麦冬多糖脂质体增强雏鸡免疫功能的分析

doi:10.11843/j.issn.0366-6964.2015.07.023

Abstract

Abstract:

In order to investigate the immune-enhancing activity of ophiopogon polysaccharide liposome (OPL)，two experiments were carried out.In vitro，the effects of OPL on splenocyte proliferation of chicken were determined by MTT method，and ophiopogon polysaccharide (OP) was used as control.In vivo，four hundred 11-day-old chickens were randomly assigned into 8 groups，and the chickens were injected with cyclophosphamide to build the immunosuppression model.At 14-day-old，the chickens were vaccinated with Newcastle disease vaccine.At the same time of the first vaccination，the chickens were injected respectively with OPL at different dosages.At various time points after the first vaccination，the peripheral lymphocyte proliferation，serum antibody titer and the contents of cytokines were measured.The results showed that OPL could significantly promote splenocyte proliferation singly or synergistically with PHA and LPS at 7.813-125 μg•mL^-1 in vitro，and the efficacy was superior to OP (P<0.05)；In vivo，OPL at high and medium doses could significantly promote lymphocyte proliferation，enhance antibody titer and improve the contents of IL-2 and IFN-γ compared with OP at most time points (P<0.05).These results indicated that OPL could significantly improve the immune-enhancing activity of OP，OPL would be expected to exploit into a new-type preparation of OP.

CLC Number:

S853.74

FAN Yun-peng，MA Lin，ZHANG Wei-min，SONG Xiao-ping，GUO Chao，HOU Wei-feng，TONG De-wen. Analysis of Ophiopogon Polysaccharide Liposome on Enhancing the Immune Function in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1246-1252.

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Analysis of Ophiopogon Polysaccharide Liposome on Enhancing the Immune Function in Chickens

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