关中奶山羊源D型产气荚膜梭菌全基因组序列测定及其分子特征分析

doi:10.11843/j.issn.0366-6964.2022.11.022

Abstract

Abstract: In our study, five Clostridium perfringens isolates were obtained from diarrhea Guanzhong diary goats and named as 21-D-1 to 21-D-5. Toxin genes detection indicated the isolates all belonged to C. perfringens type D positive for etx and cpa, while cpe associated with foodborne diseases was detected in strain 21-D-5. After whole genome sequencing, we found the genome size, GC content and genes number were stable among the C. perfringens type D genomes. SNPs analysis showed there were few SNPs (<25) between 21-D-1 and 21-D-2, and 21-D-3 and 21-D-4, which revealed they might belonged to two C. perfringens strains, respectively. Fifteen toxin gens were detected among the C. perfringens type D isolates, sequence and genetic environment analysis of etx indicated sequence of etx were highly conserved and the genetic environments were similar among the genomes. In addition, cpe was detected downstream etx in the genome of C. perfringens strain 21-D-5. Besides, oxazolidinones-resistant gene optrA and the other eight antimicrobial resistant genes were also detected among the isolates, and erm(A), optrA and fexA were observed to have the the possibility of co-spread. This is the first study in China, which whole genome sequence and analyze the genomes of C. perfringens type D strains. The results of our study may provide reference for prevention and treatment of C. perfringens diseases, and further researches on C. perfringens genomes.

Key words: Guanzhong dairy goats, Clostridium perfringens type D, whole genome sequencing

CLC Number:

S852.616.3

WU Ke, FENG Hang, WANG Juan, YANG Zengqi. Whole Genome Sequencing and Molecular Characterization Analysis of Clostridium perfringens Type D Strains from Guanzhong Dairy Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3967-3974.

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Whole Genome Sequencing and Molecular Characterization Analysis of Clostridium perfringens Type D Strains from Guanzhong Dairy Goats

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