大蒜素对小鼠黑色素瘤B16F10细胞体外增殖和迁移能力的影响

doi:10.11843/j.issn.0366-6964.2021.03.027

Abstract

Abstract: The study aimed to investigate the effects of different concentrations of allicin on the expression of microphthalmia-associated transcription factor (MITF) and its effects on the proliferation and migration of B16F10 cells, which provide a new idea for the treatment of animal melanoma in pet and animal husbandry. B16F10 cells were divided into control group (0 μg·mL^-1) and experimental group (5, 10, 15, 20 μg·mL^-1) supplemented with different concentrations of allicin. The expression of MITF mRNA in B16F10 cells was detected by qRT-PCR and the expression of MITF protein was detected by Western blot. The cell growth rate was calculated by using the absorbance of cells at 515 nm, and then the inhibition rate of different concentrations of allicin on cell proliferation was determined. Wound healing was used to evaluate the effects of different concentrations of allicin on the migration of B16F10 cells after 42 hours. The results showed that the expression of MITF mRNA decreased gradually with the increase of allicin concentration, while Western blot results showed that the expression of MITF protein decreased in a concentration-dependent manner. Allicin at the concentrations of 5, 10, 15, 20 μg·mL^-1 could significantly inhibit the proliferation of B16F10 cells (P<0.001), and the inhibition rate increased with the increase of allicin concentration. Wound healing results showed that allicin significantly inhibited the cell migration at the concentration of 10 μg·mL^-1, 15 μg·mL^-1 and 20 μg·mL^-1. This experiment showed that allicin could affect the expression of the melanoma-specific marker gene MITF and inhibit the proliferation and migration of B16F10 cells.

Key words: B16F10, MITF, allicin, proliferation, migration

CLC Number:

S857.4

ZHU Zhiwei, LAN Wutao, MA Yueyue, LI Pengfei, LI Hong, ZHANG Lei, SI Wenrui, FENG Jianghao. Effects of Allicin on Proliferation and Migration of Mouse Melanoma B16F10 Cells in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 831-838.

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Effects of Allicin on Proliferation and Migration of Mouse Melanoma B16F10 Cells in vitro

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