复方术苦芩提取液对腹泻小鼠iIELs及十二指肠INF-γ、IL-4 mRNA转录的影响

doi:10.11843/j.issn.0366-6964.2020.03.023

Abstract

Abstract: To study the effects of Zhukuqin compound extractive fluid on intestinal intraepithelial lymphocyte (iIELs) and the transcription of INF-γ and IL-4 mRNA in the duodenum of diarrhea mice. A total of 72 mice were randomly divided into six groups equally:control group, infection group, positive drug group (Astragalus polysaccharide oral liquid, the concentration was 1.2 mg·mL^-1), and drug group Ⅰ, Ⅱ, Ⅲ (Zhukuqin extractive fluid, the concentrations were 0.1, 1 and 10 mg·mL^-1, respectively). After continuously pre-administration for 7 days (0.5 mL, once daily dosing), except the control group, the mice in each group were intraperitoneally injected with E. coli (5×10⁷CFU). The time point was selected as 0 h when mice appeared diarrhea in the infection group. Six mice were killed for preparing pathological sections, the number of iIELs in the small intestine was counted, and the mRNA level of INF-γ and IL-4 in the duodenum were analyzed by quantitative real-time PCR at 6 h and 4 d, respectively. The diarrhea protective rate of positive drug group and drug group Ⅰ, Ⅱ, Ⅲ were 66.7%, 50.0%, 66.7%, and 75.0%, respectively. At 6 h, compared with control group, the number of iIELs in infection group was significantly increased, and the mRNA level of INF-γ and IL-4 were observably reduced and strengthened, respectively (P<0.01); Compared with infection group, the number of iIELs in positive drug group and drug group Ⅰ, Ⅱ, Ⅲ were decreased, and the mRNA level of INF-γ and IL-4 in positive drug group and drug group Ⅲ were augmented and lessened, respectively. At 4 d, compared with control group, the quantity of iIELs in the duodenum and ileum in infection group were markedly heightened, while that in the jejunum was dropped, and the mRNA level of INF-γ and IL-4 were dramatically enhanced (P<0.01); Compared with infection group, the number of iIELs in the duodenum and ileum in positive drug group and drug group Ⅰ, Ⅱ, Ⅲ were significantly diminished (except that in duodenum of group Ⅰ), and that in the jejunum was aggrandized, and the mRNA level of INF-γ and IL-4 in positive drug group and drug Ⅲ group were decreased. The results of the current study show that Zhukuqin compound extractive fluid can protect the diarrhea mice, via regulating the number of iIELs in the small intestine and balancing the transcription of INF-γ and IL-4 mRNA in the duodenum of mice.

Key words: Zhukuqin compound, intestinal mucosa barrier, iIELs, INF-γ, IL-4

CLC Number:

S853.74

WANG Zhongqing, WU Qiuyue, HU Qiao, ZHOU Meifang, LIU Juan. Effects of Zhukuqin Compound Extractive Fluid on iIELs and the Transcription of INF-γ and IL-4 mRNA in the Duodenum of Diarrhea Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 630-638.

References 26

[1]	吴山楠, 何翠华, 孙启明, 等. 中草药在畜禽常见疾病防治中的应用[J]. 黑龙江畜牧兽医, 2015(20):146-147.WU S N, HE C H, SUN Q M, et al. Application of Chinese herbal medicine in prevention and treatment of common diseases of livestock and poultry[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015(20):146-147. (in Chinese)
[2]	林春发, 郝永峰, 刘娟. 术苦芩总多糖对湿热泄泻仔猪小肠杯状细胞数量以及MUC-2和ITF-3 mRNA转录的影响[J]. 畜牧兽医学报, 2019, 50(6):1301-1311.LIN C F, HAO Y F, LIU J. Effect of polysaccharides from ZhuKuQin on the number of intestinal goblet cell and the transcription of MUC-2 and ITF-3 mRNA in the small intestine of damp-heat diarrhea piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(6):1301-1311. (in Chinese)
[3]	曾宇君, 赖建彬, 朱兆荣, 等. 术苦芩颗粒对湿热泻痢仔猪血清D-木糖、淀粉酶及脂肪酶的影响[J]. 中国兽医杂志, 2015, 51(1):57-59.ZENG Y J, LAI J B, ZHU Z R, et al. The effect of Zhukuqin Ganules on serum D-xylose, amylase and lipase in piglets of damp-heat and diarrhea syndrome[J]. Chinese Journal of Veterinary Medicine, 2015, 51(1):57-59. (in Chinese)
[4]	张赛奇. 葛芪复方对仔猪大肠杆菌性腹泻作用效果研究[D]. 北京:中国农业科学院, 2015.ZHANG S Q. The study of the effect of "Ge Qi" Chinese herb compound on the piglets diarrhea induced by the E. coli[D]. Beijing:Chinese Academy of Agricultural Sciences, 2015. (in Chinese)
[5]	闫金坤, 陈耀星, 王子旭. 雌激素对大鼠肠黏膜结构及上皮内淋巴细胞和杯状细胞数量、分布的影响[J]. 畜牧兽医学报, 2008, 39(9):1267-1271.YAN J K, CHEN Y X, WANG Z X. Effect of estrogen on structure of mucosal epithelium and number of intraepithelial lymphocytes and goblet cells in small intestine of rat[J]. Acta Veterinaria et Zootechnica Sinica, 2008, 39(9):1267-1271. (in Chinese)
[6]	朱兆荣, 刘娟, 杜林林, 等. 复方苦芩对小鼠免疫功能及犬IL-4 mRNA和IFN-γ mRNA表达的影响[J]. 中国兽医科学, 2012, 42(8):875-880.ZHU Z R, LIU J, DU L L, et al. Effect of Kuqin compound on immune function of experimental animals and on expressions of canine IL-4 mRNA and IFN-γ mRNA[J]. Chinese Veterinary Science, 2012, 42(8):875-880. (in Chinese)
[7]	龚妍春, 李玉萍. 黄芩在动物体内药理作用的研究进展[J]. 黑龙江畜牧兽医, 2018(1):86-89.GONG Y C, LI Y P. Advances in pharmacological effects of Scutellaria baicalensis in animals[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(1):86-89. (in Chinese)
[8]	陈希文, 赖守勋, 尹苗, 等. 22种中药多糖对猪源金黄色葡萄球菌的体外抑菌活性[J]. 江苏农业科学, 2016, 44(9):264-266, 267.CHEN X W, LAI S X, YIN M, et al. Antibacterial activity of 22 kinds of Chinese traditional medicine polysaccharides against Staphylococcus aureus from swine in vitro[J]. Jiangsu Agricultural Sciences, 2016, 44(9):264-266, 267. (in Chinese)
[9]	张瀚元, 张秀英, 施路一. 中药提取物干预鸡大肠杆菌病发病过程的研究[J]. 黑龙江畜牧兽医, 2016(3):213-215.ZHANG H Y, ZHANG X Y, SHI L Y. Study on the effect of Chinese herbal extracts on the pathogenesis of chicken Escherichia coli[J]. Heilongjiang Animal Science and Veterinary Medicine, 2016(3):213-215. (in Chinese)
[10]	段智璇, 张柏岳, 瞿慧琴, 等. 实时荧光定量PCR法分析苦参对大鼠胃肠道大肠杆菌数量的影响[J]. 贵阳中医学院学报, 2016, 38(6):1-8.DUAN Z X, ZHANG B Y, QU H Q, et al. Analysis the effect of Sophora flavescens alt on the number of Escherichia coli in the gastrointestinal tract of rats by method of real-time fluorescent quantitative PCR[J]. Journal of Guiyang College of Traditional Chinese Medicine, 2016, 38(6):1-8. (in Chinese)
[11]	武宇芳, 刘小宝, 赵二劳. 白术中黄酮的提取及其抑菌和抗氧化活性的研究[J]. 黑龙江畜牧兽医, 2017(12):148-150.WU Y F, LIU X B, ZHAO E L. Study on the extraction of flavonoids from Atractylodes atractylodes and the activities of antibacterial and antioxidant[J]. Heilongjiang Animal Science and Veterinary Medicine, 2017(12):148-150. (in Chinese)
[12]	李任军, 马艳粉, 卢一松, 等. 口服黄芪多糖增强肠道黏膜免疫及其对口蹄疫疫苗免疫的影响[J]. 中国兽医学报, 2017, 37(3):491-496.LI R J, MA Y F, LU Y S, et al. Enhancement of the gut mucosal immunity and immune response to the foot-and-mouth disease vaccine by oral administration of Astragalus polysaccharide[J]. Chinese Journal of Veterinary Science, 2017, 37(3):491-496. (in Chinese)
[13]	VITALE S, PICASCIA S, GIANFRANI C. The cross-talk between enterocytes and intraepithelial lymphocytes[J]. Mol Cell Pediatr, 2016, 3(1):20.
[14]	HAYDAY A, THEODORIDIS E, RAMSBURG E, et al. Intraepithelial lymphocytes:exploring the Third Way in immunology[J]. Nat Immunol, 2001, 2(11):997-1003.
[15]	李焰, 兰天香, 邱龙新, 等. 银杏叶复方对LPS应激断奶仔猪小肠黏膜形态及上皮内淋巴细胞和杯状细胞数量的影响[J]. 中国畜牧杂志, 2018, 54(12):109-113.LI Y, LAN T X, QIU L X, et al. Effects of Yinxingye compounds on the morphology of small intestinal mucosa and the quantity of intraepithelial lymphocytes and goblet cells in weaned piglets stressed by LPS[J]. Chinese Journal of Animal Science, 2018, 54(12):109-113. (in Chinese)
[16]	谢遵江, 刘文庆, 贺业春, 等. 小鼠肠上皮内淋巴细胞在粘膜免疫应答中的形态学研究[J]. 解剖学报, 1997, 28(3):309-313.XIE Z J, LIU W Q, HE Y C, et al. Morphologic studies on intestinal intraepithelial lymphocytes of mice in mucosal immune response[J]. Acta Anatomica Sinica, 1997, 28(3):309-313. (in Chinese)
[17]	QIU Y, WANG W S, XIAO W D, et al. Role of the intestinal cytokine microenvironment in shaping the intraepithelial lymphocyte repertoire[J]. J Leukocyte Biol, 2015, 97(5):849-857.
[18]	石玉祥, 闫金坤, 王雪敏, 等. 中药提取物对应激小鼠肠道上皮内淋巴细胞和杯状细胞数量及IL-2水平的影响[J]. 中国兽医科学, 2011, 41(1):85-88.SHI Y X, YAN J K, WANG X M, et al. Effect of the Chinese herbal extract on the number of intraepithelial lymphocytes and goblet cells and the level of IL-2 in the intestine of stressed mice[J]. Chinese Veterinary Science, 2011, 41(1):85-88. (in Chinese)
[19]	YU G J, OU W H, LIAO Z B, et al. Intestinal homeostasis of juvenile tiger puffer Takifugu rubripes was sensitive to dietary arachidonic acid in terms of mucosal barrier and microbiota[J]. Aquaculture, 2019, 502:97-106.
[20]	HE S, KAHLES F, RATTIK S, et al. Gut intraepithelial T cells calibrate metabolism and accelerate cardiovascular disease[J]. Nature, 2019, 566(7742):115-119.
[21]	REQUENA P, DADDAOUA A, GUADIX E, et al. Bovine glycomacropeptide induces cytokine production in human monocytes through the stimulation of the MAPK and the NF-κB signal transduction pathways[J]. Br J Pharmacol, 2009, 157(7):1232-1240.
[22]	刘开云, 郭刚, 解庆华, 等. 沙鼠感染幽门螺杆菌后胃粘膜INF-γ、IL-4及IL-12p40表达水平的分析[J]. 第三军医大学学报, 2005, 27(13):1320-1322.LIU K Y, GUO G, XIE Q H, et al. INF-γ, IL-4 and IL-12p40 expressions in gastric mucosa of Helicobacter pylori infected Mongolian gerbil[J]. Acta Academiae Medicinae Militaris Tertiae, 2005, 27(13):1320-1322. (in Chinese)
[23]	王旭丹, 袁学勤. T淋巴细胞与炎症性肠病[J]. 细胞与分子免疫学杂志, 2005, 21(S1):95-97, 100.WANG X D, YUAN X Q. T lymphocytes and inflammatory bowel diseases[J]. Chinese Journal of Cellular and Molecular Immunology, 2005, 21(S1):95-97, 100. (in Chinese)
[24]	ABO-AZIZA F A M, HENDAWY S H M, EL NAMAKY A H, et al. Th1/Th2 balance and humoral immune response to potential antigens as early diagnostic method of equine Strongylus nematode infection[J]. Vet World, 2017, 10(6):679-687.
[25]	BHARDWAJ R, VERMA R, DEKA D, et al. Validation of immunomodulatory effects of lipopolysaccharide through expression profiling of Th1 and Th2 biased genes in Newcastle disease virus vaccinated indigenous chicken[J]. Vet World, 2018, 11(4):437-445.
[26]	王婵, 左之才, 余树民, 等. 预防用自拟复方中药对大肠杆菌感染早期小鼠免疫功能的影响试验[J]. 中国兽医杂志, 2016, 52(3):66-68.WANG C, ZUO Z C, YU S M, et al. Effect of self-prepared traditional Chinese compounds on immune function of mice infected with Escherichia coli in early stage[J]. Chinese Journal of Veterinary Medicine, 2016, 52(3):66-68. (in Chinese)

Effects of Zhukuqin Compound Extractive Fluid on iIELs and the Transcription of INF-γ and IL-4 mRNA in the Duodenum of Diarrhea Mice

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