Enolase在鸭疫里默氏杆菌侵袭鸭脑微血管内皮细胞中的作用

doi:10.11843/j.issn.0366-6964.2022.12.024

Abstract

Abstract: This study aimed to clarify the role of Enolase on Riemerella anatipestifer invading duck brain microvascular endothelial cells (DBMEC) and blood brain barrier. R. anatipestifer enolase gene deletion mutant ΔEnolase and the complemented strain cΔEnolase were constructed by homologous recombination and combined transfer assay using RA-LZ01 strain as the parental strain. The differences of adherence and invasion abilities of RA-LZ01, ΔEnolase and cΔEnolase to DBMEC were detected. At the same time, bacterial loads in blood and brain of ducklings infected with RA-LZ01, ΔEnolase and cΔEnolase were determined respectively.The results showed that, compared with RA-LZ01, the adhesion and invasion rates of ΔEnolase to DBMEC were significantly reduced, and cΔEnolase restored the adhesion and invasion abilities to DBMEC. Bacterial loads in blood had no significant difference among the animals infected with RA-LZ01, ΔEnolase or cΔEnolase. Compared with the ducklings infected with RA-LZ01 and cΔEnolase strains, the loads of bacteria in the brain of the ducklings infected with ΔEnolase strain was significantly decreased. These results indicated that Enolase was significantly correlated with the adhesion and invasion of R. anatipestifer into DBMEC and the invasion of ducklings’ brain. Hence, Enolase should be a virulence factor mediating R. anatipestifer crossing duck blood brain barrier.

Key words: Riemerella anatipestifer, Enolase, duck brain microvascular endothelial cells, blood brain barrier

CLC Number:

S858.325.12

ZOU Ronghua, WU Xiaoni, CHEN Qiwei, GONG Xiaowei, WANG Yanping, ZHENG Fuying, CHU Yuefeng. Effect of Enolase on Riemerella anatipestifer Invading Duck Brain Microvascular Endothelial Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4389-4397.

References 27

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Effect of Enolase on Riemerella anatipestifer Invading Duck Brain Microvascular Endothelial Cells

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