基于全基因组重测序分析道州灰鹅保种效果

doi:10.11843/j.issn.0366-6964.2025.02.015

Abstract

Abstract:

This study aimed to investigate the genetic structure, diversity, and kinship of geese at the Daozhou gray goose conservation farm to guide future conservation efforts.The Daozhou gray goose conservation farm used a single-sire family rotation model, involving 49 families. A healthy 200-day-old male goose from each family was selected for DNA extraction via venous blood sampling. The DNA samples were subjected to whole-genome resequencing, and the resulting data were analyzed using bioinformatics software to assess the genetic structure and diversity of the population.A total of 15 245 050 SNPs were detected in the Daozhou gray goose conservation population, reducing to 14 628 485 after quality control. Genetic diversity analysis revealed an average observed heterozygosity (Ho) of 0.280, expected heterozygosity (He) of 0.288, inbreeding coefficient (Fis) of 0.070, average polymorphism information content (PIC) of 0.262, and gene diversity index (Nei) of 0.331. A total of 802 runs of homozygosity (ROH) were identified, with an inbreeding coefficient of 0.010, indicating low inbreeding levels. IBS distance matrix and G-matrix results showed that only a few individuals had close kinship. Population admixture and principal component analysis indicated that most individuals clustered into one subpopulation, while a few formed two small subpopulations. Fst calculation result showed moderate differentiation between the two smaller subpopulations.In summary, the conserved population of Daozhou gray goose exhibits relatively rich genetic diversity and a low level of inbreeding, reflecting good conservation outcomes. It is recommended to decrease breeding between family lines with excessively close kinship relationships in order to maintain genetic diversity and ensure the long-term health of the population.

Key words: resequencing, Daozhou gray goose, genetic structure

CLC Number:

S835.2

WAN Weican, HE Xu, LIU Yang, MA Yuyong, JIANG Yuzhang, DAI Qiuzhong, YAN Haifeng, JIANG Guitao, LI Chuang. Evaluation of Daozhou Gray Goose Conservation Based on Whole Genome Resequencing Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(2): 633-642.

Figures/Tables 7

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遗传多样性指标 Genetic diversity index	均值±标准差 Mean±SD
观测杂合度Ho	0.280±0.004
期望杂合度He	0.288±0.026
群体近亲系数Fis	0.070±0.050
多态信息含量PIC	0.262±0.092
基因多样性指数Nei	0.331±0.193

分群 Group	亚群1 Subgroup 1	亚群2 Subgroup 2	亚群3 Subgroup 3
亚群1 Subgroup 1	/	0.037 4	0.100 8
亚群2 Subgroup 2	/	/	0.026 7

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Evaluation of Daozhou Gray Goose Conservation Based on Whole Genome Resequencing Analysis

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