基于全基因组重测序技术的狮头鹅Indel标记分析

doi:10.11843/j.issn.0366-6964.2021.03.010

Abstract

Abstract: This study aimed to use resequencing technology to analyze the differences in Shitou goose gene Indel and to explore its egg production regulatory pathway. Five adult Shitou geese and five Jilin white geese with different egg production were selected, and the whole genome resequencing method was used to compare and analyze the indel mutations at the genome level of the two breeds. The results showed that 350.35 Gb data was obtained by sequencing, among which the average values of Q20 and Q30 reached 96.74% and 92.19%, respectively. Annotation analysis found 748 821.2 Indel mutation sites. The distribution trend and the number of Indel loci in the coding region (CDS) of 10 experimental geese were similar to those of the reference genome. Among them, 11 737 Indel loci resulted in insertion or deletion. On the basis, egg-laying related pathways were further analyzed and screened, and 15, 1, 1, 1, and 18 variant genes in the GnRH, estrogen, oxytocin, prolactin signaling pathways and progesterone-mediated oocyte maturation pathway, respectively involved in the regulation of goose egg-laying were found. The results suggest that the GnRH, estrogen, oxytocin, prolactin signaling pathways and progesterone-mediated oocyte maturation pathway are all related to Shitou goose egg-laying, and egg-laying related genes were further screened. These data will provide an effective basis for studying the reproductive performance of Shitou geese.

Key words: Shitou goose, resequencing, Indel, signaling pathway, egg-laying

CLC Number:

S835.2

WANG Huifang, ZHOU Guangxian, SUN Yongfeng, CHEN Xinqi, LEI Xubin, ZHANG Ruiyi, JIA Rumin, ZHAO Zhihui. Analysis of Indel Markers of Shitou Goose Based on Whole Genome Resequencing Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 662-675.

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Analysis of Indel Markers of Shitou Goose Based on Whole Genome Resequencing Technology

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