弓形虫和新孢子虫交叉反应抗原MIC7A对小鼠的免疫保护效力分析

doi:10.11843/j.issn.0366-6964.2022.07.026

Abstract

Abstract: Toxoplasma gondii and Neospora caninum are closely related Apicomplexa parasites. The identified cross-reacting antigens may account for the cross-protection between them. This study aimed to express and identify the cross-reacting antigen TgMIC17A to evaluate the cross immune-protective effect against T. gondii and N. caninum infection in mice. First, TgMIC17A was cloned and expressed in E. coli(DE3), and its cross reactivity and immunogenicity were analyzed by Western blot and ELISA, respectively. Then, mice were immunized with recombinant protein, and challenged with 1×10³ tachyzoites of T. gondii Pru or 1.5×10⁷ N. caninum Nc1 tachyzoites 10 days post the second immunization. The body weight change and survival rate of mice were recorded, and the parasite load in the brain was measured 30 days post-infection to assess the cross-protective efficacies of rTgMIC17A against T. gondii and N. caninum. The results indicated that recombinant TgMIC17A could be recognized by both positive sera of T. gondii and N. caninum. Compared with the unimmunized group, the immunized mice produced high level of specific IgG antibodies (P<0.01), and the amount of T. gondii or N. caninum load in brain significantly decreased as well (P<0.01). In summary, TgMIC17A was identified as a cross-reacting antigen between T. gondii and N. caninum, which can induce robust immune response to protect mice against T. gondii and N. caninum. Taken together, TgMIC17A is a potent cross-protective gene for use as recombinant vaccine against T. gondii or N. caninum infection.

Key words: MIC17A, Toxoplasma gondii, Neospora caninum, cross-reacting antigen, cross-immune protection

CLC Number:

S852.72

CHEN Huixian, CHEN Yajie, WANG Xianmei, WANG Lifang, LIU Qun, LIU Jing. Identification of the Cross-reacting Antigen MIC17A of Toxoplasma gondii and Neospora caninum and the Study of Its Cross-immune Protective Efficacy in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2300-2306.

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Identification of the Cross-reacting Antigen MIC17A of Toxoplasma gondii and Neospora caninum and the Study of Its Cross-immune Protective Efficacy in Mice

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