不同粪菌悬液处理方式对猪粪菌移植过程中细菌活性及活菌组成的影响

doi:10.11843/j.issn.0366-6964.2020.05.015

Abstract

Abstract: The purpose of this study is to explore the influence of different treatments on the microbial activity and live bacteria composition during the preparation of faecal bacteria suspension, so as to provide reference for optimizing the application of faecal microbiota transplantation(FMT) on pig. Seven experimental groups were set up in this study:microbial suspension from fresh feces by static settlement (FS), microbial suspension from fresh feces by static settlement and stored at -80℃ for 1 week (FFS), microbial suspension from fresh feces by centrifugation (FC), microbial suspension from fresh feces by centrifugation and stored at -80℃ for 1 week (FFC), microbial suspension from feces frozen in nitrogen by static settlement (NS), microbial suspension from feces treated with glycerin and frozen in nitrogen for 24 h by static settlement (GNS), and microbial suspension from feces frozen in nitrogen by static settlement and stored at -80℃ for 1 week (NFS). All suspensions were spread on plates and cultivated at 37℃ anaerobic incubator for 48 h. The colonies growing were counted. In addition, 87 single colonies were randomly selected from the four groups of FS, FFS, FC and FFC and purified for full-length sequencing of 16S rRNA gene. The results showed that there were significant differences in bacteria activity when preparing suspensions from fresh feces by static settlement and centrifugation methods, and the activity of faecal bacteria in the centrifugation group was significantly lower than that in the static settlement group (P<0.05). However, there was no significant influence of suspensions from frozen feces on the activity of faecal bacteria regardless of static settlement or centrifugation (P>0.05). Whether the suspensions were prepared by static settlement or centrifugation, no significant impact was found on the activity of faecal bacteria either from fresh or frozen feces (P>0.05),nor did on the live bacteria composition. When microbial suspensions were prepared by static settlement, there were no significant difference in faecal bacteria activity (P>0.05) among FS, NS, GNS and NFS groups. The above results indicate that when preparing faecal microbial suspensions by static settlement, ultra-low temperature cryopreservation has no significant effect on the activity and composition of faecal bacteria. The present research provided a useful suggestion for the application of long distance and large size faecal microbiota transplantation technology in pig production in the future.

Key words: faecal microbiota transplantation, gut microbiota, bacterial culture, activity of faecal microbiota, pig

CLC Number:

S828

LIU Min, WU Jinyuan, CHEN Congying. Effects of Different Treatments of Faecal Microbial Suspensions on the Activity and Composition of Live Bacteria in Faecal Microbiota Transplantation in Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1040-1048.

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Effects of Different Treatments of Faecal Microbial Suspensions on the Activity and Composition of Live Bacteria in Faecal Microbiota Transplantation in Pigs

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