不同营养水平日粮对陕北白绒山羊生长性能和小肠组织SLC7A7、SLC3A1及SLC15A1 mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2020.07.018

Abstract

Abstract: The aim of this study was to investigate the effects of diets with different nutrition levels on the growth performance and the mRNA expression of SLC7A7, SLC3A1 and SLC15A1 in intestinal tissues of 2-6 months old weaned Shaanbei white cashmere goats. Thirty-six healthy female Shaanbei white cashmere goats with age (60±1.60) d and body weight (10.73±1.03) kg were randomly divided into 4 groups and fed with 4 test diets, which digestive energy and crude protein were 85%, 100%, 115% and 130% of the standard diet, respectively. The nutrition level of standard diet formulated with reference to the NY/T816-2004. Goats were weighted at 120 and 180 d. One goat (a total of 12 goats) was slaughtered in each replicate. The upper middle part of the duodenum, the middle part of the jejunum and the ileum end tissue samples were collected. The mRNA expression levels of SLC7A7,SLC3A1 and SLC15A1 in the sample tissues were detected by real-time fluorescent quantitative PCR. The results showed that:1) In the first stage (60-120 d), the average daily gain of the 115% level group was significantly higher than those of other groups (P<0.05); In the second stage (121-180 d), the average daily gain of the 115% level group was significantly higher than 85% and 100% levels groups (P<0.05), but there was no significant difference compared to 130% level group (P>0.05). The dry matter intake of lambs in the 115% level group in the two stages were extremely significantly higher than those of other groups (P<0.01). The feed to gain ratio of 115% level group was significantly lower than those of 85% and 100% level groups (P<0.05). 2) With the same nutrition level, the mRNA expression levels of SLC7A7 and SLC15A1 were all in order of ileum>jejunum>duodenum; 3) The mRNA expression of SLC7A7, SLC3A1 and SLC15A1 in small intestines increased first and then decreased with the increase of dietary nutrient level, and the 115% level showed the highest expression. The mRNA expression of SLC7A7 and SLC15A1 in the 115% group was significantly higher than those of the the other three groups (P<0.05). The mRNA expression of SLC3A1 in the 115% group was significantly higher than those of the 85% and 130% groups (P<0.05). In this experiment, goats at age of 2-6 month-old fed the diet with 115% digestible energy and crude protein of the standard showed better growth performance, and the mRNA expression of SLC7A7, SLC3A1 and SLC15A1 in the small intestine was higher than those of the other groups.

Key words: Shaanbei white cashmere goats, dietary nutrition level, growth performance, SLC7A7, SLC3A1, SLC15A1

CLC Number:

S827

QU Xingmei, XING Xiaonan, XUE Fulai, JIN Miaohan, PENG Xiaokun, LI Jiateng, ZHANG Enping. Effects of Diets with Different Nutrition Levels on Growth Performance and Small Intestine Tissue SLC7A7、SLC3A1 and SLC15A1 mRNA Expression in Shaanbei White Cashmere Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1656-1665.

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Effects of Diets with Different Nutrition Levels on Growth Performance and Small Intestine Tissue SLC7A7、SLC3A1 and SLC15A1 mRNA Expression in Shaanbei White Cashmere Goats

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