1株E亚群禽白血病病毒的分离鉴定及遗传进化分析

doi:10.11843/j.issn.0366-6964.2022.09.025

Abstract

Abstract: Avian leukemia (leukosis) virus subgroup E (ALV-E) refers to endogenous retroviral genomic DNA or fragments present in chicken chromosomes. ALV-E with transcriptional activity will not only have a negative impact on chicken production performance (body weight and egg production), but also interfere with the differential diagnosis of exogenous ALV at the antibody level. In order to isolate and identify an avian leukosis virus RT-PCR positive disease material in a chicken farm in Heilongjiang Province and analyze its genome characteristics and genetic evolution, the virus culture was identified and analyzed by molecular biology, virus morphology and whole genome sequencing. Results showed that the virus could be stably subcultured to the nine generation in CEF cells. Virus particles with approximate spherical shape, diameter of about 80 nm, capsule and fiber structure could be observed using electron microscopy. It was named HLJE2020 strain. Sequence analysis showed that gag and pol genes in the whole genome sequences were relatively conserved. LTR and env genes belong to the same evolutionary branch with ALV-E, while gp85 gene had high homology with ALV-E and ALV-B. Genetic evolution analysis combined with the analysis results of RDPv.4 and SimPlot software showed that there was a separate branch between ALV-E and ALV-B. It is speculated that the gp85 gene of this virus may be recombined with subgroup E AF229 and subgroup B SDAU09C1. This study can provide data for understanding the genetic evolution of avian leukemia virus genome, and provide references and basis for ALV prevention and control.

Key words: avian leukemia virus, isolation and identification, genome analysis, strain of recombinant subgroup E

CLC Number:

S852.659.3

WANG Meng, ZHANG Senhao, ZHANG Liu, LI Jiaxuan, WANG Xiaona, CUI Wen, JIANG Yanping, ZHOU Han, WANG Li, QIAO Xinyuan, LI Yijing, TANG Lijie. Isolation,Identification and Genetic Evolution Analysis of a Subgroup E Avian Leukemia Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3107-3120.

References 32

[1]	LI H J, WANG P K, LIN L L, et al. The emergence of the infection of subgroup J avian leucosis virus escalated the tumour incidence in commercial yellow chickens in Southern China in recent years[J]. Transbound Emerg Dis, 2019, 66(1):312-316.
[2]	PAYNE L N, NAIR V. The long view:40 years of avian leukosis research[J]. Avian Pathol, 2012, 41(1):11-19.
[3]	马美哥. 我国部分地方品种鸡和进口白羽肉种鸡ALV-J的分子流行病学调查及致病性研究[D]. 石河子:石河子大学, 2019.MA M G. Molecular epidemiological investigation and pathogenicity of subgroup J avian leukosis in part local breeders and imported broiler breeders[D]. Shihezi:Shihezi University, 2019. (in Chinese)
[4]	ZHAO Z J, RAO M Z, LIAO M, et al. Phylogenetic analysis and pathogenicity assessment of the emerging recombinant subgroup K of Avian Leukosis virus in South China[J]. Viruses, 2018, 10(4):194.
[5]	SUN T, WANG X M, HAN W, et al. Complete genome sequence of a novel recombinant avian Leukosis virus isolated from a three-yellow chicken[J]. Arch Virol, 2020, 165(11):2615-2618.
[6]	WANG P K, YANG Y L, LIN L L, et al. Complete genome sequencing and characterization revealed a recombinant subgroup B isolate of avian Leukosis virus with a subgroup J-like U3 region[J]. Virus Genes, 2017, 53(6):927-930.
[7]	LI X Y, YU Y, MA M G, et al. Molecular characteristic and pathogenicity analysis of a novel multiple recombinant ALV-K strain[J]. Vet Microbiol, 2021, 260:109184.
[8]	毕研丽, 庄金秋, 王金良, 等. 双重PCR检测禽源生物制品中禽网状内皮组织增生症病毒和禽白血病病毒[J]. 畜牧与兽医, 2014, 46(4):74-78.BI Y L, ZHUANG J Q, WANG J L, et al. Detection of avian reticuloendothelial hyperplasia virus and avian leukemia virus in avian biological products by dual PCR[J]. Animal Husbandry & Veterinary Medicine, 2014, 46(4):74-78. (in Chinese)
[9]	李凯, 高宏雷, 高立, 等. 禽网状内皮组织增生病病毒TaqMan探针荧光定量PCR检测方法的建立及应用[J]. 中国兽医科学, 2010, 40(11):1137-1141.LI K, GAO H L, GAO L, et al. Development and application of a TaqMan real-time PCR assay for detection of reticuloendotheliosis virus[J]. Chinese Veterinary Science, 2010, 40(11):1137-1141. (in Chinese)
[10]	金娟, 李亚杰, 鲍恩东. 1株禽传染性支气管炎病毒的分离鉴定及其实时荧光定量PCR检测方法的建立[J]. 畜牧与兽医, 2020, 52(1):115-121.JIN J, LI Y J, BAO E D, et al. Isolation and identification of a strain of avian infectious bronchitis virus and establishment of real-time fluorescent quantitative PCR for the strain[J]. Animal Husbandry & Veterinary Medicine, 2020, 52(1):115-121. (in Chinese)
[11]	屈素洁, 施开创, 邹联斌, 等. 新城疫病毒和传染性支气管炎病毒双重荧光定量RT-PCR检测方法的建立[J]. 中国预防兽医学报, 2014, 36(11):867-871.QU S J, SHI K C, ZOU L B, et al. Development of a dultiplex real-time RT-PCR assay for detection of Newcastle disease virus and avian infectious bronchitis viruses[J]. Chinese Journal of Preventive Veterinary Medicine, 2014, 36(11):867-871. (in Chinese)
[12]	王楷宬, 王素春, 朱琳, 等. 禽流感病毒实时荧光定量RT-PCR检测方法的建立[J]. 中国动物检疫, 2019, 36(4):64-69.WANG K C, WANG S C, ZHU L, et al. Establishment of real-time RT-PCR for detection of avian influenza virus[J]. China Animal Health Inspection, 2019, 36(4):64-69. (in Chinese)
[13]	钟丽, 孙美玉, 高立, 等. 禽呼肠孤病毒TaqMan实时荧光RT-PCR方法的建立及病毒组织分布比较研究[J]. 中国动物传染病学报, 2017, 25(2):56-60.ZHONG L, SUN M Y, GAO L, et al. Development and application of a TaqMan RT-PCR assay for detection of avian reoviruses in tissues[J]. Chinese Journal of Animal Infectious Diseases, 2017, 25(2):56-60. (in Chinese)
[14]	张颖. 鸡马立克氏病病毒TaqMan探针双重荧光定量PCR检测方法的建立及应用[D]. 呼和浩特:内蒙古农业大学, 2006.ZHANG Y. Establishment and application of diagnostic test for Marek's disease virus based on TaqMan probe double Fluorescent Quantitative Polymerase-Chain-Reaction (FQ-PCR)[D]. Hohhot:Inner Mongolia Agricultural University, 2006. (in Chinese)
[15]	宋修庆. VP3对鸡贫血病毒复制的影响及核定位信号的作用[D]. 北京:中国农业科学院, 2009.SONG X Q. Effect of chicken anemia virus VP3 on viral replication and function of nuclear location signal[D]. Beijing:Chinese Academy of Agricultural Sciences, 2009. (in Chinese)
[16]	赵妍, 孔聪聪, 张晓敏, 等. 鸡传染性喉气管炎病毒TaqMan real-time PCR检测方法的建立[J]. 中国预防兽医学报, 2012, 34(8):642-646.ZHAO Y, KONG C C, ZHANG X M, et al. Development of a TaqMan real-time PCR for detection of infectious laryngotracheitis virus[J]. Chinese Journal of Preventive Veterinary Medicine, 2012, 34(8):642-646. (in Chinese)
[17]	SMITH L M, BROWN S R, HOWES K, et al. Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus[J]. Virus Res, 1998, 54(1):87-98.
[18]	俞燕, 周生, 李建梅, 等. A、B、J和K亚群禽白血病病毒多重PCR检测方法的建立及应用[J]. 中国预防兽医学报, 2020, 42(5):462-468.YU Y, ZHOU S, LI J M, et al. Establishment and application of multiple PCR for detection of avian leukosis viruses subgroup A, B, J and K[J]. Chinese Journal of Preventive Veterinary Medicine, 2020, 42(5):462-468. (in Chinese)
[19]	MARTIN D P, MURRELL B, GOLDEN M, et al. RDP4:detection and analysis of recombination patterns in virus genomes[J]. Virus Evol, 2015, 1(1):vev003.
[20]	陈茹. 两株A亚群和一株K亚群禽白血病病毒体外增殖及对雏鸡致病性比较[D]. 泰安:山东农业大学, 2018.CHEN R. Comparisons of the proliferation in vitro and the pathogenicity in chickens between two ALV-a strains and one ALV-K strain[D]. Taian:Shandong Agricultural University, 2018. (in Chinese)
[21]	武专昌. 一株B亚型ALV的分离、前病毒全基因组序列分析和感染性克隆的构建[D]. 泰安:山东农业大学, 2012.WU Z C. Isolation, sequence analysis for the complete Proviral genome and construction of infectious clone of a subgroup B ALV[D]. Taian:Shandong Agricultural University, 2012.(in Chinese)
[22]	CAI L M, SHEN Y W, WANG G H, et al. Identification of two novel multiple recombinant avian Leukosis viruses in two different lines of layer chicken[J]. J Gen Virol, 2013, 94(Pt 10):2278-2286.
[23]	SANDELINI K, ESTOLA T. Occurrence of different subgroups of avian Leukosis virus in Finnish poultry[J]. Avian Pathol, 1974, 3(3):159-168.
[24]	MAO Y Q, SU Q, LI J P, et al. Avian Leukosis virus contamination in live vaccines:a retrospective investigation in China[J]. Vet Microbiol, 2020, 246:108712.
[25]	ZHOU D F, XUE J W, ZHANG Y, et al. Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018[J]. Transbound Emerg Dis, 2019, 66(2):622-626.
[26]	LI Q H, WANG P K, LI M, et al. Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity[J]. Vet Microbiol, 2020, 250:108862.
[27]	徐海鹏, 孟凡峰, 董宣, 等. 种蛋中内源性禽白血病病毒的检测和鉴定[J]. 畜牧兽医学报, 2014, 45(8):1317-1323.XU H P, MENG F F, DONG X, et al. Detection and identification of an endogenous subgroup E avian leukosis virus in a chicken breeder embryo[J]. Acta Veterinaria et Zootechnica Sinica, 2014, 45(8):1317-1323. (in Chinese)
[28]	DONG X, MENG F F, HU T, et al. Dynamic co-evolution and interaction of avian Leukosis virus genetic variants and host immune responses[J]. Front Microbiol, 2017, 8:1168.
[29]	WANG P K, NIU J R, XUE C, et al. Two novel recombinant avian leukosis virus isolates from Luxi gamecock chickens[J]. Arch Virol, 2020, 165(12):2877-2881.
[30]	FEDERSPIEL M J. Reverse engineering provides insights on the evolution of subgroups A to E avian sarcoma and Leukosis virus receptor specificity[J]. Viruses, 2019, 11(6):497.
[31]	VENUGOPAL K, SMITH L M, HOWES K, et al. Antigenic variants of J subgroup avian leukosis virus:sequence analysis reveals multiple changes in the env gene[J]. J Gen Virol, 1998, 79(Pt 4):757-766.
[32]	HOLMEN S L, MELDER D C, FEDERSPIEL M J. Identification of key residues in subgroup A avian leukosis virus envelope determining receptor binding affinity and infectivity of cells expressing chicken or quail Tva receptor[J]. J Virol, 2001, 75(2):726-737.

Isolation,Identification and Genetic Evolution Analysis of a Subgroup E Avian Leukemia Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 32

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHENG Rui, LIU Zishi, ZHANG Kangyou, YAN Yong, WEI Ling, ZEREN Wengmu, DINGZE Demi, HUANG Jianjun, WANG Li, WEI Yong. Isolation, Identification and Biological Characterization of Colletotrichum jasminigenum in Stems of Peanuts [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2206-2213.
[2]	TIAN Rui, XU Sixiang, XIE Feng, LIU Guangjin, WANG Gang, LI Qingxia, DAI Lei, XIE Guoxin, ZHANG Qiongwen, LU Yajing, WANG Guangwen, WANG Jinxiu, ZHANG Wei. Bioinformatics Analysis of the Genome of Clostridium perfringens Isolated from Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1707-1715.
[3]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[4]	LIU Xinhuan, YUN Jialei, MAO Li, LI Jizong, HAO Fei, HE Miaofeng, YANG Leilei, ZHANG Wenwen, CHENG Zilong, SUN Min, LIU Maojun, WANG Shaohui, BAI Juan, LI Wenliang. Isolation, Identification, Virulence Genes and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheal Goat and Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3445-3454.
[5]	WANG Jiali, ZHOU Ning, CHEN Xi, YUE Hua, TANG Cheng. Isolation, Identification and Pathogenicity of Canine Adenovirus Type 2 Strain with Natural Deletion in E3 Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2982-2990.
[6]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.
[7]	LIU Jiankui, XU Ye, LIU Chen, YU Hui, YANG Yuan, HE Le, LI Jiarui, DAN Huijuan, DAI Ailing, YANG Xiaoyan, WEI Chunhua. Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in Fujian Province from 2017 to 2021 based on Whole Genome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1579-1589.
[8]	ZHANG Ao, TAN Bin, LIU Kexin, LIU Jiali, ZHANG Shuqin. Genomic Characterization Analysis of a H1N1 Subtype Swine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4866-4871.
[9]	LI Chang, ZHOU Ping, WEN Ping, YANG Keli, LIU Wei, GUO Rui, LIU Zewen, YUAN Fangyan, GAO Ting, SONG Haofei, TIAN Yongxiang, ZHOU Danna. Investigation of the Genome-wide Sequence and Immunogenicity of Capsid Protein in Mice for a Porcine Circovirus 2d Genotype Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4289-4299.
[10]	XU Lifeng, JIA Chenyu, CHEN Fuzai, FANG Mengyuan, LIU Xiaodan, CHEN Jilong, LI Xunliang. Isolation, Identification and Whole Genome Analysis of a Mycoplasma synoviae Strain from Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2663-2676.
[11]	LI Huaming, XIANG Wei, LU Wenbing, LIU Feng, LEI Liancheng, ZHANG Fuxian. Pathogenicity and Drug Sensitivity Analysis of a Porcine Klebsiella pneumoniae Type ST-35 [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4356-4366.
[12]	HE Jian, XING Xiaoyong, WU Xiaochun, WEN Fengqin, ZHANG Yangyang, ZHANG Shengying, LIU Jia, BAO Shijun. Isolation and Identification of a Strain of Pseudomonas aeruginosa from Forest Musk and Analysis of the Whole Genome Sequence [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2569-2578.
[13]	HUANG Chengyu, XU Dequan, FENG Zhe, ZHOU Ling, LIU Min. The Addition of Melatonin Reduces the Oxidative Damage of Pig Spermatogonial Stem Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 1880-1890.
[14]	WANG Jia, HUA Lizhong, LIU Beibei, ZHANG Lei, YUAN Ting, GAN Yuan, WEI Yanna, SHAO Guoqing, FENG Zhixin, XIONG Qiyan. Isolation, Identification and Pathogenicity of a Mycoplasma hyorhinis Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3185-3193.
[15]	ZHANG Yanan, LI Yafei, CHEN Rujia, YU Bo, PENG Shan, LI Ting, PU Ling, XU Jinge. Resistance and Virulence Analysis of Type A: L1 ST128 Pasteurella multocida from Ducks [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2852-2863.