宁夏地区牛支原体、溶血性曼氏杆菌、多杀性巴氏杆菌和肺炎克雷伯菌感染监测与分析

doi:10.11843/j.issn.0366-6964.2025.11.030

Abstract

Abstract:

In recent years, bovine respiratory diseases have been increasingly reported, posing significant threats to the cattle industry. Large-scale outbreaks can result in substantial and often irreversible economic losses for livestock operations. This study aims to investigate the prevalence of key respiratory pathogens—Mycoplasma bovis (M. bovis), Mannheimia haemolytica (Mh), Pasteurella multocida (Pm), and Klebsiella pneumoniae (K.pn) —in calves from the Ningxia Hui Autonomous Region. A total of 457 nasal swab and tissue samples were collected from cattle across various counties and districts in Ningxia between 2022 and 2024. Pathogen identification was conducted using a combination of morphological examination and molecular diagnostic techniques. The infection rates of M. bovis, Mh, Pm, and K.pn were systematically analyzed based on year, geographic region, season, and developmental stage of the cattle. Among the four pathogens studied, Pm exhibited the highest overall detection rate (38.73%), a difference that was highly significant compared with the other three pathogens (P < 0.001). In the central arid zone, the prevalence of M. bovis was significantly greater than in both the Yellow River irrigation area (P < 0.01) and the southern mountainous region (P < 0.001). Seasonally, winter yielded peak detection rates for M. bovis (50%) and K.pn (70.5%), both of which exceeded summer and autumn rates (P < 0.001). Conversely, Pm reached its highest autumn prevalence (45.3%), significantly surpassing its winter (P < 0.01) and spring (P < 0.001) levels. During the growing phase, Pm detection was markedly higher than during both the lactation and mature phases (P < 0.001). Finally, single Pm infections were the most common (16.85%), significantly outnumbering mixed infections (P < 0.01), and the most frequent mixed co-infection occurred with Mh+Pm at 7.22%. This comprehensive epidemiological analysis delineates how temporal, geographical, seasonal, and developmental variables affect bovine respiratory pathogen prevalence in Ningxia, thereby furnishing a scientific framework to guide targeted prevention and control strategies for bacterial respiratory infections in the region.

Key words: Ningxia region, Mycoplasma bovis, Mannheimia hyopneumoniae, Pasteurella multocida, Klebsiella pneumoniae

CLC Number:

S858.235.1

LI Jiaren, GUO Ya'nan, WANG Jiandong, SHAO Xicheng, WANG Xueyi, HE Shenghu. Surveillance and Analysis of Mycoplasma bovis, Mannheimia hyopneumoniae, Pasteurella multocida and Klebsiella pneumonia in Ningxia Region[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5721-5731.

Figures/Tables 8

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References 24

1	邢小勇,程家海,包世俊,等.牛支原体、肺炎克雷伯菌与牛传染性鼻气管炎病毒多重PCR方法的建立与初步应用[J].农业生物技术学报,2021,29(5):1024-1030.
	XINGX Y,CHENGJ H,BAOS J,et al.Establishment and preliminary application of multiplex PCR for Mycoplasma bovis, Klebsiella pneumoniae and bovine infectious rhinotracheitis virus[J].Journal of Agricultural Biotechnology,2021,29(5):10.
2	周玉龙,吴丹丹,周金玲,等.牛呼吸道综合症多重PCR检测方法的建立[J].黑龙江八一农垦大学学报,2016,28(6):97-101, 107.
	ZHOUY L,WUD D,ZHOUJ L,et al.Establishment of multiple PCR detection method of bovine respiratory disease complex[J].Journal of Heilongjiang Bayi Agricultural University,2016,28(6):97-101, 107.
3	张学虎,赵兴绪,张勇,等.一例牛支原体与溶血性曼氏杆菌混合感染的诊断与治疗[J].黑龙江畜牧兽医,2023(16):83-87, 134-135.
	ZHANGX H,ZHAOX X,ZHANGY,et al.Diagnosis and treatment of a case of mixed infection with Mycoplasma bovis and Mannheimia haemolytica[J].Heilongjiang Animal Science and Veterinary Medicine,2023(16):83-87, 134-135.
4	薛念宇,李中元,倪宏波.牛多杀性巴氏杆菌和肺炎克雷伯氏菌混合感染的实验室诊断[J].现代畜牧兽医,2020(2):41-44.
	XUEN Y,LIZ Y,NIH B,et al.Laboratory diagnosis of mixed infection of Pasteurella and Klebsiella in cattle[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2020(2):41-44.
5	蔺俐仲,邓珊珊,张海,等.一例牛支原体与巴氏杆菌混合感染的诊治[J].贵州畜牧兽医,2020,44(3):52-54.
	LINL Z,DENS S,ZHANGH,et al.Diagnosis and treatment of a mixed infection of Mycoplasma bovis and Pasteurella mutocida[J].Guizhou Journal of Animal Husbandry & Veterinary Medicine,2020,44(3):52-54.
6	孙凌炜. 中国北方部分地区牛呼吸道综合征主要病原流行病学调查[D]. 呼和浩特: 内蒙古农业大学, 2021.
	SUN L W. Epidemiological Survey of the main pathogens of bovine respiratory syndrome in parts of Northern China[D]. Hohhot: Inner Mongolia Agricultural University, 2021. (in Chinese)
7	李百云,许泽华,郭鑫年,等.宁夏不同生态类型区土壤养分状况比较分析[J].寒旱农业科学,2024,3(5):434-440.
	LIB Y,XUZ H,GUOX N,et al.Comparative Analysis of soil nutrient status across different ecological zones in Ningxia[J].Journal of Cold-Arid Agricultural Sciences,2024,3(5):434-440.
8	岳康,王建东,唐玉林,等.宁夏主要肉牛养殖地区肉牛繁殖状况[J].上海畜牧兽医通讯,2022(3):43-46.
	YUEK,WANGJ D,TANGY L,et al.Reproductive status of beef cattle in major cattle-raising areas of Ningxia[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2022(3):43-46.
9	DUBROVSKYS A,VAN EENENNAAMA L,KARLEB M,et al.Epidemiology of bovine respiratory disease (BRD) in preweaned calves on California dairies: The BRD 10K study[J].J Dairy Sci,2019,102(8):7306-7319. doi: 10.3168/jds.2018-14774
10	CALLANR J,GARRYF B.Biosecurity and bovine respiratory disease[J].Vet Clin North Am Food Anim Pract,2002,18(1):57-77. doi: 10.1016/S0749-0720(02)00004-X
11	TOWNSENDK M,FROSTA J,LEEC W,et al.Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates[J].J Clin Microbiol,1998,36(4):1096-1100. doi: 10.1128/JCM.36.4.1096-1100.1998
12	张晓宇. 奶牛犊牛主要呼吸道疾病流行病学调查及牛支体肺炎防治的研究[D]. 呼和浩特: 内蒙古农业大学, 2018.
	ZHANG X Y. Investigation on pathogens of main respiratory infectious diseases in dairy cows in China and research on main pathogen control[D]. Inner Mongolia Agricultural University, 2018. (in Chinese)
13	张金花,郭亚男,王建东,等.绵羊肺炎支原体和多杀性巴氏杆菌混合感染的诊断、病理组织学观察和耐药性分析[J].畜牧与兽医,2022,54(11):72-79.
	ZHANGJ H,GUOY N,WANGJ D,et al.Diagnosis, histopathological observation and drug resistance analysis of mixed infection of Mycoplasma ovipneumoniae and Pasteurella multocida[J].Animal Husbandry & Veterinary Medicine,2022,54(11):72-79.
14	李聚才,杨正义,张生,等.宁夏中部干旱带肉牛产业发展现状、问题与对策——以同心县为例[J].宁夏农林科技,2012,53(1):96-100.
	LIJ C,YANGZ Y,ZHANGS,et al.Developing status, problems and countermeas ure of beef cattle industry from the middle Part Arid Zone in Ningxia——Taking Tongxin Countyas an example[J].Ningxia Journal of Agriculture and Forestry Science and Tec hnology,2012,53(1):96-100.
15	刘汝亮,洪瑜,李友宏,等.推进农业绿色发展, 助推宁夏引黄灌区乡村振兴[J].宁夏农林科技,2019,60(9):75-77.
	LIUR L,HONGY,LIY H,et al.Promoting the green development of agriculture to boost rural revitalization of Ningxia Yellow River irrigation area[J].Ningxia Journal of Agriculture and Forestry Science and Technology,2019,60(9):75-77.
16	郭亚男,闫背背,李勇,等.2020—2021年宁夏地区牛支原体血清流行病学调查与分析[J].黑龙江畜牧兽医,2023(21):72-77, 83.
	GUOY N,YANB B,LIY,et al.Seroepidemiological investigation and analysis of Mycoplasma bovis in Ningxia region from 2020 to 2021[J].Heilongjiang Animal Science and Veterinary Medicine,2023(21):72-77, 83.
17	CALDERÓN BERNALJ M,FERNáNDEZA,ARNALJ L,et al.Cluster analysis of bovine respiratory disease (BRD)-associated pathogens shows the existence of two epidemiological patterns in BRD outbreaks[J].Vet Microbiol,2023,280,109701. doi: 10.1016/j.vetmic.2023.109701
18	MARTINS W,SCHWABEC W,FRANTIC E.Dairy calf mortality rate: influence of management and housing factors on calf mortality rate in Tulare County, California[J].Am J Vet Res,1975,36(8):1111-1114. doi: 10.2460/ajvr.1975.36.08.1111
19	哈力木别克·马勒哈买提.季节性气候变化对牛疾病的影响[J].养殖与饲料,2018(2):48-49.
	HALIMUBIEKE·M.Effects of seasonal climate change on bovine diseases[J].Animals Breeding and Feed,2018(2):48-49.
20	袁敏明.牦牛巴氏杆菌病流行病学调查报告[J].农家参谋,2022(16):129-131.
	YUANM M.Epidemiological investigation report on Pasteurellosis in yaks[J].The Farmers Consultant,2022(16):129-131.
21	李媛,闫磊,刘桐,等.中国西部和北部边境4省区牛支原体血清流行病学调查及分析[J].中国预防兽医学报,2021,43(7):717-721.
	LIY,YANL,LIUT,et al.Seroprevalence and analysis of Mycoplasma bovis infection in four provinces of western and northern China[J].Chinese Journal of Preventive Veterinary Medicine,2021,43(7):717-721.
22	佘海瑞.牦牛犊牛溶血性曼氏杆菌与多杀性巴氏杆菌混合感染的病原分离鉴定及药敏实验[J].畜牧兽医杂志,2021,40(4):151-154.
	SHEH R.Isolation, identification and drug sensitivity test of pathogens of mixed infection of Mannheimia haemolytica and Pasteurella multocida in yak calves[J].Journal of Animal Science and Veterinary Medicine,2021,40(4):151-154.
23	LACHOWICZ-WOLAKA,KLIMOWI CZ-B ODYSMD,PŁONECZKA-JANECZKOK,et al.The prevalence, coexistence, and correlations between seven pathogens detected by a PCR method from South-Western Poland dairy cattle suffering from bovine respiratory disease[J].Microorganisms,2022,10(8):1487. doi: 10.3390/microorganisms10081487
24	HOUGHTONS B,GOURLAYR N.Synergism between Mycoplasma bovis and Pasteurella haemolytica in calf pneumonia[J].Vet Rec,1983,113(2):41-42. doi: 10.1136/vr.113.2.41

分组依据 Grouping basis	组别 Group	采集样本数/份 Number of collected samples
区域 Area	引黄灌区Yellow river irrigation district	282
	中部干旱带The central arid belt	48
	南部山区Southern mountainous area	127
年份 Year	2022年2022	261
	2023年2023	56
	2024年2024	140
季节 Season	春季Spring	106
	夏季Summer	243
	秋季Autumn	64
	冬季Winter	44
年龄阶段 Age stage	哺乳期Lactation period	299
	育成期Rearing period	130
	成熟期Maturity period	28

病原菌 Pathogenic bacteria	目的基因 Target genes	引物序列(5′→ 3′) Primer sequences	产物大小/bp Product size	退火温度/℃ Annealing temperature
多杀性巴氏杆菌 Pm	kmt 1	F: ATC CGC TAT TTA CCC AGT G	456	56
多杀性巴氏杆菌 Pm	kmt 1	R: GCT GTA AAC GAA CTC GCC	456	56
溶血性曼氏杆菌 Mh	gcp	F: TGG GCA ATA CGA ACT ACG GG	227	55
溶血性曼氏杆菌 Mh	gcp	R: CTT TAA TCG TAT TCG ACA GC	227	55
肺炎克雷伯菌 K.pn	Khe	F: TTC ATC TAC GTG CTG GAG GG	192	56
肺炎克雷伯菌 K.pn	Khe	R: CGG GTA ATA AAT GCG GTT GT	192	56
牛支原体 M. bovis	uvrC	F: GAATTCAATGTGTCTACTAGTC-CTGG	1 600	50
牛支原体 M. bovis	uvrC	R: AAGCTTAGCGT-CATAGATTTTTGCATA	1 600	50

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Surveillance and Analysis of Mycoplasma bovis, Mannheimia hyopneumoniae, Pasteurella multocida and Klebsiella pneumonia in Ningxia Region

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