swnR基因在金龟子绿僵菌合成苦马豆素中的作用

doi:10.11843/j.issn.0366-6964.2021.05.030

Abstract

Abstract: Swainsonine (SW) is the principal toxic ingredient of locoweeds, and is produced by fungi. Yet the biosynthetic pathway and key catalytic enzyme genes are not quite clear. Studies have demonstrated that the biosythesis of SW requires for the presence of a variety of SWN cluster genes. In order to determine the role of the swnR gene encoding NADB Rossmann-fold reductase in this gene cluster, we used PEG-mediated homologous recombination (HR) to transform a wild-type strain. The concentration of SW was measured in the fermentation broth of M. anisopliae wild-type strain (WT), mutant-type strain (MT) and a complemented-type (CT) strain using a Q Exactive Mass Spectrometer. The content of swainsonine in the fermentation broth of the obtained WT, MT and CT were (82.91±15.92), (5.71±2.23), (56.42±10.82) μg·mg^-1, respectively. The results showed that the content of SW decreased in the fermentation broth of the MT strain, and returned to the original level in the CT strain. These results indicate that the swnR gene plays a crucial role in the SW biosynthesis pathway of M. anisopliae, this will lay a theoretical foundation for the follow-up study of the catalytic enzyme gene screening and biosynthetic pathway research of swainsonine synthesis of endophytic fungi in locoweed.

Key words: swainsonine, M. anisopliae, swnR gene, biosynthesis, gene knockout

CLC Number:

S476.12

SUN Lu, SONG Runjie, LU Hao, WANG Jinglong, MO Chonghui, ZHAO Baoyu. The Role of swnR Gene on the Biosynthetic Pathway of the Swainsonine in Metarhizium anisopliae[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1439-1446.

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The Role of swnR Gene on the Biosynthetic Pathway of the Swainsonine in Metarhizium anisopliae

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