Table 1

Main materials and instrument details"

Fig. 1

GSH alleviates the decrease in PK-15 cell activity caused by CdCl2 A. Safe concentration screening of GSH; B. The effect of GSH on the vaibility of PK-15 cells treated with CdCl2. **. P < 0.01; ***. P < 0.001; ns. P>0.05"

Fig. 2

The effect of GSH on CdCl2 induced morphological damage in PK-15 cells"

Fig. 3

The effect of GSH on CdCl2 induced ROS elevation in PK-15 cells A. ROS variation of HK-2 cells after CdCl2 treatment observed by fluorescence microscope; B. ROS level of HK-2 cells after CdCl2 treatment detected by flow cytometry. **. P < 0.01"

Fig. 4

The effect of GSH on CdCl2 induced mitochondrial damage in PK-15 cells A. Changes of JC-1 staining in cells of different treatment groups using fluorescence microscopy; B. Flow cytometry detection of mitochondrial membrane potential (Δψm) in cells of different treatment groups. **. P < 0.01"

Fig. 5

CdCl2 induced DNA damage in PK-15 cells A. DNA damage of PK-15 cells detected by comet assay; B. Percentage of tail DNA; C. Tail length; D. γ-H2AX foci detected by immunofluorescence staining. **. P < 0.01"

Fig. 6

The effect of GSH on CdCl2 induced apoptosis of PK-15 cells A. Hoechst 33342 nuclear staining showed the appearance of chromatin condensation (arrow head); B. Detection of apoptosis rate of cells in different treatment groups by flow cytometry; C. Bax, Bcl-2 and cleaved caspase-3 were detected by Western blot. **. P < 0.01"