Table 1

Composition of experimental diets"

Table 2

Nutritional content of experimental diets  %"

Fig. 1

Morphological comparison of adipocytes and comparison of differences in adipocyte diameter in back fat between Jinwu pigs and DLY pigs A. Jinwu pigs and DLY pigs back fat hematoxylin-eosin staining; B. Comparison of adipocyte diameter, " ***"means extremely significant difference(P < 0.01)"

Fig. 2

Comparison of subclass lipid content in the subcutaneous fat tissue of the back between Jinwu pigs and DLY pigs"

Fig. 3

Comparison of lipid content detected in Jinwu pigs and DLY pigs in both positive and negative ion modes A. Comparison of lipid content in the subcutaneous fat tissue of the back between Jinwu pigs and DLY pigs in positive ion mode; B. Comparison of lipid content in the subcutaneous fat tissue of the back between Jinwu pigs and DLY pigs in negative ion mode"

Fig. 4

Multivariate analysis score comparison between Jinwu pigs and DLY pigs A. PCA results, blue represent Jinwu pigs, while red represent DLY pigs; B. PLS-DA results, red circle represent Jinwu pigs, and blue circle represent DLY pigs"

Fig. 5

Differential lipid molecules between Jinwu pigs and DLY pigs were analyzed using their screening process (Venn diagram), distribution (volcano plot), and clustering pattern (heatmap) A. Venn diagram of 3 screening methods (FC > 1.5 or FC < 0.65, P < 0.05 and VIP > 1); B. Volcano plot of differential lipids, red color represent up regulated lipids, while blue color represent down regulated lipids; C. Heatmap of differential lipids clustering, red color represent up regulated lipids, while blue color represent down regulated lipids"

Table 3

The index of differential lipids between Jinwu pigs and DLY pigs (the top 20 lipids)"

Fig. 6

Differential lipids structure and functional enrichment analysis of Jinwu pigs and DLY pigs A. The differential lipid structure feature chart shows the number of carbon double bonds on the x-axis and the number of carbon atoms in lipids on the y-axis. Red indicates up regulated lipids, while blue indicates down regulated lipids. "○" represents P < 0.05, "△" represents P < 0.01, and "*" represents P < 0.001. B. Functional enrichment analysis of differential lipids was performed using the LION lipid ontology database"

Fig. 7

Correlation analysis among differential lipids Red color indicates a positive correlation, and blue color indicates a negative correlation. "*"indicates 0.01 < P < 0.05, " **" indicates 0.001 < P < 0.01, " ***" indicates P≤0.001"

Fig. 8

Correlation analysis between differential lipids and adipocyte diameter Red lines represent up regulated lipids, while blue lines represent down regulated lipids. The thickness of the connecting lines indicates the strength of the correlation, with thicker lines representing stronger correlations"