ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (12): 1867-1873.doi: 10.11843/j.issn.0366-6964.2013.12.002

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Advance of Capacitation Mechanism and the Physiochemical Changes of Sperm before and after Capacitation of Mammalian

HE Ya-nan1, ZHU Hua-bin1, CHEN Xiao-li1, HAO Hai-sheng1, QIN Tong1, ZHAO Xue-ming1, LU Yong-qiang2*, WANG Dong1*   

  1. (1.The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;2.Animal Husbandry and Veterinary Station of Beijing, Beijing 100107,China)
  • Received:2013-05-20 Online:2013-12-23 Published:2013-12-23

Abstract:

Before and after sperm capacitation, the motion trail and membrane microstructure were changed, and associated physiochemical change events of the metabolic types, enzyme activity and fluidity of related ions. In addition, we also reviewed the mechanism of capacitation from the cholesterol efflux, production of reactive oxygen species (ROX), protein tyrosine phosphorylation (PTP) and variation membrane potential. Meanwhile, the importance of PTP during sperm capacitation was pointed out in current review. We offered that the combined use of bioinformatics tools and proteomic/transcriptomics would be applicated on the study about cholesterol efflux from plasma membranes to acceptors mediated by the transporters, the effect of extracellular Ca2+ on capacitation, the inflow turn of extracellular Ca2+ and HCO3- accompanied with the cholesterol efflux and their relationship, as well as the PTP related to the signaling pathway, which could provide a theoretical guidance for further study on capacitation mechanism.

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