分泌IgA和IgG的浆细胞在家兔鼻腔中的分布特征

doi:10.11843/j.issn.0366-6964.2022.08.032

Abstract

Abstract: Plasma cells secreting IgA and IgG are two important effector cells in mucosal immune system. This study aimed to explore the distribution characteristics of plasma cells secreting IgA and IgG in the nasal cavities of rabbits. Twenty healthy adult rabbits were selected as the research objects. According to the characteristics of the hard palate folds and teeth, the nasal cavities of rabbits were divided into five segments,including Ⅰ Ⅱ Ⅲ Ⅳ and Ⅴ segments. Histology, immunohistochemistry, image analysis and statistical methods were used to carefully investigate the distribution characteristics of plasma cells secreting IgA and IgG in nasal cavities of rabbits. Immunohistochemical results showed that plasma cells secreting IgA and IgG were located in every segment of the nasal cavities of rabbits, diffusedly distributed in the lamina propria of nasal mucosa. The morphology was round or oval, and the nucleus was usually located at the side of the cells. Abundant cytoplasm was positive. Statistical results showed that the distribution density of plasma cells secreting IgA and IgG in different segments of nasal cavities from high to low was Ⅳ segment, Ⅴ segment, Ⅲ segment, Ⅱ segment and Ⅰ segment of nasal cavities. The distribution density of plasma cells secreting IgA in Ⅲ, Ⅳ and Ⅴ segments of the nasal cavities was significantly higher than that in Ⅰ and Ⅱ segments (P<0.05). The distribution density of plasma cells secreting IgA in maxilloturbinate, ethmoid turbinate and nasal septum was significantly higher than that in the superior tuibinate (P<0.05). Compared with plasma cells secreting IgA, plasma cells secreting IgG have similar distribution trend except that distribution density of plasma cells secreting IgG in the Ⅳ segment of the nasal cavities was significantly higher than that in Ⅲ and Ⅴ segments(P<0.05). The results confirmed that plasma cells secreting IgA and IgG were distributed diffusedly in the nasal mucosa propria of each segment of the rabbit nasal cavity, which would be conducive to SIgA and IgG molecule formation complete protective barrier in the whole nasal mucosa. Plasma cells secreting IgA and IgG were mainly distributed in Ⅲ, Ⅳ, Ⅴ segments of the nasal cavities, maxilloturbinate, ethmoid turbinate and nasal septum, indicating that these areas are important effective sites of intranasal immunization in rabbits. This study will provide an important theoretical basis for the prevention of rabbit's respiratory diseases and further exploration of the mechanism of nasal immune response in rabbits.

Key words: rabbit, nasal cavity, plasma cells secreting IgA, plasma cells secreting IgG, distribution

CLC Number:

S852.162

FANG Yingdong, CHENG Cuicui, LU Jia, CHENG Yujiao, ZHANG Rui, LI Peixuan, WANG Wenhui. Distribution Characteristics of Plasma Cells Secreting IgA and IgG in Rabbit Nasal Cavity[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2751-2762.

References 33

[1]	WALKER A, SURDA P, ROSSITER M, et al.Nasal function and dysfunction in exercise[J].J Laryngol Otol, 2016, 130(5):431-434.
[2]	MAROOF A, YORGENSEN Y M, LI Y F, et al.Intranasal vaccination promotes detrimental Th17-mediated immunity against influenza infection[J].PLoS Pathog, 2014, 10(1):e1003875.
[3]	YANG J J, DAI L, YU Q H, et al.Histological and anatomical structure of the nasal cavity of Bama minipigs[J].PLoS One, 2017, 12(3):e0173902.
[4]	高小玲, 蒋新国.鼻腔免疫研究进展[J].中国药学杂志, 2004, 39(7):486-490.GAO X L, JIANG X G.Progress of studies on intranasal immunization[J].Chinese Pharmaceutical Journal, 2004, 39(7):486-490.(in Chinese)
[5]	DEBIN A, KRAVTZOFF R, SANTIAGO J V, et al.Intranasal immunization with recombinant antigens associated with new cationic particles induces strong mucosal as well as systemic antibody and CTL responses[J].Vaccine, 2002, 20(21-22):2752-2763.
[6]	STRUGNELL R A, WIJBURG O L C.The role of secretory antibodies in infection immunity[J].Nat Rev Microbiol, 2010, 8(9):656-667.
[7]	HAPFELMEIER S, LAWSON M A E, SLACK E, et al.Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses[J].Science, 2010, 328(5986):1705-1709.
[8]	JUNGHANS R P, ANDERSON C L.The protection receptor for IgG catabolism is the β2-microglobulin-containing neonatal intestinal transport receptor[J].Proc Natl Acad Sci U S A, 1996, 93(11):5512-5516.
[9]	周光炎.免疫学原理[M].4版.北京:科学出版社, 2018.ZHOU G Y.Principles of immunology[M].4th ed.Beijing:Science Press, 2018.(in Chinese)
[10]	王慧.家兔的饲养管理[J].中国养兔, 2020(2):37-38.WANG H.Breeding and management of rabbits[J].Chinese Journal of Rabbit Farming, 2020(2):37-38.(in Chinese)
[11]	谷子林.家兔呼吸道疾病病因及其防控技术[J].中国养兔, 2020(2):33-36.GU Z L.Etiology and prevention technology of respiratory diseases in rabbits[J].Chinese Journal of Rabbit Farming, 2020(2):33-36.(in Chinese)
[12]	袁丽英, 王娟, 邓胜朝, 等.鼻腔免疫疫苗的前景和未来面临的挑战[J].中国动物传染病学报, 2021, 29(2):114-118.YUAN L Y, WANG J, DENG S C, et al.Current prospects and future challenges for nasal vaccine delivery[J].Chinese Journal of Animal Infectious Diseases, 2021, 29(2):114-118.(in Chinese)
[13]	ZHANG W D, ZHANG X F, CHENG C C, et al.Impact of aging on distribution of IgA+ and IgG+cells in aggregated lymphoid nodules area in abomasum of Bactrian camels (Camelus bactrianus)[J].Exp Gerontol, 2017, 100:36-44.
[14]	扎西英派.阿拉善双峰驼大肠MALT的诱导部位PPs的研究[D].兰州:甘肃农业大学, 2014.ZHA X Y P.The study of mucosal inductive site (PPs) in mucosa-associated lymphoid tissue in the large intestine of Alxa Bactrian camel[D].Lanzhou:Gansu Agricultural University, 2014.(in Chinese)
[15]	ZHANG W D, WANG W H, JIA S.The distribution of SIgA and IgG antibody-secreting cells in the small intestine of Bactrian camels (Camelus bactrianus) of different ages[J].PLoS One, 2016, 11(6):e0156635.
[16]	曹婷婷.IgA、IgG和IgM分泌细胞在家兔胃肠道的分布规律及其与年龄的关系[D].兰州:甘肃农业大学, 2016.CAO T T.The relationship between the distribution of IgA, IgG and IgM secreting-cells in the gastrointestinal tract and ages in rabbits[D].Lanzhou:Gansu Agricultural University, 2016.(in Chinese)
[17]	JIA S, ZHANG W D, TAN X F, et al.The distribution of SIgA and IgG antibody-secreting cells in the palatine tonsils of Bactrian camels (Camelus bactrianus) of different ages[J].Histol Histopathol, 2017, 32(4-5):511-521.
[18]	杨树宝, 徐晶, 张桂山, 等.不同日龄鸡呼吸道黏膜中IgA分泌细胞的分布规律[J].华南农业大学学报, 2021, 42(5):27-32.YANG S B, XU J, ZHANG G S, et al.Distribution of IgA secreting cells in respiratory mucosa of chicken at different day-age[J].Journal of South China Agricultural University, 2021, 42(5):27-32.(in Chinese)
[19]	李乙江, 杨晶晶, 牛自兵, 等.黄牛呼吸道IgA分泌细胞和淋巴组织分布的研究[J].南京农业大学学报, 2017, 40(6):1100-1104.LI Y J, YANG J J, NIU Z B, et al.Distribution of IgA secreting cells and lymphoid tissues in cattle respiratory tract[J].Journal of Nanjing Agricultural University, 2017, 40(6):1100-1104.(in Chinese)
[20]	URAIH L C, MARONPOT R R.Normal histology of the nasal cavity and application of special techniques[J].Environ Health Perspect, 1990, 85:187-208.
[21]	ASANO M, KOMIYAMA K.Polymeric immunoglobulin receptor[J].J Oral Sci, 2011, 53(2):147-156.
[22]	JOHANSEN F E, KAETZEL C S.Regulation of the polymeric immunoglobulin receptor and IgA transport:New advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity[J].Mucosal Immunol, 2011, 4(6):598-602.
[23]	何晚红.成年双峰驼支气管树及黏膜免疫屏障特征的研究[D].兰州:甘肃农业大学, 2019.HE W H.Studies on the bronchial tree and the characteristics of mucosal immune barrier in adult Bactrian camels[D].Lanzhou:Gansu Agricultural University, 2019.(in Chinese)
[24]	张学锋, 李淑娴, 李航, 等.IgA和IgG分泌细胞在双峰驼CALT中的分布规律[J].中国兽医学报, 2016, 36(2):353-356.ZHANG X F, LI S X, LI H, et al.IgA and IgG secreting cells in CALT of Bactrian camel[J].Chinese Journal of Veterinary Science, 2016, 36(2):353-356.(in Chinese)
[25]	车传燕, 杨倩.断奶仔猪胃肠道中IgA和IgG分泌细胞的分布[J].畜牧兽医学报, 2010, 41(5):615-620.CHE C Y, YANG Q.The distribution of IgA and IgG secreting cells in the gastrointestinal tract of the weaning piglets[J].Acta Veterinaria et Zootechnica Sinica, 2010, 41(5):615-620.(in Chinese)
[26]	曹星星, 胡利, 何彦明, 等.SIgA在肠道免疫和自身免疫性疾病中的研究进展[J].医学综述, 2021, 27(13):2529-2533.CAO X X, HU L, HE Y M, et al.Research advances of secretory immunoglobulin A in intestinal immunity and autoimmune diseases[J].Medical Recapitulate, 2021, 27(13):2529-2533.(in Chinese)
[27]	李鹏成, 刘志学, 高君恺, 等.猪呼吸道IgA和IgG分泌细胞的分布[J].畜牧兽医学报, 2010, 41(7):873-877.LI P C, LIU Z X, GAO J K, et al.Distribution of IgA and IgG secreting cell in porcine respiratory tract[J].Acta Veterinaria et Zootechnica Sinica, 2010, 41(7):873-877.(in Chinese)
[28]	曾梅, 徐树培, 曾洪良, 等.家兔几种呼吸道疾病的临床鉴别诊断和防治措施[J].畜禽业, 2020, 31(11):86-87.ZENG M, XU S P, ZENG H L, et al.Clinical differential diagnosis and preventive measures of several respiratory diseases in rabbits[J].Livestock and Poultry Industry, 2020, 31(11):86-87.(in Chinese)
[29]	程翠翠, 李建飞, 张旺东, 等.家兔鼻腔的形态学特点及NALT分布规律的研究[J].动物医学进展, 2018, 39(11):79-83.CHENG C C, LI J F, ZHANG W D, et al.Study on morphology characteristics and distribution of NALT in rabbit nasal cavity[J].Progress in Veterinary Medicine, 2018, 39(11):79-83.(in Chinese)
[30]	BALL J M, HARDY M E, ATMAR R L, et al.Oral immunization with recombinant Norwalk virus-like particles induces a systemic and mucosal immune response in mice[J].J Virol, 1998, 72(2):1345-1353.
[31]	CORTHÉSY B.Multi-faceted functions of secretory IgA at mucosal surfaces[J].Front Immunol, 2013, 4:185.
[32]	陈耀星, 崔燕.动物解剖学与组织胚胎学(全彩版)[M].北京:中国农业出版社, 2019.CHEN Y X, CUI Y.Animal anatomy and histoembryology[M].Beijing:China Agriculture Press, 2019.(in Chinese)
[33]	LATVALA S, JACOBSEN B, OTTENEDER M B, et al.Distribution of FcRn across species and tissues[J].J Histochem Cytochem, 2017, 65(6):321-333.

Distribution Characteristics of Plasma Cells Secreting IgA and IgG in Rabbit Nasal Cavity

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