA sequences with high interference efficiency were screened by FQ-PCR and fluorescence microscope observation. The shRNA sequences with high interference efficiency were transfected into duck embryo fibroblasts to inhibit cofilin2 gene expression. Then check how cofilin2 is expressed during DEV replication. Result were as follows:Four pairs of shRNA interfering plasmids were successfully transfected into duck embryo fibroblasts and expressed by fluorescence microscopy. The silences were 4.96%±0.78%, 41.12%±2.94%, 17.55%±2.07%, 54.02%±1.73%, respectively, and cofilin2-86 was the most efficient. After cofilin2-86 transfection into duck embryo fibroblasts and DEV infection, the expression of DEV nucleic acid decreased significantly at different time. In conclusion, RNA interference targeting cofilin2 gene plays an important role in DEV replication and proliferation.

Key words: duck enteritis virus, DEV, RNA interference, cofilin2

CLC Number:

S852.659.1

WANG Wei, HUA Min, ZHANG Yun, LI Tao, ZHANG Qiandong, YUAN Yang, CHENG Zhentao, WEN Ming. Effect of RNAi Targeting cofilin2 Gene on Duck Enteritis Virus Proliferation[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 983-989.

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Effect of RNAi Targeting cofilin2 Gene on Duck Enteritis Virus Proliferation

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