新城疫病毒复制对其溶瘤作用影响的初步分析

doi:10.11843/j.issn.0366-6964.2020.04.015

Abstract

Abstract: The aim of this study was to determine the effect of the replication level of Newcastle disease virus (NDV) on its oncolytic effect and to improve the research model for studying oncolytic mechanism of NDV. The human tumor cell line HCT116, A549 and the human non-tumor cell line HEK293T were infected with the NDV vaccine strain LaSota; the replication levels of viral genome in above mentioned cell lines were detected by NDV specific RT-qPCR; the expression levels of viral protein in above mentioned cell lines were assayed by Western blot; the effects of NDV infection on cell cycle and apoptosis of above mentioned cell lines were investigated using flow cytometry. The results revealed similar levels of viral replication but distinct oncolytic effects of NDV among these three cell lines; further flow cytometry showed that NDV infection induced G1 phase arrest in HEK293T and HCT116 but not in A549; in addition, NDV mainly induced early apoptosis in A549 but necrosis/late apoptosis in HCT116. The oncolytic effect of NDV does not depend on its replication levels, and NDV kills human tumor cells by cell type-specific mechanisms.

Key words: Newcastle disease virus, replication, oncolytic virus, cell cycle, apoptosis

CLC Number:

S852.659.5

WU Hanguang, SUN Junfeng, LIANG Yumeng, LIU Shengwang, LI Hai. Role of Viral Replication in the Oncolysis of Newcastle Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 794-800.

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Role of Viral Replication in the Oncolysis of Newcastle Disease Virus

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