骆驼乳清蛋白对热应激所致大鼠肝氧化损伤的保护作用

doi:10.11843/j.issn.0366-6964.2021.09.028

Abstract

Abstract: This study aimed to investigate the protective effects of camel whey protein (CWP) on liver injury, oxidative stress, and liver function of rats under heat-stressed (HS). Thirty six 6-week-old SD rats were selected and randomly divided into 6 groups after 2 weeks of adaptive feeding:the control group fed a basal diet, the CWP control group supplemented with 400 mg·kg^-1of CWP, the HS group received 2 h HS treatment per day for 8 d in addition to the basal diet, and the CWP intervention group received 100, 200, and 400 mg·kg^-1of CWP before each HS treatment. Liver histological changes were observed by HE staining, and liver function biomarkers, oxidative stress markers, and antioxidant enzyme activities were detected. The results showed as follows:CWP intervention reduced HS serum aspartate transaminase (AST) and alanine transaminase (ALT) activities, and 400 mg·kg^-1CWP had the best effect (P<0.01). And 400 mg·kg^-1CWP effectively reduced HS-induced histopathological changes in the rat liver. CWP suppressed hepatic reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and enhanced superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as glutathione (GSH) levels, with 400 mg·kg^-1CWP having the best effect (P<0.01). These results suggested that CWP intervention could alleviate HS-induced liver injury in rats by suppressing hepatic oxidative stress and enhancing antioxidant system defenses in a dose-dependent manner.

Key words: camel whey protein, rat, liver injury, oxidative stress, heat stress

CLC Number:

Q593.1

WUEN Jiya, MA Xueni, DU Donghua, HASI Surong. Protective Effects of Camel Whey Protein against Oxidative Damage in Rat Liver Induced by Heat Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2642-2649.

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Protective Effects of Camel Whey Protein against Oxidative Damage in Rat Liver Induced by Heat Stress

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