猪丹毒丝菌CbpB蛋白对小鼠的免疫效果分析

doi:10.11843/j.issn.0366-6964.2021.06.023

Abstract

Abstract: This study aimed to explore the immunogenicity and protective effects of CbpB protein of Erysipelothrix rhusiopathiae, and to provide new ideas and technical reserves for the further development of ER genetic engineering subunit vaccine. Mice were immunized with the expressed ER recombinant proteins CbpB, SpaA, CbpB+SpaA, ER inactivated whole cell (V-AEr21), commercial attenuated vaccine, and commercial inactivated vaccine, respectively. Indirect ELISA was used to detect the level of IgG antibodies. The challenge test was selected to determine the immune protection rate. Tissue bacteria count test was conducted to determine ER colonization. Mouse tissue organs (spleen, lung, liver, and kidney) were collected to prepare slices and observe pathological changes. Compared with the commercial attenuated vaccine with the strongest immunogenicity, the IgG antibody produced by CbpB and SpaA was only 1:6 400, but the bactericidal efficacy of serum was high. The immune challenge protection rate of mice was the same as that of commercial attenuated vaccine, which was 100%, and there was no bacterial colonization in spleen, lung, liver and kidney, and there was no significant difference between histopathological observation and blank control. There was no difference between CbpB and SpaA. CbpB recombinant protein has good immunogenicity and protective efficacy, can stimulate the body to produce humoral immunity and cellular immunity, and can be used as a candidate antigen for swine erysipelas genetic engineering subunit vaccine.

Key words: Erysipelothrix rhusiopathiae, CbpB protein, mouse, immunogenicity, immune protection

CLC Number:

S855.12

LIU Junwen, WU Qiongjuan, XING Gang, ZHAN Songhe, WEI Jianzhong, SUN Pei, LIU Xuelan, LI Yu. Immunogenicity Analysis and Protective Effects of CbpB Protein of Erysipelothrix rhusiopathiae in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1689-1699.

References 24

[1]	WANG Q N, CHANG B J, RILEY T V. Erysipelothrix rhusiopathiae[J]. Vet Microbiol, 2010, 140(3-4):405-417.
[2]	MCNEIL M, GERBER P F, THOMSON J, et al. Serotypes and Spa types of Erysipelothrix rhusiopathiae isolates from British pigs (1987 to 2015)[J]. Vet J, 2017, 225:13-15.
[3]	吴琼娟, 杨志鹏, 姚焱彬, 等. 猪红斑丹毒丝菌制苗用菌株的筛选[J]. 浙江农业学报, 2018, 30(9):1467-1475.WU Q J, YANG Z P, YAO Y B, et al. Screening of strains for inactivated vaccine of Erysipelothrix rhusiopathiae[J]. Acta Agriculturae Zhejiangensis, 2018, 30(9):1467-1475. (in Chinese)
[4]	王力波, 刘芳, 谢江, 等. 猪丹毒丝菌不同年代分离菌株流行病学特征及部分生物学特性比较研究[J]. 中国兽医学报, 2016, 36(3):423-430.WANG L B, LIU F, XIE J, et al. Comparative analysis on the epidemiological and some biological characteristics of Erysipelothrix rhusiopathiae isolates from different years[J]. Chinese Journal of Veterinary Science, 2016, 36(3):423-430. (in Chinese)
[5]	DING Y, ZHU D M, ZHANG J M, et al. Virulence determinants, antimicrobial susceptibility, and molecular profiles of Erysipelothrix rhusiopathiae strains isolated from China[J]. Emerg Microbes Infect, 2015, 4(11):e69.
[6]	魏文涛, 姚焱彬, 刘全喜, 等. 2012-2015年江淮地区猪丹毒杆菌分离株血清型、spaA基因遗传进化及PFGE基因型分析[J]. 微生物学通报, 2017, 44(3):664-672.WEI W T, YAO Y B, LIU Q X, et al. Analysis of serotypes, spaA gene phylogeny and PFGE genetic patterns of Erysipelothrix rhusiopathiae isolated between 2012 and 2015 in Jianghuai areas[J]. Microbiology China, 2017, 44(3):664-672. (in Chinese)
[7]	MORIMOTO M, KATO A, KOJIMA H, et al. Serovars and SpaA types of Erysipelothrix rhusiopathiae isolated from pigs in Japan from 2012 to 2019[J]. Curr Microbiol, 2021, 78(1):55-66.
[8]	ZHU W F, WANG Y, CAI C Z, et al. Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA[J]. FEMS Microbiol Lett, 2017, 364(5):fnx036.
[9]	ZHU W F, CAI C Z, WANG Y, et al. Characterization of roles of SpaA in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells[J]. Microb Pathog, 2017, 113:176-180.
[10]	ZHU W F, WU C, KANG C, et al. Evaluation of the protective efficacy of four newly identified surface proteins of Erysipelothrix rhusiopathiae[J]. Vaccine, 2018, 36(52):8079-8083.
[11]	ZHU W F, CAI C Z, LI J T, et al. Characterization of protective antigen CbpB as an adhesin and a plasminogen-binding protein of Erysipelothrix rhusiopathiae[J]. Res Vet Sci, 2019, 124:352-356.
[12]	BERGMANN S, HAMMERSCHMIDT S. Versatility of pneumococcal surface proteins[J]. Microbiology, 2001, 152(2):295-303.
[13]	SHI F, HARADA T, OGAWA Y, et al. Capsular polysaccharide of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, and its modification with phosphorylcholine[J]. Infect Immun, 2012, 80(11):3993-4003.
[14]	HADAD R, MARKS E, KALBINA I, et al. Protection against genital tract Chlamydia trachomatis infection following intranasal immunization with a novel recombinant MOMP VS2/4 antigen[J]. APMIS, 2016, 124(12):1078-1086.
[15]	杨振龙. 红斑丹毒丝菌的致病性研究及其表面蛋白的MALDI-TOF质谱分析[D]. 吉首:吉首大学, 2015.YANG Z L. Characterization of Erysipelothrix rhusiopathiae pathogenicity and identification of surface protein though MALDI-TOF-MS analysis[D]. Jishou:Jishou University, 2015. (in Chinese)
[16]	陈章, 刘晓露, 姚焱彬, 等. 猪丹毒丝菌灭活疫苗制备及其对小鼠免疫效力的评价[J]. 西北农业学报, 2019, 28(6):877-887.CHEN Z, LIU X L, YAO Y B, et al. Preparation of inactivated vaccine against Erysipelothrix rhusiopathiae and evaluation of immune efficacy in mice[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(6):877-887. (in Chinese)
[17]	辛伟. 副猪嗜血杆菌安徽株分型及tbpA基因的克隆表达和免疫保护作用的研究[D]. 合肥:安徽农业大学, 2011.XIN W. The research of typing Haemophilus parasuis and cloning expression of tbpA gene and analysis of the protection of the expressed protein[D]. Hefei:Anhui Agricultural University, 2011. (in Chinese)
[18]	李大鹏. 副猪嗜血杆菌病重组亚单位疫苗候选抗原蛋白的免疫保护效力评价[D]. 北京:中国农业科学院, 2016.LI D P. Evaluation of the immunoprotective efficacy of subunit vaccine candidates of Haemophilus parasuis serovar 5[D]. Beijing:Chinese Academy of Agricultural Sciences, 2016. (in Chinese)
[19]	LAMBKIN-WILLIAMS R, GELDER C, BROUGHTON R, et al. An intranasal proteosome-adjuvanted trivalent influenza vaccine is safe, immunogenic & efficacious in the human viral influenza challenge model. Serum IgG & Mucosal IgA are important correlates of protection against Illness associated with infection[J]. PLoS One, 2016, 11(12):e0163089.
[20]	姚焱彬, 陆萍, 杨志鹏, 等. 猪丹毒丝菌SpaA基因原核表达及表达蛋白质的免疫原性分析[J]. 畜牧兽医学报, 2017, 48(3):492-500.YAO Y B, LU P, YANG Z P, et al. Prokaryotic expression and immunogenicity analysis of SpaA gene in Erysipelothrix rhusiopathiae[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(3):492-500. (in Chinese)
[21]	罗满林. 动物传染病学[M]. 北京:中国林业出版社, 2013.LUO M L. Animal epidemiology[M]. Beijing:China Forestry Publishing House, 2013. (in Chinese)
[22]	赵德明. 兽医病理学[M]. 北京:中国农业大学出版社, 2005:133-135.ZHAO D M. Veterinary pathology[M]. Beijing:China Agricultural University Press, 2005:133-135. (in Chinese)
[23]	王平. 表达Ag85B-ESAT-6融合蛋白的重组耻垢分枝杆菌对结核分枝杆菌感染小鼠免疫治疗效果评价[D]. 西安:第四军医大学, 2014.WANG P. Immunotherapy efficacy of recombinant Mycobacterium smegmatis expressing Ag85B-ESAT-6 fusion protein against Mycobacterium tuberculosis infection in mice[D]. Xi'an:Fourth Military Medical University, 2014. (in Chinese)
[24]	杨汉春. 动物免疫学[M]. 2版. 北京:中国农业大学出版社, 2003.YANG H C. Animal immunology[M]. 2nd ed. Beijing:China Agricultural University Press, 2003. (in Chinese)

Immunogenicity Analysis and Protective Effects of CbpB Protein of Erysipelothrix rhusiopathiae in Mice

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