基因型特征提取方法影响基因组选择预测准确性的研究

doi:10.11843/j.issn.0366-6964.2024.06.015

Abstract

Abstract:

The purpose of this study was to explore and evaluate 6 different methods for extracting genotype feature of single nucleotide polymorphisms (SNP). Six methods were analyzed and compared: principal component analysis (PCA), gene-principal component analysis (gene-PCA), SNP-Pearson correlation coefficient (SNP-PCC), linkage disequilibrium (LD), and genome-wide association study (GWAS) and random sampling (RS). The prediction accuracy of GEBV in 2 sets of data (Beijing duck, 542 samples, SNP loci 39 932; Duroc pig, 2 549 samples, SNP loci 230 884) and 3 sets of phenotypes (Beijing duck body length, Duroc pig backfat thickness and teat number) was evaluated. Results showed that SNP-PCC combined with 5 GS methods (GBLUP, BayesA, BayesB, BayesC, and Bayesian Lasso) achieved relatively reliable prediction accuracy for the Pecking duck body length phenotype and achieved the highest average prediction accuracy in pig backfat thickness and teat number phenotypes (increased by 5%, reaching 32.3%), and significantly improved computational efficiency (on average 5-7 times faster). In summary, this study found that selecting appropriate feature extraction methods can effectively improve the accuracy and computational efficiency of GS prediction, laying the foundation for in-depth research on the impact of different feature extraction methods on GS prediction accuracy, and providing reference for their application in breeding practice.

Key words: genomic selection, feature extraction, prediction accuracy

CLC Number:

S813.1

Huaxuan WU, Zhiqiang DU. Methods of Genotype Feature Extraction Affecting the Prediction Accuracy of Genomic Selection[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2431-2440.

Figures/Tables 7

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References 41

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表型 Phenotype	样本数 Number of samples	平均值 Mean	标准差 Standard deviation	最大值 Max value	最小值 Min value
体长/cm Body length	542	51.30	2.70	58.00	42.00
背膘厚/mm Backfat thickness	2 549	10.91	2.06	19.70	5.48
乳头数/个Teat number	2 549	10.72	1.06	15.00	8.00

数据集Dataset	SNPs	GWAS(P < 0.05)	PCA	Gene-PCA	LD	SNP-PCC	RS
体长Body length	39 932	4 282	359	542	31 002	26 495	3 993
背膘厚、乳头数 Backfat thickness, teat number	230 884	21 760	716	2 500	33 101	20 731	23 088

数据集 Dataset	方法 Method	平均PCC Mean PCC	PCC标准差 PCC STD	平均MSE Mean MSE	MSE标准差 MSE STD
北京鸭，体长/cm	GWAS(P < 0.05)	0.484	0.081	8.701	2.835
Pecking duck, body length	PCA	0.243	0.043	5.741	0.244
	Gene-PCA	0.132	0.026	6.094	0.080
	LD	0.277	0.052	5.803	0.343
	SNP-PCC	0.290	0.058	5.916	0.556
	Random	0.265	0.036	6.002	0.375
	Origin	0.217	0.020	6.525	0.888
猪，背膘厚/mm	GWAS(P < 0.05)	0.366	0.037	4.927	0.595
Pig, backfat thickness	PCA	0.341	0.034	4.102	0.105
	Gene-PCA	0.186	0.025	4.560	0.118
	LD	0.358	0.020	4.259	0.211
	SNP-PCC	0.367	0.023	4.164	0.181
	Random	0.338	0.021	4.286	0.158
	Origin	0.336	0.026	4.612	0.552
猪，乳头数/个	GWAS(P < 0.05)	0.240	0.042	1.247	0.105
Pig, teat number	PCA	0.313	0.030	1.024	0.029
	Gene-PCA	0.123	0.011	1.148	0.023
	LD	0.301	0.022	1.111	0.068
	SNP-PCC	0.312	0.018	1.090	0.043
	Random	0.299	0.020	1.088	0.053
	Origin	0.280	0.025	1.207	0.158

数据集 Dataset	方法 Method	位点数 Number of markers	计算速度 Computing speed
体长	GWAS(P < 0.05)	4 282	2 s
Body length	PCA	359	1 s
	Gene-PCA	542	1 s
	LD	31 002	10 s
	SNP-PCC	26 495	6 s
	Random	3 993	2 s
	Origin	39 932	15 s
背膘厚、乳头数	GWAS(P < 0.05)	21 760	12 s
Backfat thickness, teat number	PCA	716	1 s
	Gene-PCA	2 500	1.6 s
	LD	33 101	18 s
	SNP-PCC	20 731	11 s
	Random	23 088	12 s
	Origin	230 884	2 min 20 s

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Methods of Genotype Feature Extraction Affecting the Prediction Accuracy of Genomic Selection

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