CRISPR/Cas9基因编辑技术在重要猪病毒病防控中的研究与应用

doi:10.11843/j.issn.0366-6964.2020.05.006

Abstract

Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR) is an acquired immune system that exists widely in archaea and bacteria, mediated by RNA and assisted by Cas protein. It has been found that the CRISPR/Cas9 was the most widely used CRISPR system. This review mainly summarizes the basic principle and research progress of the CRISPR/Cas9 system, and highlights its application in the prevention and control of important swine virus diseases, including host modification and virus modification. This technology provides a powerful tool for the study of virus pathogenic mechanism, next generation vaccine development and disease resistance breeding. It has a far-reaching impact on the control of the diseases.

Key words: CRISPR/Cas9, prevention and control of swine disease, next generation vaccine, disease resistance breeding

CLC Number:

DAI Xueyu, ZHANG Qianyi, XU Lu, ZHAO Qizu, WANG Qin, XIA Yingju. Research Progress and Application of CRISPR/Cas9 Gene Editing Technology in Prevention and Control of Important Swine Virus Diseases[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 943-951.

References 64

[1]	HORVATH P, BARRANGOU R. CRISPR/Cas, the immune system of bacteria and archaea[J]. Science, 2010, 327(5962):167-170.
[2]	杨发誉, 葛香连, 谷峰. 新型靶向基因组编辑技术研究进展[J]. 中国生物工程杂志, 2014, 34(2):98-103.YANG F Y, GE X L, GU F. Progress of next-generation targeted gene-editing techniques[J]. China Biotechnology, 2014, 34(2):98-103. (in Chinese)
[3]	EBINA H, MISAWA N, KANEMURA Y, et al. Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus[J]. Sci Rep, 2013, 3:2510.
[4]	HU W H, KAMINSKI R, YANG F, et al. RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection[J]. Proc Natl Acad Sci U S A, 2014, 111(31):11461-11466.
[5]	李晓开, 龙科任, 麦苗苗, 等. CRISPR-Cas9技术的原理及其在猪研究中的应用[J]. 生命科学, 2018, 30(6):690-700.LI X K, LONG K R, MAI M M, et al. The principle of CRISPR-Cas9 technology and its application in pig research[J]. Chinese Bulletin of Life Sciences, 2018, 30(6):690-700. (in Chinese)
[6]	INUI M, MIYADO M, IGARASHI M, et al. Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system[J]. Sci Rep, 2015, 4:5396.
[7]	HECKL D, KOWALCZYK M S, YUDOVICH D, et al. Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing[J]. Nat Biotechnol, 2014, 32(9):941-946.
[8]	SOREK R, KUNIN V, HUGENHOLTZ P. CRISPR-a widespread system that provides acquired resistance against phages in bacteria and archaea[J]. Nat Rev Microbiol, 2008, 6(3):181-186.
[9]	MAKAROVA K S, ARAVIND L, WOLF Y I, et al. Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems[J]. Biol Direct, 2011, 6:38.
[10]	MAKAROVA K S, HAFT D H, BARRANGOU R, et al. Evolution and classification of the CRISPR-Cas systems[J]. Nat Rev Microbiol, 2011, 9(6):467-477.
[11]	MAKAROVA K S, WOLF Y I, ALKHNBASHI O S, et al. An updated evolutionary classification of CRISPR-Cas systems[J]. Nat Rev Microbiol, 2015, 13(11):722-736.
[12]	MAKAROVA K S, WOLF Y I, KOONIN E V. Classification and nomenclature of CRISPR-cas systems:where from here?[J]. CRISPR J, 2018, 1(5):325-336.
[13]	ISHINO Y, SHINAGAWA H, MAKINO K, et al. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product[J]. J Bacteriol, 1987, 169(12):5429-5433.
[14]	JANSEN R, VAN EMBDEN J D A, GAASTRA W, et al. Identification of genes that are associated with DNA repeats in prokaryotes[J]. Mol Microbiol, 2002, 43(6):1565-1575.
[15]	BOLOTIN A, QUINQUIS B, SOROKIN A, et al. Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin[J]. Microbiology, 2005, 151(8):2551-2561.
[16]	BARRANGOU R, FREMAUX C, DEVEAU H, et al. CRISPR provides acquired resistance against viruses in prokaryotes[J]. Science, 2007, 315(5819):1709-1712.
[17]	MARRAFFINI L A, SONTHEIMER E J. CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA[J]. Science, 2008, 322(5909):1843-1845.
[18]	RAN F A, HSU P D, WRIGHT J, et al. Genome engineering using the CRISPR-Cas9 system[J]. Nat Protoc, 2013, 8(11):2281-2308.
[19]	JINEK M, EAST A, CHENG A, et al. RNA-programmed genome editing in human cells[J]. eLife, 2013, 2:e00471.
[20]	HSU P D, LANDER E S, ZHANG F. Development and applications of CRISPR-Cas9 for genome engineering[J]. Cell, 2014, 157(6):1262-1278.
[21]	JINEK M, CHYLINSKI K, FONFARA I, et al. A programmable dual-RNA-Guided DNA endonuclease in adaptive bacterial immunity[J]. Science, 2012, 337(6096):816-821.
[22]	LIEBER M R. The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway[J]. Ann Rev Biochem, 2010, 79:181-211.
[23]	RUDIN N, SUGARMAN E, HABER J E. Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae[J]. Genetics, 1989, 122(3):519-534.
[24]	JAO L E, WENTE S R, CHEN W B. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system[J]. Proc Natl Acad Sci U S A, 2013, 110(34):13904-13909.
[25]	PORT F, CHEN H M, LEE T, et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila[J]. Proc Natl Acad Sci U S A, 2014, 111(29):E2967-E2976.
[26]	CONG L, RAN F A, COX D, et al. Multiplex genome engineering using CRISPR/Cas systems[J]. Science, 2013, 339(6121):819-823.
[27]	MALI P, YANG L H, ESVELT K M, et al. RNA-guided human genome engineering via Cas9[J]. Science, 2013, 339(6121):823-826.
[28]	XU L, WANG J, LIU Y L, et al. CRISPR-edited stem cells in a patient with HIV and acute lymphocytic leukemia[J]. N Engl J Med, 2019, 381(13):1240-1247.
[29]	FAO. World agriculture:towards 2015/2030[DB/OL].[2020-01-18] http://www.fao.org/3/Y4252E/y4252e05b.htm#P3_3.
[30]	杜金芳, 王慧. 转基因猪的研究进展[J]. 猪业科学, 2009, 26(9):82-84.DU J F, WANG H. Research progress of transgenic swine[J]. Swine Industry Science, 2009, 26(9):82-84. (in Chinese)
[31]	HAI T, TENG F, GUO R F, et al. One-step generation of knockout pigs by zygote injection of CRISPR/Cas system[J]. Cell Res, 2014, 24(3):372-375.
[32]	WANG K P, JIN Q, RUAN D G, et al. Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing[J]. Genome Res, 2017, 27(12):2061-2071.
[33]	YANG H Q, WU Z F. Genome editing of pigs for agriculture and biomedicine[J]. Front Genet, 2018, 9:360.
[34]	NIU D, WEI H J, LIN L, et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9[J]. Science, 2017, 357(6357):1303-1307.
[35]	HAUSCHILD J, PETERSEN B, SANTIAGO Y, et al. Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases[J]. Proc Natl Acad Sci U S A, 2011, 108(29):12013-12017.
[36]	CARLSON D F, TAN W F, LILLICO S G, et al. Efficient TALEN-mediated gene knockout in livestock[J]. Proc Natl Acad Sci U S A, 2012, 109(43):17382-17387.
[37]	WHITWORTH K M, LEE K, BENNE J A, et al. Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos[J]. Biol Reprod, 2014, 91(3):78.
[38]	PRATHER R S, WELLS K D, WHITWORTH K M, et al. Knockout of maternal CD163 protects fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV)[J]. Sci Rep, 2017, 7(1):13371.
[39]	BURKARD C, LILLICO S G, REID E, et al. Precision engineering for PRRSV resistance in pigs:macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function[J]. PLoS Pathog, 2017, 13(2):e1006206.
[40]	CHEN J Y, WANG H T, BAI J H, et al. Generation of pigs resistant to highly pathogenic-porcine reproductive and respiratory syndrome virus through gene editing of CD163[J]. Int J Biol Sci, 2019, 15(2):481-492.
[41]	XIE Z C, PANG D X, YUAN H M, et al. Genetically modified pigs are protected from classical swine fever virus[J]. PLoS Pathog, 2018, 14(12):e1007193.
[42]	XU D, DU Q, HAN C, et al. P53 signaling modulation of cell cycle arrest and viral replication in porcine circovirus type 2 infection cells[J]. Vet Res, 2016, 47:120.
[43]	LI P F, ZHANG X L, CAO W J, et al. RIG-I is responsible for activation of type I interferon pathway in Seneca Valley virus-infected porcine cells to suppress viral replication[J]. Virol J, 2018, 15(1):162.
[44]	SUI C, JIANG D D, WU X J, et al. CRISPR-Cas9 mediated RNase L knockout regulates cellular function of PK-15 cells and increases PRV replication[J]. BioMed Res Int, 2019, 2019:7398208.
[45]	HVBNER A, PETERSEN B, KEIL G M, et al. Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L)[J]. Sci Rep, 2018, 8:1449.
[46]	LIANG X, SUN L Q, YU T, et al. A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging pseudorabies virus[J]. Sci Rep, 2016, 6:19176.
[47]	BORCA M V, HOLINKA L G, BERGGREN K A, et al. CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses[J]. Sci Rep, 2018, 8(1):3154.
[48]	TANG Y D, LIU J T, WANG T T, et al. CRISPR/Cas9-mediated multiple single guide RNAs potently abrogate pseudorabies virus replication[J]. Arch Virol, 2017, 162(12):3881-3886.
[49]	PENG Q, FANG L R, DING Z, et al. Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology[J]. J Virol Methods, 2020, 276:113772.
[50]	TONG G Z, ZHOU Y J, HAO X F, et al. Highly pathogenic porcine reproductive and respiratory syndrome, China[J]. Emerg Infect Dis, 2007, 13(9):1434-1436.
[51]	赵鸿远, 彭金美, 安同庆, 等. 猪伪狂犬病病毒变异株的分离鉴定及其gE基因的分子特征[J]. 中国预防兽医学报, 2014, 36(7):506-509.ZHAO H Y, PENG J M, AN T Q, et al.Identification of glycoprotein E characteristic in pseudorabies virus variants from swine[J]. Chinese Journal of Preventive Veterinary Medicine, 2014, 36(7):506-509.(in Chinese)
[52]	施标, 董世娟, 朱于敏, 等. 中国猪流行性腹泻病毒分子流行病学研究进展[J]. 中国农业科学, 2013, 46(20):4362-4369.SHI B, DONG S J, ZHU Y M, et al. Advances in study of molecular epidemiology of porcine epidemic diarrhea virus in China[J]. Scientia Agricultura Sinica, 2013, 46(20):4362-4369. (in Chinese)
[53]	PALINSKI R, PIÑEYRO P, SHANG P C, et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure[J]. J Virol, 2017, 91(1):e01879-16.
[54]	ZHU Z, YANG F, CHEN P, et al. Emergence of novel Seneca Valley virus strains in China, 2017[J]. Transbound Emerg Dis, 2017, 64(4):1024-1029.
[55]	HAUSE B M, COLLIN E A, PEDDIREDDI L, et al. Discovery of a novel putative atypical porcine pestivirus in pigs in the USA[J]. J Gen Virol, 2015, 96(10):2994-2998.
[56]	WANG T, SUN Y, QIU H J. African swine fever:an unprecedented disaster and challenge to China[J]. Infect Dis Poverty, 2018, 7(1):111.
[57]	MARCEAU C D, PUSCHNIK A S, MAJZOUB K et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens[J]. Nature, 2016, 535(7610):159-163.
[58]	SAVIDIS G, MCDOUGALL W M, MERANER P, et al. Identification of zika virus and dengue virus dependency factors using functional genomics[J]. Cell Rep, 2016, 16(1):232-246.
[59]	PUSCHNIK A S, MAJZOUB K, OOI Y S, et al. A CRISPR toolbox to study virus-host interactions[J]. Nat Rev Microbiol, 2017, 15(6):351-364.
[60]	FU Y F, FODEN J A, KHAYTER C, et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells[J]. Nat Biotechnol, 2013, 31(9):822-826.
[61]	HSU P D, SCOTT D A, WEINSTEIN J A, et al. DNA targeting specificity of RNA-guided Cas9 nucleases[J]. Nat Biotechnol, 2013, 31(9):827-832.
[62]	ZETSCHE B, GOOTENBERG J S, ABUDAYYEH O O, et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system[J]. Cell, 2015, 163(3):759-771.
[63]	ENGREITZ J, ABUDAYYEH O, GOOTENBERG J, et al. CRISPR tools for systematic studies of RNA regulation[J]. Cold Spring Harb Perspect Biol, 2019, 11(8):a035386.
[64]	WANG Q X, LIU X, ZHOU J H, et al. The CRISPR-Cas13a gene-editing system induces collateral cleavage of RNA in glioma cells[J]. Adv Sci, 2019, 6(20):1901299.

Research Progress and Application of CRISPR/Cas9 Gene Editing Technology in Prevention and Control of Important Swine Virus Diseases

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