金华猪回肠菌群结构和脂肪酸结合蛋白发育性变化与脂肪沉积的相关性研究

doi:10.11843/j.issn.0366-6964.2021.03.016

Abstract

Abstract: The objective of this study was to investigate the developmental changes of ileal microbiota and fatty acid binding proteins and its correlation with fat deposition in Jinhua pigs. Four healthy Jinhua pigs at 45(D45), 90(D90), 150(D150)and 270 (D270) days of age were selected, respectively to measure their body weight and backfat thickness. The ileal mucosa and contents were collected. The expression of fatty acid binding protein genes in the ileal mucosa was measured by fluorescent quantitative PCR, and ileal microbiota were analyzed using high-throughput sequencing based on 16S rRNA gene. The results showed:1) The weight and backfat thickness of Jinhua pigs increased significantly with age increasing (P<0.05); 2) The expression of fatty acid binding protein genes FABP1, FABP2 and FABP4 in the ileal mucosa showed significant increase with age increasing (P<0.05); 3) Firmicutes and Bacteroidetes were the predominant phyla in the ileum of Jinhua pigs. At the genus level, Clostridium sensu stricto 1, Lactobacillus,Terrisporobacter, Romboutsia and Streptococcus were the dominant bacteria; With age increasing, the relative abunance of Clostridium sensu stricto 1 and Streptococcus increased at first and then decreased while Lactobacillus, Terrisporobacter and Romboutsia decreased at first and then increased. PCoA analysis showed that there were significantly different clusters at each age. 4) Spearman correlation analysis showed that the correlation coefficients of the expression levels of fatty acid binding proteins FABP1, FABP2 and FABP4 with backfat thickness were 0.480 1, 0.597 9 and 0.795 3, respectively; The correlation coefficients of Epulopiscium and Mycoplasma with fatty acid binding proteins FABP1, FABP2 and FABP4 in the ileum and backfat thickness of Jinhua pigs were 0.38-0.76 and 0.45-0.64, respectively. It is concluded that the developmental changes were observed for the ileal microbiota and the expression of fatty acid binding protein, which were asscociated with fat deposition in Jinhua pigs.

Key words: Jinhua pig, ileal microbiota, fatty acid binding protein, developmental changes, fat deposition

CLC Number:

S828
S852.6

WANG Yuanxia, LIU Xiuting, ZHANG Xiaojun, XIANG Yun, XU E, LÜ Wentao, YANG Hua, XIAO Yingping. The Developmental Changes of Ileal Microbiota and Fatty Acid Binding Proteins and Its Correlation with Fat Deposition in Jinhua Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 723-732.

References 24

[1]	WANG X F,TASI T,DENG F L,et al.Longitudinal investigation of the swine gut microbiome from birth to market reveals stage and growth performance associated bacteria[J].Microbiome,2019,7(1):109.
[2]	LI N,HUANG S M,JIANG L L,et al.Differences in the gut microbiota establishment and metabolome characteristics between low-and normal-birth-weight piglets during early-life[J].Front Microbiol,2018,9:1798.
[3]	刘颖.梅山猪不同发育阶段肠道微生物变化及免疫调控机制分析[D].扬州:扬州大学,2018.LIU Y.The dynamics of gut microbime and immune regulatory mechanism analysis of Meishan pigs at different growth stages[D].Yangzhou:Yangzhou University,2018.(in Chinese)
[4]	张丽萍.不同品种猪肠道微生物与体脂、ANGPTL4基因关系的研究[D].成都:四川农业大学,2007.ZHANG L P.The relationship of gut microbiota with body fat ANGPTL4 gene in different breeds of pigs[D].Chengdu:Sichuan Agricultural University,2007.(in Chinese)
[5]	郭秀兰.猪肠道硬壁菌门和拟杆菌门数量的检测及其相对丰度与脂肪沉积的相关性研究[D].成都:四川农业大学,2009.GUO X L.Detection of Firmicutes and Bacteroidetes in the pig gut and the correlation between their abundance and fat deposit[D].Chengdu:Sichuan Agricultural University,2009.(in Chinese)
[6]	FISHER R M,ERIKSSON P,HOFFSTEDT J,et al.Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals[J].Diabetologia,2001,44(10):1268-1273.
[7]	ZHANG Y R,ZHANG J M,REN Y H,et al.Tracing the evolution of fatty acid-binding proteins (FABPs) in organisms with a heterogeneous fat distribution[J].FEBS Open Bio,2020,10(5):861-872.
[8]	ZIMMERMAN A W,VEERKAMP J H.New insights into the structure and function of fatty acid-binding proteins[J].Cell Mol Life Sci,2002,59(7):1096-1116.
[9]	HUANG S M,WU Z H,LI T T,et al.Perturbation of the lipid metabolism and intestinal inflammation in growing pigs with low birth weight is associated with the alterations of gut microbiota[J].Sci Total Environ,2020,719:137382.
[10]	GONG Y J,ZOU X T,XIA W R,et al.Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig[J].Czech J Anim Sci,2019,64(8):332-342.
[11]	XIAO Y P,KONG F L,XIANG Y,et al.Comparative biogeography of the gut microbiome between Jinhua and Landrace pigs[J].Sci Rep,2018,8(1):5985.
[12]	YANG H,XIAO Y P,WANG J J,et al.Core gut microbiota in Jinhua pigs and its correlation with strain,farm and weaning age[J].J Microbiol,2018,56(5):346-355.
[13]	YANG H,XIANG Y,ROBINSON K,et al.Gut microbiota is a major contributor to adiposity in pigs[J].Front Microbiol,2018,9:3045.
[14]	王国富,高树新,张立凡,等.大白猪二脂酰甘油酰基转移酶1和2基因(DGAT1和DGAT2)发育性表达特点及其与背膘厚的关联分析[J].农业生物技术学报,2011,19(4):705-710.WANG G F,GAO S X,ZHANG L F,et al.Developmental expression characteristics of Acyl CoA:Diacylglycerol Acyltransferase Genes 1 and 2(DGAT1 and DGAT2) and their association analysis with backfat thickness in Large White pig[J].Journal of Agricultural Biotechnology,2011,19(4):705-710.(in Chinese)
[15]	WANG J J,CHEN L X,LI D F,et al.Intrauterine growth restriction affects the proteomes of the small intestine,liver,and skeletal muscle in newborn pigs[J].J Nutr,2008,138(1):60-66.
[16]	ISAACSON R,KIM H B.The intestinal microbiome of the pig[J].Anim Health Res Rev,2012,13(1):100-109.
[17]	LOOFT T,ALLEN H K,CANTAREL B L,et al.Bacteria,phages and pigs:the effects of in-feed antibiotics on the microbiome at different gut locations[J].ISME J,2014,8(8):1566-1576.
[18]	YANG H,HUANG X C,FANG S M,et al.Uncovering the composition of microbial community structure and metagenomics among three gut locations in pigs with distinct fatness[J].Sci Rep,2016,6(1):27427.
[19]	GERRITSEN J.The genus Romboutsia:genomic and functional characterization of novel bacteria dedicated to life in the intestinal tract[D].Netherlands:Wageningen University,2015.
[20]	NGUGI D K,MIYAKE S,CAHILL M,et al.Genomic diversification of giant enteric symbionts reflects host dietary lifestyles[J].Proc Natl Acad Sci USA,2017,114(36):E7592-E7601.
[21]	PARK S K,KIM M S,ROH S W,et al.Blautia stercoris sp. nov.,isolated from human faeces[J].Int J Syst Evol Microbiol,2012,62(Pt 4):776-779.
[22]	MATHUR R,BARLOW G M.Obesity and the microbiome[J].Exp Rev Gastroenterol Hepatol,2015,9(8):1087-1099.
[23]	HOOPER L V,WONG M H,THELIN A,et al.Molecular analysis of commensal host-microbial relationships in the intestine[J].Science,2001,291(5505):881-884.
[24]	DUCA F A,SAKAR Y,LEPAGE P,et al.Replication of obesity and associated signaling pathways through transfer of microbiota from obese-prone rats[J].Diabetes,2014,63(5):1624-1636.

The Developmental Changes of Ileal Microbiota and Fatty Acid Binding Proteins and Its Correlation with Fat Deposition in Jinhua Pigs

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