基于scRNA-seq解析猪圆形精子的动态转录差异

doi:10.11843/j.issn.0366-6964.2025.11.016

Abstract

Abstract:

In order to investigate the dynamic transcriptional differences of round spermatids, the present study was conducted on adult Guanzhong black pigs (10-12 months), based on 10×single cell RNA sequencing (scRNA-seq) data from testicular tissues, to analyze germ cell clustering and round spermatids reclustering, and to functionally annotate and enrich differentially expressed genes in round spermatids, with a view to investigating the developmental changes of round spermatozoa. We analyzed germ cell clustering and round spermatids reclustering, as well as functional annotation and enrichment of differentially expressed genes in round spermatids, in order to investigate the developmental changes of round spermatids. The results identified 5 porcine round spermatids types, mapped the cellular trajectories of early round spermatids from development to the spermatid stage, and found that the differentially expressed genes in round spermatids and spermatozoa were related to the process of protein binding and endoplasmic reticulum activity, mainly enriched in pathways such as spermatid development through GO and KEGG enrichment analyses; histone variant analyses showed that the fischerin family and the convertin family might also play an important role in the development of porcine round spermatids. Candidate genes were verified by immunohistochemical assays. Based on the results, it was hypothesized that SUN5, SPATA3, and APOC4 could be used as regulatory genes for round spermatids development. This study can provide theoretical basis for the subsequent injection of round spermatozoa into the cytoplasm of the egg by biotechnology, and also provide reference information for porcine round spermatids monocytomics.

Key words: round spermatids, scRNA-seq, porcine

CLC Number:

S828.3

MA Yuan, JIN Haoyan, WANG Nana, XIE Yaru, LI Tianjiao, ZHANG Lingkai. Dynamic Transcriptional Differences in Porcine Round Spermatids Resolved Based on scRNA-seq[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5545-5562.

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簇Clusters	上调基因数量/个Number of up-regulated genes	簇Clusters	上调基因数量/个Number of up-regulated genes
0	660	9	1 020
1	1 485	10	1 201
2	1 821	11	1 882
3	165	12	1 782
4	1 526	13	233
5	747	14	1 397
6	1 345	15	1 096
7	1 205	16	1 575
8	1 002	-	-

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Dynamic Transcriptional Differences in Porcine Round Spermatids Resolved Based on scRNA-seq

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