METTL16在鸡不同类型肌肉中的表达规律及其对肌肉功能的调控作用

doi:10.11843/j.issn.0366-6964.2023.02.012

Abstract

Abstract: The purpose of this study was to explore the expression of m6A methyltransferase-like protein 16 (METTL16) in different types of chicken muscle tissue and its regulatory role in chicken skeletal muscle function. In the present study, the tissue expression profile of METTL16 in different muscles of 120-day-old Guangxi partridge hens and the expression of genes related to proliferation, differentiation and muscle fiber formation after siRNA interference by qRT-PCR were analyzed. The results showed that METTL16 gene was widely expressed in different muscle tissues of chickens. White muscles, including major pectoralis, minor pectoralis and lateral iliotibial muscles, have higher METTL16 gene expression. Cell proliferation assay showed that the proliferation activity of chicken myoblasts was inhibited after METTL16 interference. Interfering with METTL16 gene during myoblast differentiation could reduced the methylation level of m6A and slow-MyHC expression in chicken myoblasts, significantly reduced MyoD expression (P<0.05), and significantly elevated fast-MyHC expression (P<0.001). In conclusion, the expression of METTL16 gene in different muscles is related to the composition of muscle fiber types. METTL16 gene may play a significant role in chicken myoblast proliferation and differentiation, and inhibit the formation of fast-twitch muscle fibers. These results indicated that METTL16 might play a crucial role in chicken muscle function regulation.

Key words: chicken, myofiber, myoblast, proliferation and differentiation, METTL16 gene

CLC Number:

S831.2

PANG Lichuan, SHAN Yanju, LIU Yifan, ZHANG Ming, GAN Dafeng, TU Yunjie, JI Gaige, JU Xiaojun, SHU Jingting, ZOU Jianmin. Expression of METTL16 in Different Types of Chicken Muscle and Its Regulatory Role in Chicken Skeletal Muscle Function[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 545-553.

References 32

[1]	FRONTERA W R, OCHALA J.Skeletal muscle:A brief review of structure and function[J].Calcif Tissue Int, 2015, 96(3):183-195.
[2]	MEUNIER B, PICARD B, ASTRUC T, et al.Development of image analysis tool for the classification of muscle fibre type using immunohistochemical staining[J].Histochem Cell Biol, 2010, 134(3):307-317.
[3]	LISTRAT A, LEBRET B, LOUVEAU I, et al.How muscle structure and composition influence meat and flesh quality[J].Sci World J, 2016, 2016:3182746.
[4]	DESROSIERS R, FRIDERICI K, ROTTMAN F.Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells[J].Proc Natl Acad Sci U S A, 1974, 71(10):3971-3975.
[5]	ROIGNANT J Y, SOLLER M.m6A in mRNA:an ancient mechanism for fine-tuning gene expression[J].Trends Genet, 2017, 33(6):380-390.
[6]	PAN Y T, MA P, LIU Y, et al.Multiple functions of m6A RNA methylation in cancer[J].J Hematol Oncol, 2018, 11(1):48.
[7]	NIU Y M, ZHAO X, WU Y S, et al.N6-methyl-adenosine (m6A) in RNA:an old modification with a novel epigenetic function[J]. Genomics Proteomics Bioinformatics, 2013, 11(1):8-17.
[8]	HUANG H L, WENG H Y, CHEN J J. m6A modification in coding and non-coding RNAs:roles and therapeutic implications in cancer[J].Cancer Cell, 2020, 37(3):270-288.
[9]	LIU J Z, YUE Y N, HAN D L, et al.A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation[J].Nat Chem Biol, 2014, 10(2):93-95.
[10]	FU Y, DOMINISSINI D, RECHAVI G, et al.Gene expression regulation mediated through reversible m6A RNA methylation[J]. Nat Rev Genet, 2014, 15(5):293-306.
[11]	WANG X, FENG J, XUE Y, et al.Structural basis of N6-adenosine methylation by the METTL3-METTL14 complex[J].Nature, 2016, 534(7608):575-578.
[12]	盛杰.METTL3通过Wnt信号通路调控肌肉分化的机制研究[D].武汉:武汉科技大学, 2021.SHENG J.METTL3 regulates the mechanism of muscle differentiation through Wnt signaling pathway[D].Wuhan:Wuhan University of Science and Technology, 2021.(in Chinese)
[13]	XIE S J, LEI H, YANG B, et al.Dynamic m6A mRNA methylation reveals the role of METTL3/14-m6A-MNK2-ERK signaling axis in skeletal muscle differentiation and regeneration[J].Front Cell Dev Biol, 2021, 9:744171.
[14]	DENG K P, FAN Y X, LIANG Y X, et al.FTO-mediated demethylation of GADD45B promotes myogenesis through the activation of p38 MAPK pathway[J].Mol Ther Nucleic Acids, 2021, 26:34-48.
[15]	束婧婷, 单艳菊, 姬改革, 等.广西麻鸡m6A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J].中国农业科学, 2022, 55(3):589-601.SHU J T, SHAN Y J, JI G G, et al.Relationship between expression levels of Guangxi partridge chicken m6A methyltransferase genes, myofiber types and myogenic differentiation[J].Scientia Agricultura Sinica, 2022, 55(3):589-601.(in Chinese)
[16]	AOYAMA T, YAMASHITA S, TOMITA K.Mechanistic insights into m6A modification of U6 snRNA by human METTL16[J]. Nucleic Acids Res, 2020, 48(9):5157-5168.
[17]	MENDEL M, CHEN K M, HOMOLKA D, et al.Methylation of structured RNA by the m6A writer METTL16 is essential for mouse embryonic development[J].Mol Cell, 2018, 71(6):986-1000.e11.
[18]	PENDLETON K E, CHEN B B, LIU K Q, et al.The U6 snRNA m6A methyltransferase METTL16 regulates SAM synthetase intron retention[J].Cell, 2017, 169(5):824-835.e14.
[19]	WARDA A S, KRETSCHMER J, HACKERT P, et al.Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs[J].EMBO Rep, 2017, 18(11):2004-2014.
[20]	SATTERWHITE E R, MANSFIELD K D.RNA methyltransferase METTL16:targets and function[J].Wiley Interdiscip Rev RNA, 2022, 13(2):e1681.
[21]	DOXTADER K A, WANG P, SCARBOROUGH A M, et al.Structural basis for regulation of METTL16, an S-adenosylmethionine homeostasis factor[J].Mol Cell, 2018, 71(6):1001-1011.e4.
[22]	SHIMA H, MATSUMOTO M, ISHIGAMI Y, et al.S-adenosylmethionine synthesis is regulated by selective N6-adenosine methylation and mRNA degradation involving METTL16 and YTHDC1[J].Cell Rep, 2017, 21(12):3354-3363.
[23]	SU R, DONG L, LI Y C, et al.METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis[J].Nat Cell Biol, 2022, 24(2):205-216.
[24]	KOVAČŘÍKOVÁ A S, STIXOVÁ L, KOVAČŘÍK A, et al.N6-adenosine methylation in RNA and a reduced m3G/TMG level in non-coding RNAs appear at microirradiation-Induced DNA lesions[J].Cells, 2020, 9(2):360.
[25]	丁浩, 林月月, 张涛, 等.m6A甲基化在鸡肌肉生长发育中的表达研究[J].中国畜牧兽医, 2021, 48(5):1525-1534.DING H, LIN Y Y, ZHANG T, et al.Study on the expression of m6A methylation in chicken muscle growth and development[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(5):1525-1534.(in Chinese)
[26]	WEINTRAUB H, DAVIS R, TAPSCOTT S, et al.The myoD gene family:nodal point during specification of the muscle cell lineage[J].Science, 1991, 251(4995):761-766.
[27]	HERNÁNDEZ-HERNÁNDEZ J M, GARCÍA-GONZÁLEZ E G, BRUN C E, et al.The myogenic regulatory factors, determinants of muscle development, cell identity and regeneration[J].Semin Cell Dev Biol, 2017, 72:10-18.
[28]	ASFOUR H A, ALLOUH M Z, SAID R S.Myogenic regulatory factors:the orchestrators of myogenesis after 30 years of discovery[J].Exp Biol Med (Maywood), 2018, 243(2):118-128.
[29]	贾安峰, 冯京海, 张敏红.调控骨骼肌肌纤维类型转化的因素及机制[J].动物营养学报, 2014, 26(5):1151-1156.JIA A F, FENG J H, ZHANG M H.Factors and mechanisms in regulating transition of skeletal muscle fiber types[J].Chinese Journal of Animal Nutrition, 2014, 26(5):1151-1156.(in Chinese)
[30]	SCHIAFFINO S, REGGIANI C.Fiber types in mammalian skeletal muscles[J].Physiol Rev, 2011, 91(4):1447-1531.
[31]	KUDOU K, KOMATSU T, NOGAMI J, et al.The requirement of Mettl3-promoted MyoD mRNA maintenance in proliferative myoblasts for skeletal muscle differentiation[J].Open Biol, 2017, 7(9):170119.
[32]	WANG X K, ZHANG Y W, WANG C M, et al.METTL16 promotes cell proliferation by up-regulating cyclin D1 expression in gastric cancer[J].J Cell Mol Med, 2021, 25(14):6602-6617.

Expression of METTL16 in Different Types of Chicken Muscle and Its Regulatory Role in Chicken Skeletal Muscle Function

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 32

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Jiajia, XU Dan, LIU Yang, ZHAO Xiaoling. Research Progress on Genetic Regulation Mechanism of Barring Feather Trait in Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1883-1892.
[2]	LEI Yanru, HU Xiaoyu, XU Chunhong, ZHANG Chenxi, DU Wenping, WANG Yangguang, LI Donghua, SUN Guirong, LI Wenting, KANG Xiangtao. Comparative Analysis of Growth, Carcass and Meat Quality Traits of Five Hybrid Combinations of Houdan Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1521-1535.
[3]	CAO Yuzhu, XING Yuxin, MA Chenglin, GUAN Hongbo, JIA Qihui, KANG Xiangtao, TIAN Yadong, LI Zhuanjian, LIU Xiaojun, LI Hong. Biological Characterization of Chicken FGF6 Gene and Association of Its Polymorphisms with Economic Traits [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1536-1550.
[4]	LUO Xiaoqin, GUO Zhiting, WANG He, ZHANG Jingyan, ZHANG Kang, WANG Lei, MA Yonghua, LI Jianxi. The Therapeutic Effect of Changshan Oral Liquid on Chicken Coccidiosis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1747-1755.
[5]	SU Chuanchen, JIAO Jingya, WANG Yongshuai, LUO Pengna, CHANG Xinghai, HUANG Yanqun. Effect of Introducing 25% Rhode Island Red Chicken Blood on Related Indexes of Henan Fighting Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1007-1018.
[6]	ZHENG Lin, WEI Bingdong, HUA Feng, CHEN Long, DING Yuan. Therapeutic Effect of Lytic Phage on Salmonella enteritidis Infection in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1314-1327.
[7]	REN Yuwei, CHEN Xing, LIN Yanning, HUANG Xiaoxian, HONG Lingling, WANG Feng, SUN Ruiping, ZHANG Yan, LIU Hailong, ZHENG Xinli, CHAO Zhe. Investigating the Influencing Factors of Egg Laying Performance in Wenchang Chickens Based on Whole Genome Resequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 502-514.
[8]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[9]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[10]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[11]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[12]	CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.
[13]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[14]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[15]	LIU Qing, WU Shaopeng, SHI Bin, SHAO Hongxia, QIAN Kun, YE Jianqiang, QIN Aijian. Expression of Chicken CTLA-4 Protein in Insect Cells and the Preparation of Its Monoclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2596-2604.