山羊UBA52基因组织表达规律及其与性早熟的关联分析

doi:10.11843/j.issn.0366-6964.2013.09.007

Abstract

Abstract:

In order to detect the association of UBA52 gene and sexual precocity in goats, female sexual precocious goat breed Jining Grey goat and female sexual late-maturing goat breed Liaoning Cashmere goat were selected. RT-PCR method was used to detect the expression profile of UBA52 gene in tissues at different developmental stages of the two breeds，and then real-time PCR technology was used to exam the mRNA expression level of UBA52 genes in hypothalamus, pituitary gland, ovary, uterus and adrenal gland. The result showed that UBA52 gene was widely expressed in the organizations at different developmental stages of the two breeds, and its expression level in the uterus was the highest.The results also showed that the expression level of UBA52 in pituitary gland in puberty was significantly lower than that in juvenile period of Jining Grey goat（P<0.01）, while the expression level in juvenile period Ⅱwas significantly higher than that in juvenile period Ⅰof Liaoning Cashmere goat, and its expression level in puberty was significantly lower than that in juvenile period Ⅱ（P<0.01）. The results indicated that the up-regulated expression of UBA52 gene was negatively correlated to goats estrus. So it was concluded that the up-regulation of UBA52 may reduce the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) to suppress the occurrence of goat puberty.

CLC Number:

S827
S813.3

WANG Yao-wu, WANG Yong-juan, CHU Ming-xing, CAO Gui-ling, WANG Jin-yu, DI Ran, LIU Qiu-yue. Tissue Expression of UBA52 Gene and Its Association with Sexual Precocity in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1380-1385.

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Tissue Expression of UBA52 Gene and Its Association with Sexual Precocity in Goats

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