类乌齐牦牛全基因组RNA编辑位点预测及剖析

doi:10.11843/j.issn.0366-6964.2021.009

Abstract

Abstract: The study aimed to reveal the variation of transcription products at different RNA expression levels among yak tissues, and provide candidate genes for yak tissue differentiation, systematic genetic regulation and other studies. The cerebrum, cerebellum, buttock fat and buttock muscle tissues of 3 Leiwuqi Yaks with 4.5 years old were collected as experimental material. The expression products of each tissue were scaned through the Illumina 4000 sequencing platform, the SPRINT and JACUSA softwares were used to screen differential RNA editing sites, the classification, annotations and mutation risk assessment of RNA editing sites were analyzed. A total of 24 784 RNA editing sites were found, of which 4 015 sites with editing events were observed in the 4 tissues. The RNA editing sites in the 4 tissues were mainly A-G and T-C editing types. It was found that one of the high-risk editing sites was located in the SON gene, which stopped the translation of the gene prematurely, and caused problems in the binding of RNA to DNA at certain sites in the tissue. The types and distribution of RNA editing sites in cerebrum, cerebellum, buttock fat and buttock muscle tissues of Leiwuqi yaks were predicted and analyzed in this study, which provided a new reference for further study on regulatory genes in yak tissue differentiation and growth.

Key words: Leiwuqi yaks, RNA editing site, SPRINT, JACUSA

CLC Number:

S823.85

WANG Jiabo, SHU Tao, CHAI Zhixin, WANG Jikun, WANG Hui, WU Zhijuan, TANG You, ZHONG Jincheng, JI Qiumei. Prediction and Analysis of RNA Editing Sites in the Whole Genome of Leiwuqi Yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 88-97.

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Prediction and Analysis of RNA Editing Sites in the Whole Genome of Leiwuqi Yaks

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