H2S暴露对保育猪氧化还原状态及硫化氢代谢的影响

doi:10.11843/j.issn.0366-6964.2022.09.021

Abstract

Abstract: This study aimed to investigate the effects of hydrogen sulfide (H₂S) exposure on redox status and endogenous H₂S metabolism in nursery pigs. Twelve 35-day-old healthy Large White pigs with similar body weight ((11.61±1.51) kg) were randomly divided into two artificial environmental cabins with 6 pigs in each group, half male and half female per group. Pigs in the experimental group were exposed to 30 mg·m^-3 H₂S for 28 days, while pigs in the control group were kept without H₂S. Serum and liver samples were collected from each pig at the end of the experiment to detect the indexes related to redox and H₂S metabolism. The results showed that compared with the control group:1) In serum, the content of ROS increased extremely significantly (P<0.01) and MDA increased significantly (P<0.05); The activity of the antioxidant enzyme T-SOD decreased significantly (P<0.05), the activity of CAT and GPX decreased extremely significantly (P<0.01); The contents of non-enzymatic antioxidant GSH and GSSG did not change significantly (P>0.05). 2) In liver, the activities of T-SOD and GPX increased extremely significantly (P<0.01); the ability of scavenging ·OH improved significantly (P<0.05); The contents of ROS, H₂O₂, PC, MDA, GSH and GSSG showed no significant difference (P>0.05). 3) In liver, the mRNA expression of Keap1 was significantly down-regulated (P<0.05) and Nrf2 was significantly up-regulated (P<0.05); The mRNA expression of the antioxidant-related gene (SOD2, GPX1, GPX2, GPX4 and GSR) were significantly up-regulated (P<0.05). 4) The concentration of H₂S in serum and liver decreased significantly (P<0.05); The mRNA expressions of endogenous H₂S synthase CSE and CBS in the liver were significantly down-regulated (P<0.05) while that of 3-MST was not significantly different (P>0.05), and the mRNA expressions of H₂S catabolic enzyme SQR and SUOX showed no significant change either (P>0.05). The results indicated that 30 mg·m^-3 H₂S exposure caused damage to the serum antioxidant system of nursery pigs, but the activation of the Nrf2/Keap1 signaling pathway in liver protected the liver from oxidative damage. In addition, H₂S exposure inhibited the anabolism of endogenous H₂S in nursery pigs.

Key words: hydrogen sulfide (H₂S), nursery pigs, redox status, metabolism

CLC Number:

CHEN Lei, LIU Zhen, XIE Yanjiao, MIAO Qixiang, LUO Chengzeng, ZHANG Hongfu, TANG Xiangfang. Effects of H₂S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3052-3062.

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Effects of H₂S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs

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Effects of H2S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs

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Effects of H₂S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs