非洲猪瘟病毒pS273R蛋白水解酶单克隆抗体制备

doi:10.11843/j.issn.0366-6964.2021.03.028

Abstract

Abstract: The objective of this study was to prepare monoclonal antibodies against the S273R protein of African swine fever virus (ASFV). BALB/c mice were immunized with purified recombinant S273R protein expressed by prokaryotic expression system and hybridoma cells were obtained by fusion of spleen cells and myeloma cells. An indirect ELISA based on the purified S273R protein was developed. Four hybridoma cell lines stably secreting MAbs against the S273R protein were harvested after screening and subcloning, and their sensitivity and specificity were detected by ELISA, Western blot, and immunofluorescence assay (IFA). Four MAbs heavy chains belong to IgG1, and light chains were κ. This research provided technical support for further study of biological functions of ASFV pS273R, as well as its gene-deletion based vaccine development.

Key words: African swine fever virus, S273R, prokaryotic expression, monoclonal antibody

CLC Number:

S852.659.1

ZHANG Meng, LIU Yingnan, XIE Zhenhua, AO Qingying, LÜ Lu, YU Wanqi, JIN Wenjie, QIN Aijian, HU Rongliang, CHEN Hongjun. Preparation of Monoclonal Antibodies against African Swine Fever Virus pS273R Protease[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 839-844.

References 14

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Preparation of Monoclonal Antibodies against African Swine Fever Virus pS273R Protease

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