基于血浆非靶代谢组学探究急性笼养应激对蛋鸭代谢的影响

doi:10.11843/j.issn.0366-6964.2022.12.013

Abstract

Abstract: Investigating caging stress in laying ducks has become extremely important since the environmental policies have changed and the duck industry has grown. This study aims to explore the plasma metabolite profiles of laying duck raised in floor-water rearing system (FWR) and cage-rearing system (CR) using nontargeted metabolomics. The metatarsal vein blood was taken from ten laying ducks from each experimental group for HPLC-HRMS. Differential metabolites were identified with Fold-change ratio≥2 or ≤1/2, VIP>1 and q value<0.05. A total of 30 differential metabolites were obtained based on the screening conditions, of which 14 were up-regulated (P<0.05) and 16 were down-regulated (P<0.05). Differential metabolites included 16 glycerophospholipids, 4 fatty acyls, 2 carboxylic acids and derivatives, as well as phenylpropionic acid, phenols, organic sulfuric acids and derivatives, sterol lipids, purine nucleosides, organooxygen compounds, steroids and steroid derivatives, steroids and steroid derivatives. Primary bile acid biosynthesis and glycerophospholipid metabolism (P<0.01), as well as the secondary bile acid biosynthesis and taurine and hypotaurine metabolism (P<0.05) were found to be enriched by the pathway enrichment analysis. The results showed substantial variations in plasma bile acids, taurine, and glycerophospholipids between FWR and CR. The insights gained from this study may be useful for the development and promotion of waterfowl husbandry by clarifying the mechanism of caging stress in laying duck industry.

Key words: cage-rearing laying ducks, floor-water combination rearing system, nontargeted metabolomics, cage-rearing stress, plasma

CLC Number:

S834.83

LIU Shuibing, FANG Wenjie, LI Yankai, ZHANG Wentao, LIU Sanfeng, CHEN Biao. Effect of Acute Cage Stress on the Metabolism of Laying Ducks Studied by Plasma Nontargeted Metabolomics[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4271-4282.

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Effect of Acute Cage Stress on the Metabolism of Laying Ducks Studied by Plasma Nontargeted Metabolomics

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