小麦饲料中添加木聚糖酶对高原型藏羊胃肠道发育的影响

doi:10.11843/j.issn.0366-6964.2023.01.021

Abstract

Abstract: The purpose of this experiment was to investigate the effect of xylanase (Xyl) added to wheat flour on the development of gastrointestinal tract of Tibetan lamb. Sixty plateau Tibetan lambs aged 2-3 months with good homogeneity were randomly divided into two groups with 30 lambs in each group and 5 replicates each. The control group was fed without xylanase, and the test group was fed the diet supplemented with 0.2% xylanase. The experimental period was divided into 10 days of pre-feeding period and 90 days of positive trial period. At the end of the feeding experiment, six lambs in each group were randomly selected for slaughter, the digestive tract tissues were collected and fixed in paraformaldehyde. And blood, the contents of rumen and jejunum were collected at the same time. The results showed that: 1) Compared with the control group, the thickness of rumen cuticle and granular layer, the length and width of omasum nipple, the thickness of omasum cuticle and granular layer, the thickness of abomasal mucosa, and duodenal muscle layer, the height of jejunal villi and the thickness of jejunum mucosa, the thickness of ileal muscle layer in the experimental group were significantly increased (P<0.05); 2) The contents of rumen and serum LPS in the control group were significantly higher than those in the experimental group (P<0.05); 3) Compared with the control group, the activities of rumen chymotrypsin, trypsin and lipase, the activities of jejunal chymotrypsin and α-Amylase increased significantly (P<0.05); 4) The total antioxidant capacity and the activities of glutathione peroxidase and catalase in rumen, the total antioxidant capacity of jejunum, the activities of glutathione peroxidase, superoxide dismutase and catalase of jejunum in the experimental group were significantly higher than those in the control group (P<0.05); 5) There was no significant difference in rumen and jejunum pH between the two groups (P>0.05). In conclusion, the addition of 0.2% xylanase to wheat feed can effectively reduce the concentration of LPS in rumen and serum of plateau Tibetan sheep, promote the morphological development of gastrointestinal tract, enhance digestive enzyme activity, and improve antioxidant capacity.

Key words: sheep, xylanase, gastrointestinal tract, digestive enzyme, antioxidant index, lipopolysaccharide, pH, wheat

CLC Number:

S826.5

ZHOU Li, GAO Zhanhong, ZHANG Fengshuo, GUI Linsheng, HOU Shengzhen, HAN Lijuan, SUN Shengnan, YUAN Zhenzhen. Effects of Xylanase Supplementation in Wheat Diet on Gastrointestinal Development of Plateau Tibetan Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 227-238.

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Effects of Xylanase Supplementation in Wheat Diet on Gastrointestinal Development of Plateau Tibetan Sheep

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