SMAD7对山羊卵泡颗粒细胞增殖、凋亡的影响

doi:10.11843/j.issn.0366-6964.2022.08.013

Abstract

Abstract: The purpose of this study was to explore the effects of SMAD7 on the proliferation and apoptosis of follicular granulosa cells in goat. In this study, follicular granulosa cells were collected from 3-to 4-month-old Dazu black goats. The overexpression, siRNA interference, ELISA, qRT-PCR, Western blot and flow cytometry were used to investigate the effects of SMAD7 on proliferation, apoptosis and steroid secretion of granulosa cells. The results showed that overexpression of SMAD7 significantly reduced the proliferation activity and promoted the apoptosis of granulosa cells, inhibited the expression of PCNA (P<0.05) and decreased the ratio of BCL2/BAX(P<0.01). Oppositely, SMAD7 interference significantly promoted the proliferation activity of granulosa cells, significantly up-regulated the expression of PCNA and the ratio of BCL2/BAX (P<0.05).SMAD7 overexpression significantly increased progesterone secretion of granulated cells, and reduced estradiol expression levels (P<0.01). Meanwhile,SMAD7 interference significantly down-regulated the secretion of progesterone and up-regulated the secretion of estradiol (P<0.01). Further studies showed that SMAD7 overexpression significantly inhibited SMAD2,SMAD3 mRNA and protein expression (P<0.05), but did not affect SMAD4 expression. SMAD7 interference significantly promoted the mRNA and protein levels of SMAD2,SMAD3 (P<0.05). The results suggested that SMAD7 inhibits the proliferation and estradiol secretion, promotes apoptosis and progesterone synthesis, and inhibits the expression of SMAD2,SMAD3 in follicular granulosa cells, thereby regulating the development and atresia of follicles.

Key words: SMAD7, goat, granulosa cell, proliferation, apoptosis

CLC Number:

S827.3

LI Bijun, HUANG Siyi, WANG Yukun, WANG Lei, HE Lina, TANG Xue, XU Dejun, ZHAO Zhongquan. Effects of SMAD7 on Proliferation and Apoptosis of Goat Follicular Granulosa Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2548-2557.

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Effects of SMAD7 on Proliferation and Apoptosis of Goat Follicular Granulosa Cells

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