精子蛋白32的表达及酪氨酸磷酸化调控猪精子顶体蛋白的活化

doi:10.11843/j.issn.0366-6964.2013.08.008

Abstract

Abstract:

The aim of this study was to investigate the relationship between the expression level,tyrosine phosphorylation of sp32 and the activation of the boar proacrosin/acrosin system, the acrosomal membrane proteins of boar sperm for different treatments (fresh sperm, freezing-thawing, capacitation, acrosome reaction) were separated, and stained by CBB, assayed using SDS-PAGE and Western blot analysis. The results showed that the expression level of sp32 was different in conversion of proacrosin/acrosin system after treatment of boar sperm capacitation, freezing-thawing and acrosome reaction, the expression of sp32 in experiment groups of capacitation and acrosome reaction were slightly higher than the freezing-thawing experiment group, and significantly higher than that of fresh semen group. The level of sp32 tyrosine phosphorylation had a significant difference between the freezing and thawing experiment group and other experiment groups. But bands with molecular mass of 38-170 ku in the fresh semen group were more obvious; it showed that the acrosomal membrane proteins were accompanied by modification and degradation of big molecular proteins when sperm underwent capacitation and the acrosome reaction. sp32 as a proacrosin binding protein, its expression and tyrosine phosphorylation level were up-regulated in the activation of the boar proacrosin/acrosin system.

CLC Number:

S828
S814.8

SUN Pei-liang, CUI Ming-xun, JIANG Yuan-yuan, CAO Li-peng, JIN Yi. The Expression and Tyrosine Phosphorylation of Sperm Protein 32 Regulate the Activation of the Boar Proacrosin/Acrosin System[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1229-1235.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.08.008

https://www.xmsyxb.com/EN/Y2013/V44/I8/1229

References

［1］TARDIF S, DUBÉ C, CHEVALIER S, et al. Capacitation is associated with tyrosine phosphorylation and tyrosine kinase-like activity of pig sperm proteins ［J］. Biol Reprod,2001,65:784-792.

［2］ARCELAY E, SALICIONI A M, WERTHEIMER E, et al. Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation ［J］. Int J Dev Biol,2008,52:463-472.

［3］TAKASHI W I, MAHBUB HASAN A K M, SATO K. Protein-tyrosine kinase signaling in the biological functions associated with sperm［J］. J Signal Transduct,2012,10(1):1-18.

［4］FICARRO S, CHERTIHIN O, WESTBROOK V A，et al. Phosphoproteome analysis of capacitated human sperm．Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation［J］．J Biol Chem,2003,278：11579-11589.

［5］HARAYAMA H, NISHIJIMA K, MURASE T, et al. Relationship of protein tyrosine phosphorylation state with tolerance to frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls［J］. Mol Reprod Dev,2010,77:910-921.

［6］HARRISON R, GAD ELLA B M. Bicarbonate induced membrane processing in sperm capacitation［J］.Theriogenology,2005,63:342-351.

［7］ARCELAY E, SALICIONIA M, WERTHEIMER E, et al. Identification of proteins undergoing tyrosine phosphorylation during mouse spermcapacitation［J］.Int J Dev Biol,2008,52:463-472.

［8］AMINARDE D E, FLAHERTY C. Sperm activation: role of reactive oxygen species and kinases［J］.Biochim Biophys Acta,2008,1784:106-115.

［9］BAILEY J L, TARDIF S, DUBÉ C, et al. Use of phosphoproteomics to study tyrosine kinase activity in capacitating boar sperm kinase activity and capacitation［J］. Theriogenology,2005,63:599-614.

［10］DUBÉ C, LECLERC P, BABA T, et al. The proacrosin binding protein,sp32,is tyrosine phosphorylated during capacitation of pig sperm［J］．J Androl,2005,26(4)：519-528.

［11］TARDIF S, DUBÉ C, BAILEY J L. Porcine sperm capacitation and tyrosine kinase acticity are dependent on bicarbonate and calcium but protein tyrosine phosphorylation is only associated with calcium［J］. Biol Reprod,2003,68:207-213.

［12］范蓉,罗彬,谢小薰,等.精子蛋白32研究进展［J］.解剖学报,2007,30(4):503-505.

［13］韩明铭,金一,方南洙,等.不同获能条件对猪精子体外获能和体外受精的影响［J］.中国畜牧杂志,2009,45(19):18-21.

［14］MOLLER C C, BLEIL J D, KINLOCH R A, et al. Structural and functional relationships between and hamster zonapellucida glycoprotein［J］. Dev Biol,1990,137(1):276-286.

［15］叶华虎,苟本富,刘卢生,等.考马斯亮蓝染色评价哺乳动物精子顶体反应发生的效果［J］.第三军医大学学报,2003,25(7):582-582,587.

［16］GONZÁLEZ-FERNÁNDEZ L, EGA-FERRUSOLA C O,MACIAS-GARCIA B,et al.Identification of protein tyrosine phosphatases and dual-specificity phosphatases in mammalian spermatozoa and their role in sperm motility and protein tyrosine phosphorylation［J］.Biol Reprod, 2009,80:1239-1252.

［17］张媛媛,胡启蒙,王亮亮,等.哺乳动物精子磷酸化蛋白质组学研究进展［J］.中国生物工程杂志,2012,32(4):76-82.

［18］李大吉.猪精子前顶体蛋白动力学活化作用的研究［D］.延吉：延边大学，2010.

［19］张旭成.不同处理对猪精子蛋白磷酸化的位点分布、水平及质量的影响［D］.延吉：延边大学，2010.

［20］KALAB P, PEKNICOVA J, GEUSSOVA G, et al. Regulation of protein tyrosine phosphorylation in boar sperm through a cAMP-dependent pathway［J］.Mol Reprod Dev,1998,51:304-314.

［21］BRAVO M M, APARICIO I M, GARCIA-HERRE-ROS M, et al. Changes in tyrosine phosphorylation associated with true capacitation and capacitation-like state in boar spermatozoa ［J］.Mol Reprod Dev,2005,71(1):88-96.

［22］BABA T, MCHIKAWAL Y, KASHIWABARA S, et al. Proacrosin activation in the presence of a 32 ku protein from boar spermatozoa ［J］. Biochem Biophys Res Commun,1989,160(3)：1026-1032.

［23］BABA T, NIIDA Y, MICHIKAWA Y, et al. An acrosomal protein, sp32, in mammalian sperm is a binding protein specific for two proacosins and an acrosin intermediate ［J］. J Biol Chem, 1994,269：10133-10140.

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	SHI Shengjie, WANG Liguang, GAO Lei, CAI Chuanjiang, HE Weixian, CHU Guiyan. Effect of miR-24-3p on Estradiol Synthesis in Porcine Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 169-178.
[4]	YANG Kai, LU Zhuoda, HE Jian, ZHANG Ruiqi, WANG Suqing, LI Kebiao, ZHAO Yunxiang, ZHU Xiaoping, GUO Jinbiao. The Population Effect of Commercial Pigs on the Accuracy of Genomic Selection for Carcass Traits in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4943-4951.
[5]	LI Chao, ZHAO Xueyan, WANG Yongjun, WANG Yanping, REN Yifan, LI Jingxuan, WANG Huaizhong, WANG Jiying, SONG Qinye. Analysis on the Structural Composition and Function of Bacterial Microbiota of Caecum and Colon in Laiwu and DLY pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5033-5045.
[6]	XU Tingting, QI Fenfang, HUANG Shihui, NIU Xi, LI Sheng, RAN Xueqin, WANG Jiafu, XIE Jian. Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5046-5055.
[7]	XUE Hongyan, YANG Mengyu, YANG Huan, DONG Lijun, CAI Xiaqing, ZHAO Zemin, WANG Xianzhong. The Role of ALOX15B-JNK in Heat Stress-induced Oxidative Stress and Apoptosis of Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5056-5065.
[8]	HU Ziping, WANG Ligang, ZONG Wencheng, HOU Renda, SU Yanfang, NIU Naiqi, WANG Lixian, WANG Yuan, ZHNAG Longchao. Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4117-4125.
[9]	JI Zhengyu, NI Mengru, ZHANG Zhaobo, ZHAO Gan, HUANG Zan, LI Pinghua, HUANG Ruihua, HOU Liming. Research on Adipogenic Capacity and Gene Expression Pattern of in Vitro FAPs Cells Derived from Longissimus Dorsi Muscle of Suhuai Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4126-4142.
[10]	BI Huan, QIN Hai, YUAN Wei, ZHANG Yudan, ZHANG Yiyu, CHEN Wei. Effect of Knocking down TYRP1 Gene on Melanin Production in the Xiang Pig Epidermal Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4143-4153.
[11]	LIU Pan, LI Ruiqi, TAN Zhankun, WANG Yifei, CHEN Xiaochen, HE Weixian, DU Renrang, MA Jian, CHU Guiyan, CAI Chuanjiang. Effects of High Fiber Diet on Growth Performance, Meat Quality and Intestinal Microbiome of Growing-finishing Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4247-4259.
[12]	LI Pinghui, PU Guang, WANG Zhongyu, ZHOU Wuduo, NIU Peipei, WU Chengwu, HOU Liming, HUANG Ruihua, LI Pinghua. Effect of Dietary Fiber Level on Blood and Intestinal Immune Indexes of Meishan Pigs and Preliminary Analysis of Its Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4260-4277.
[13]	WANG Hui, FENG Baoliang, WU Dan, XIANG Guangming, WANG Nan, MU Yulian, LI Kui, LIU Zhiguo. Research Progress of CD163 Gene and Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3127-3138.
[14]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[15]	LIAN Yuju, ZHANG Zhiyuan, LIAO Xiaobo, WEI Hongjiang, YIN Yulong, LIU Mei. Breeding Methods and Application Progress of Medical Miniature Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2667-2682.

The Expression and Tyrosine Phosphorylation of Sperm Protein 32 Regulate the Activation of the Boar Proacrosin/Acrosin System

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments