微囊藻毒素-LR对猪体外成熟卵母细胞氧化损伤与凋亡的影响

doi:10.11843/j.issn.0366-6964.2021.010

Abstract

Abstract: The aim of this study was to investigate the toxic damage effect of microcystin-LR (MC-LR) on porcine oocytes in vitro and its potential mechanism. The GV oocytes were collected from porcine ovaries obtained from a local slaughterhouse and randomly assigned into 4 groups, 0, 20, 40 and 60 μg·mL^-1 MC-LR were added into maturation medium in vitro, respectively. Then, the effects of MC-LR on the first polar body (PbI) extrusion, spindle structure, reactive oxygen species (ROS), membrane mucin V(Annexin V) and glutathione peroxidase (GSH-Px) activity were examined, respectively. The mRNA expression of oxidative stress and apoptosis related genes (SOD1, SOD2, CAT, GSH-Px, Bax and Bcl2) were also analyzed by RT-qPCR, and three replicates were performed for each experiment. The result showed that the PbI extrusion rate of porcine oocytes decreased in a concentration-dependent manner after MC-LR treatment. When the MC-LR concentration reached more than 40 μg·mL^-1, the oocyte maturation rate extremely significantly decreased(P<0.01), and the spindle structure abnormality rate also extremely significantly increased(P<0.001). Further studies showed that, after MC-LR treatment, ROS levels in oocytes were extremely significantly increased (P<0.01), while GSH-Px activity was extremely significantly decreased (P<0.01), accompanied by extremely significant down-regulation of antioxidant related genes SOD1, CAT and GSH-Px mRNA expressions(P<0.01). Apoptosis detection showed that MC-LR treatment could extremely significantly increase the early apoptosis rate of oocytes (P<0.01), and the ratio of Bax/Bcl2 was extremely significantly increased (P<0.001). The results indicate that MC-LR can induce abnormal spindle structure of porcine oocytes, lead to oxidative stress and apoptosis, and eventually result in a decline of porcine oocyte maturation ability.

Key words: microcystin-LR, porcine, oocyte, spindle, oxidative stress, apoptosis

CLC Number:

S828.3

SHI Fengyao, LI Wenhui, ZHAO Hongyu, WANG Yang, ZHUANG Ruixue, RUI Rong, JU Shiqiang. Effects of MC-LR on Oxidative Stress and Apoptosis of Porcine Oocytes in Vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 98-106.

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Effects of MC-LR on Oxidative Stress and Apoptosis of Porcine Oocytes in Vitro

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