CTGF和FGF-2在不同年龄牦牛肺内的分布与表达研究

doi:10.11843/j.issn.0366-6964.2021.07.024

Abstract

Abstract: The exact distribution and expression of CTGF and FGF-2 in the lungs of newborn, young, adult and old yaks were studied by immunohistochemistry (IHC), Western blot and real-time fluorescence quantitative (qRT-PCR). The results of IHC showed that CTGF was mainly distributed in terminal bronchiole epithelium Clara cells and smooth muscle cells, pulmonary artery endothelial cells and medium membrane smooth muscle cells; The expression levels were the highest in the newborn and elderly groups; FGF-2 was mainly distributed in the smooth muscle of the terminal bronchiole and the smooth muscle of the pulmonary artery, and maintained a high expression level in all age groups. The results of WB showed that the expression of CTGF and FGF-2 could be detected in the lungs of yaks in different age groups, the expression of CTGF in newborn group and elderly group was significantly higher than that in young group and adult group (P<0.05), and the expression of FGF-2 in the newborn group and young group was significantly higher than that in adult group and old group (P<0.05). The results of qRT-PCR showed that the transcriptional level of CTGF was the highest in the newborn group, and there were significant differences among different age groups, while the transcriptional level of FGF-2 in the elderly group was the highest, followed by the adult group, and there were significant differences compared with other groups (P<0.05). CTGF and FGF-2 are related to the formation of adaptive structure and adaptive process of yak lung, and play a significant role in newborn and old age.

Key words: yak, lung, connective tissue growth factor (CTGF), basic fibroblast growth factor 2 (FGF-2)

CLC Number:

S852.1

LI Yuejiao, CUI Yan, ZHANG Qian, HE Junfeng. Study on the Distribution and Expression of CTGF and FGF-2 in the Lungs of Yaks at Different Ages[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2025-2033.

References 26

[1]	陈秋生, 冯霞, 姜生成. 牦牛肺脏高原适应性的结构研究[J]. 中国农业科学, 2006, 39(10):2107-2113.CHEN Q S, FENG X, JIANG S C. Structural study on plateau adaptability of yak lung[J]. Scientia Agricultura Sinica, 2006, 39(10):2107-2113. (in Chinese)
[2]	何俊峰, 余四九, 崔燕. 不同年龄高原牦牛肺脏的组织结构特征[J]. 畜牧兽医学报, 2009, 40(5):748-755.HE J F, YU S J, CUI Y. Characteristics of lung structure in different age plateau yak[J]. Acta Veterinaria et Zootechnica Sinica, 2009, 40(5):748-755. (in Chinese)
[3]	BRADHAM D M, IGARASHI A, POTTER R L, et al. Connective tissue growth factor:a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10[J]. J Cell Biol, 1991, 114(6):1285-1294.
[4]	YANG Z Z, SUN Z R, LIU H M, et al. Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat-induced pulmonary fibrosis[J]. Mol Med Rep, 2015, 12(1):1091-1097.
[5]	BURGOS C M, NORD M, ROOS A, et al. Connective tissue growth factor expression pattern in lung development[J]. Exp Lung Res, 2010, 36(8):441-450.
[6]	梁潇. 低压低氧时细胞因子的变化特点及其与心肌纤维化的关系[D]. 重庆:重庆医科大学, 2016.LIANG X. Changes of some cytokines and its correlation with myocardial fibrosis under hypobaric hypoxia[D]. Chongqing:Chongqing Medical University, 2016. (in Chinese)
[7]	TODA N, MUKOYAMA M, YANAGITA M, et al. CTGF in kidney fibrosis and glomerulonephritis[J]. Inflamm Regen, 2018, 38:14.
[8]	崔茂香, 陈晓玲, 霍春玲, 等. 结缔组织生长因子在肺纤维化初期肺动脉中的表达[J]. 生理学报, 2008, 60(4):535-540.CUI M X, CHEN X L, HUO C L, et al. Expression of connective tissue growth factor in pulmonary artery at the early-stage of pulmonary fibrosis[J]. Acta Physiologica Sinica, 2008, 60(4):535-540. (in Chinese)
[9]	GOTO A, YAMAZAKI N, NOGAWA H. Characterization of FGF family growth factors concerning branching morphogenesis of mouse lung epithelium[J]. Zoolog, 2014, 31(5):267-273.
[10]	COFFEY E, NEWMAN D R, SANNES P L. Expression of fibroblast growth factor 9 in normal human lung and idiopathic pulmonary fibrosis[J]. J Histochem Cytochem, 2013, 61(9):671-679.
[11]	GUZY R D, STOILOV I, ELTON T J, et al. Fibroblast growth factor 2 is required for epithelial recovery, but not for pulmonary fibrosis, in response to bleomycin[J]. Am J Respir Cell Mol Biol, 2015, 52(1):116-128.
[12]	EL AGHA E, SEEGER W, BELLUSCI S. Therapeutic and pathological roles of fibroblast growth factors in pulmonary diseases[J]. Dev Dyn, 2017, 246(4):235-244.
[13]	张蔷, 林勇. CTGF在低氧性肺血管重建中的作用[J]. 东南大学学报:医学版, 2005, 24(6):395-398.ZHANG Q, LIN Y. Expression of CTGF in pulmonary vessels of hypoxemic pulmonary hypertensive mouse[J]. Journal of Southeast University:Medical Science Edition, 2005, 24(6):395-398. (in Chinese)
[14]	蒋寒丹, 何俊峰, 崔燕. CTGF和FGF-2在成年牦牛肺脏的分布[J]. 中国兽医学报, 2017, 37(5):888-892.JIANG H D, HE J F, CUI Y. Distribution of CTGF and FGF-2 in adult yak lung[J]. Chinese Journal of Veterinary Science, 2017, 37(5):888-892. (in Chinese)
[15]	DURMOWICZ A G, HOFMEISTER S, KADYRALIEV T K, et al. Functional and structural adaptation of the yak pulmonary circulation to residence at high altitude[J]. J Appl Physiol, 1993, 74(5):2276-2285.
[16]	PENALOZA D, ARIAS-STELLA J. The heart and pulmonary circulation at high altitudes:healthy highlanders and chronic mountain sickness[J]. Circulation, 2007, 115(9):1132-1146.
[17]	ALAPATI D, RONG M, CHEN S Y, et al. Connective tissue growth factor antibody therapy attenuates hyperoxia-induced lung injury in neonatal rats[J]. Am J Respir Cell Mol Biol, 2011, 45(6):1169-1177.
[18]	YANG Z Z, SUN Z R, LIU H M, et al. Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat-induced pulmonary fibrosis[J]. Mol Med Rep, 2015, 12(1):1091-1097.
[19]	华锋, 崔恩海, 林勇. 缺氧对肺成纤维细胞表达结缔组织生长因子的影响[J]. 中国呼吸与危重监护杂志, 2006, 5(4):301-303.HUA F, CUI E H, LIN Y. Effects of hypoxia on the expression of connective tissue growth factor in lung fibroblast[J]. Chinese Journal of Respiratory and Critical Care Medicine, 2006, 5(4):301-303. (in Chinese)
[20]	高艳, 谢敏, 何正平, 等. 缺氧不同时间对人胚肺成纤维细胞RHO/RHO激酶信号通路及CTGF表达的影响[J]. 四川大学学报:医学版, 2010, 41(6):946-950.GAO Y, XIE M, HE Z P, et al. The effect of hypoxia on the expression of RHO/RHO signaling pathway and CTGF of human embryonic fibroblasts[J]. Journal of Sichuan University:Medical Science Edition, 2010, 41(6):946-950. (in Chinese)
[21]	符红娜, 孙兆瑞, 葛鑫, 等. 结缔组织生长因子在百草枯致肺纤维化中的作用[J]. 中华急诊医学杂志, 2018, 27(11):1198-1204.FU H N, SUN Z R, GE X, et al. The role of connective tissue growth factor in pulmonary fibrosis induced by paraquat[J]. Chinese Journal of Emergency Medicine, 2018, 27(11):1198-1204. (in Chinese)
[22]	文文, 赖国祥, 柳德灵, 等. 反复细菌感染致大鼠气道重构中结缔组织生长因子的表达变化及意义[J]. 中国病理生理杂志, 2012, 28(3):546-549.WEN W, LAI G X, LIU D L, et al. Role of connective tissue growth factor in airway remodeled in rats induced by repeated bacterial infection[J]. Chinese Journal of Pathophysiology, 2012, 28(3):546-549. (in Chinese)
[23]	CAMERLIET P, JAIN R K. Molecular mechanisms and clinical applications of angiogenesis[J]. Nature, 2011, 473(7347):298-307.
[24]	CARMELIET P. Mechanisms of angiogenesis and arteriogenesis[J]. Nat Med, 2000, 6(4):389-395.
[25]	LITWIN M, RADWAŃSKA A, PAPROCKA M, et al. The role of FGF2 in migration and tubulogenesis of endothelial progenitor cells in relation to pro-angiogenic growth factor production[J]. Mol Cell Biochem, 2015, 410(1-2):131-142.
[26]	REN H, BIRCH N P, SURESH V. An optimised human cell culture model for alveolar epithelial transport[J]. PLoS ONE, 2016, 11(10):e0165225.

Study on the Distribution and Expression of CTGF and FGF-2 in the Lungs of Yaks at Different Ages

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