FSH和胰岛素对绵羊卵巢卵泡颗粒细胞体外培养的影响

doi:10.11843/j.issn.0366-6964.2013.09.008

Abstract

Abstract:

The study focused on the effect of FSH and insulin on the proliferation of granulosa cells and the production of estradiol when the sheep follicle granulosa cells were cultured in serum-free medium. Granulosa cells were collected and treated with a range of concentration of FSH and insulin, and cultured for 168 h using long term method. Observed and counted under the microscope. Estradiol concentration was measured by double antibody ABC-ELISA. Results indicated that granulosa cell proliferation and estradiol production in the groups treated with FSH and insulin were superior to that in other groups treated alone. At 10 ng·mL^-1 concentration of insulin and 5.0 or 25.0 ng·mL^-1 concentration of FSH in medium, the number of granulosa cells were most and there is no significant difference between these two treated groups; at 10 ng·mL^-1 concentration of insulin and 5.0 ng·mL^-1 concentration of FSH in medium, Estradiol concentration was the highest (P<0.05) and the culture system was optimal. The multiplication and secretion of estradiol in sheep ovarian granulosa cells were induced by FSH. Insulin promotes the multiplication and estradiol secretion of FSH-induced sheep follicular granulosa cells in vitro. FSH and insulin stimulate the multiplication and estradiol secretion of sheep follicular granulose cells in a concentration-dependent manner.

CLC Number:

S826
S814.1

LI Peng-fei, YUE Wen-bin, PANG Yu-ying, YU Xue-jing, HUANG Yang, REN You-she, LYU Li-hua. Effects of FSH and Insulin on Sheep Ovarian Follicular Granulosa Cells in vitro Culture[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1386-1391.

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Effects of FSH and Insulin on Sheep Ovarian Follicular Granulosa Cells in vitro Culture

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