分泌IgA和IgG的浆细胞在家兔鼻腔中的分布特征

doi:10.11843/j.issn.0366-6964.2022.08.032

Abstract

Abstract: Plasma cells secreting IgA and IgG are two important effector cells in mucosal immune system. This study aimed to explore the distribution characteristics of plasma cells secreting IgA and IgG in the nasal cavities of rabbits. Twenty healthy adult rabbits were selected as the research objects. According to the characteristics of the hard palate folds and teeth, the nasal cavities of rabbits were divided into five segments,including Ⅰ Ⅱ Ⅲ Ⅳ and Ⅴ segments. Histology, immunohistochemistry, image analysis and statistical methods were used to carefully investigate the distribution characteristics of plasma cells secreting IgA and IgG in nasal cavities of rabbits. Immunohistochemical results showed that plasma cells secreting IgA and IgG were located in every segment of the nasal cavities of rabbits, diffusedly distributed in the lamina propria of nasal mucosa. The morphology was round or oval, and the nucleus was usually located at the side of the cells. Abundant cytoplasm was positive. Statistical results showed that the distribution density of plasma cells secreting IgA and IgG in different segments of nasal cavities from high to low was Ⅳ segment, Ⅴ segment, Ⅲ segment, Ⅱ segment and Ⅰ segment of nasal cavities. The distribution density of plasma cells secreting IgA in Ⅲ, Ⅳ and Ⅴ segments of the nasal cavities was significantly higher than that in Ⅰ and Ⅱ segments (P<0.05). The distribution density of plasma cells secreting IgA in maxilloturbinate, ethmoid turbinate and nasal septum was significantly higher than that in the superior tuibinate (P<0.05). Compared with plasma cells secreting IgA, plasma cells secreting IgG have similar distribution trend except that distribution density of plasma cells secreting IgG in the Ⅳ segment of the nasal cavities was significantly higher than that in Ⅲ and Ⅴ segments(P<0.05). The results confirmed that plasma cells secreting IgA and IgG were distributed diffusedly in the nasal mucosa propria of each segment of the rabbit nasal cavity, which would be conducive to SIgA and IgG molecule formation complete protective barrier in the whole nasal mucosa. Plasma cells secreting IgA and IgG were mainly distributed in Ⅲ, Ⅳ, Ⅴ segments of the nasal cavities, maxilloturbinate, ethmoid turbinate and nasal septum, indicating that these areas are important effective sites of intranasal immunization in rabbits. This study will provide an important theoretical basis for the prevention of rabbit's respiratory diseases and further exploration of the mechanism of nasal immune response in rabbits.

Key words: rabbit, nasal cavity, plasma cells secreting IgA, plasma cells secreting IgG, distribution

CLC Number:

S852.162

FANG Yingdong, CHENG Cuicui, LU Jia, CHENG Yujiao, ZHANG Rui, LI Peixuan, WANG Wenhui. Distribution Characteristics of Plasma Cells Secreting IgA and IgG in Rabbit Nasal Cavity[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2751-2762.

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Distribution Characteristics of Plasma Cells Secreting IgA and IgG in Rabbit Nasal Cavity

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