Abstract: To scientifically select and use brucellosis antibody detection method, and promote the standardization of brucellosis diagnostic reagents. In this study, the sensitivities of four methods, which included Brucella fluorescence polarisation assay (FPA) antibody test kit, animal brucellosis competitive ELISA (cELISA) antibody test kit, bovine brucellosis indirect ELISA (iELISA) antibody test kit and the improved micro complement fixation test (mCFT) developed by the National/OIE Reference Laboratory for Brucellosis, were determined with the brucellosis positive serum standard. The sensitivity and specificity of the four methods were assessed by the detection serum samples with known background. At the same time, the clinical bovine serum samples were detected by the above four methods, and the coincidence rates were compared. The results showed that the sensitivities of the four methods were the same. The detection results of brucellosis positive serum standard diluted 1∶20 (50 IU·mL^-1) was positive, and the detection results of brucellosis positive serum standard diluted 1∶40 (25 IU·mL^-1) was negative. The sensitivity of FPA, cELISA, iELISA and mCFT were 97.14%, 100.00%, 100.00%, 98.57%, respectively. The specificity of the four methods were 96.34%, 95.12%, 97.56%, 100.00%, respectively. By detecting the 315 clinical samples, the result showed that the coincidence rates of different methods were higher than 90.00%. According to the confirm result of mCFT, the coincidence rates of iELISA, FPA and cELISA were 97.14%, 96.83% and 92.70%, respectively. Compared with iELISA, the coincidence rates of FPA and cELISA were 95.24% and 93.65%, respectively. Compared with cELISA, the coincidence rate of FPA was 91.43%. The identical degree of iELISA, FPA and mCFT was the highest, while the identical degree of cELISA and the other three methods was slightly lower.

Key words: Brucella, antibody detection, method comparison