Apelin-13对小鼠血脂代谢的影响及其机制研究

doi:10.11843/j.issn.0366-6964.2020.09.018

Abstract

Abstract: The objective of this study was to explore the physiological mechanism of Apelin-13 regulating blood lipid metabolism in mice. Ninety-six mice with similar body weight were randomly divided into 4 treatments, with 4 replicates each. In a 14-day trial period, the mice of the control group and 3 treatment groups were injected subcutaneously with 100 μL 0.9% normal saline, 10, 20 and 50 μg·kg^-1 Apelin-13 each day, respectively. The growth performance, serum Apelin-13 and blood lipids contents were measured. The transcriptomic profiling of liver tissues was sequenced using Illumina Novaseq technology. The results showed that compared to control group, the serum Apelin-13 and high-density lipoprotein cholesterol contents were increased extremely significantly (P<0.01) and triglycerides contents were decreased significantly (P<0.05) in treatment groups. A total of 340 up-regulated and 350 down-regulation differentially expressed genes (DEGs) were identified among treatment groups and control group. The most significant enriched signaling pathway was retinol metabolism, in which cytochrome P450 (Cyp) and uridine diphosphate glucuronidase (Ugt) superfamily genes were mostly up-regulated. The results of this study indicated that Apelin-13 might regulate blood lipid metabolism in mice by regulating the expression of Cyp and Ugt superfamily genes in the retinol metabolism signaling pathway.

Key words: mice, Apelin-13, retinol metabolism, DEGs, liver, RNA-seq

CLC Number:

Q493.5

ZHANG Min, LI Jianlei, ZHU Yuting, CHEN Yu, LIN Ze, WU Qiong, LI Yan, SUN Yanfa. Study of Apelin-13 on Blood Lipid Metabolism and Its Mechanism in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2207-2215.

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Study of Apelin-13 on Blood Lipid Metabolism and Its Mechanism in Mice

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